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p416TEF-SEC16

Plasmid

#102676

Purpose

The gene SEC16 is driven by the strong promoter TEF. Overexpression of SEC16 increases recombinant protein secretion

Depositor

Jens Nielsen

Article

Bao et al Appl Environ Microbiol. 2017 Jun 30;83(1

Insert

SEC16 (SEC16 Budding Yeast)

Expression

Yeast

Promoter

TEF

Available Since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pI12

Plasmid

#24273

Depositor

Mariano Garcia-Blanco

Article

Baraniak et al Mol Cell Biol. 2003 Dec . 23(24):9327-37

Insert

modified adenovirus exons and introns

Use

T7 for in vitro transcription

Expression

Mammalian

Available Since

March 30, 2010

Availability

Academic Institutions and Nonprofits only

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p3E-mKate2-HA no-pA

Plasmid

#80810

Purpose

hemagglutinin (HA) epitope fused to C-terminus of mKate2 without pA

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

mKateMyc p3E

Expression

Mammalian

Promoter

HA

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pEBG-GST-TRAF2

Plasmid

#44101

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 (TRAF2 Human)

Expression

Mammalian

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only
MXS_Tet

Plasmid

#62422

Purpose

MXS_chaining vector with Tet

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Tetracycline inducible promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR-AFB2

Plasmid

#83845

Purpose

Gateway donor vector for AFB2

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

AFB2 (AFB2 Mustard Weed)

Use

Gateway donor vector

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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mRFP1-36

Plasmid

#63825

Purpose

A member of an RBS library for mRFP1 with different translation rates

Depositor

Howard Salis

Article

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

mRFP1

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

J23100

Available Since

May 15, 2015

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-shNup133-1

Plasmid

#87334

Purpose

To express shRNA against human Nup133

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

shRNA against human Nup133

Use

RNAi

Expression

Mammalian

Promoter

H1

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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HCB1

Plasmid

#98582

Purpose

Huntingtin Chromobody 1: "Happ1" intrabody recognizing amino acids 41–81 of huntingtin, fused to eYFP.

Depositor

Ray Truant

Article

Maiuri et al Hum Mol Genet. 2016 Dec 25. pii: ddw395.

Insert

HCB1

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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pGGEV_4'_+nls-mCherry-Flag-_+1_BK+

Plasmid

#49313

Purpose

Golden Gate entry vector: Position 4', Flag tagged nls-mCherry with start and stop codon

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Insert

nls-mCherry-Flag

Use

Cloning vector

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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SIN40C.SFFV.sfGFP-RepEntry.iPAC

Plasmid

#100893

Purpose

Lentiviral CRISPR-Cas9 Reporter

Depositor

Dirk Heckl

Article

Labuhn et al Nucleic Acids Res. 2018 Feb 16;46(3):137

Inserts

sfGFP
PAC

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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miR-206-1/miR-133b promoter GFP

Plasmid

#20793

Depositor

David Bartel

Article

Shkumatava et al Genes Dev. 2009 Feb 15. 23(4):466-81.

Insert

mir-206-1/mir-133b (mir206-1 Zebrafish)

Use

Zebrafish expression

Tags

GFP

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only
pGP8GF-ccdB

Plasmid

#188445

Purpose

Cloning Vector and pDEST

Depositor

Britney Moss

Article

Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

Insert

ccdB

Expression

Yeast

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pH2B-mPlum/Gag

Plasmid

#102255

Purpose

co-expression of Gag and H2B Plum in mammalian cells

Depositor

Suliana Manley

Article

Gunzenhauser et al PLoS One. 2014 Dec 10;9(12):e115095. doi

Inserts

Gag
H2B Plum

Expression

Mammalian

Available Since

Nov. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pGJJ055

Plasmid

#183622

Purpose

ddPCA mutagenesis plasmid - Efficient mutant library cloning plasmid suitable for oligo synthesis, error prone PCR or nicking mutagenesis strategies.

Depositor

Ben Lehner

Article

Faure et al Nature. 2022 Apr;604(7904):175-183. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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pTol2-eab2-AG

Plasmid

#182155

Purpose

Expression of chimeric AG (N-mApple-intron-C-eGFP)

Depositor

Hui Zong

Article

Xu et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

AG

Use

Zebrafish expression

Promoter

eab2

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pTol2-eab2-GA

Plasmid

#182156

Purpose

Expression of chimeric GA (N-eGFP-intron-C-mApple)

Depositor

Hui Zong

Article

Xu et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

GA

Use

Zebrafish expression

Promoter

eab2

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-p85α_wt

Plasmid

#39496

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

p85 (PIK3R1 Bovine)

Expression

Mammalian

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
MXS_tTA2

Plasmid

#62429

Purpose

MXS_chaining vector with rTA2

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Tetracycline transactivator

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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V180 pLP Triple-Flag SD - Acceptor

Plasmid

#11707

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

Type

Empty backbone

Use

Cre/Lox; Acceptor vector

Tags

3xFLAG

Expression

Mammalian

Available Since

June 22, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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p3E-mKate2-myc no-pA

Plasmid

#80812

Purpose

myc epitope fused to C-terminus of mKate2 without pA

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

mKateHA p3E

Expression

Mammalian

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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Hox A13a

Plasmid

#36463

Depositor

Robert Ho

Article

Ahn et al Dev Biol. 2008 Oct 1;322(1):220-33. Epub

Insert

hoxA13a (hoxa13a Zebrafish)

Use

Riboprobe synthesis

Mutation

contains aa102-290; M107T

Promoter

T7

Available Since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only
Thy1.2-Roxed-Cre

Plasmid

#51276

Purpose

Expression of the Dre-dependent Roxed-Cre gene under the control of the Thy1.2 expression cassette

Depositor

Pawel Pelczar

Article

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Insert

Roxed-Cre

Promoter

Thy1.2

Available Since

March 17, 2014

Availability

Academic Institutions and Nonprofits only

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pBSV2G_P0026-sgRNA500

Plasmid

#149620

Purpose

gRNA expression in B. burgdorferi, parental vector

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

P0026

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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MXS_bGHpA

Plasmid

#62425

Purpose

MXS_chaining vector with bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Bovine growth hormone poly A

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_CAG

Plasmid

#62420

Purpose

MXS_chaining vector with CAG

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

CAG Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_minPGK

Plasmid

#62415

Purpose

MXS_chaining vector with minPGK

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Minimal PGK Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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mRFP1-1

Plasmid

#63816

Purpose

A member of an RBS library for mRFP1 with different translation rates

Depositor

Howard Salis

Article

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

mRFP1

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

J23100

Available Since

May 15, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
p3E-CFP-HA no-pA

Plasmid

#82591

Purpose

3' entry vector containing HA epitope fused to c-terminus of CFP without pA for imaging, epitope labeling/purification

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

ECFP-HA (no pA)

Promoter

None

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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V179 pLP Triple-Flag - Acceptor

Plasmid

#11706

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

Type

Empty backbone

Use

Cre/Lox; Acceptor vector

Tags

3xFLAG

Expression

Mammalian

Available Since

May 26, 2006

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pAc5.1A-Dm-bantam

Plasmid

#22788

Depositor

Elisa Izaurralde

Article

Rehwinkel et al RNA. 2005 Nov . 11(11):1640-7.

Insert

bantam (ban primary transcript, Fly)

Use

Drosophila expression

Available Since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pSUPER-retro-puro-shNup133-2

Plasmid

#87333

Purpose

To express shRNA against human Nup133

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

shRNA against human Nup133

Use

RNAi

Expression

Mammalian

Promoter

H1

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
6xHis-MBP-huCMtCas12a

Plasmid

#174680

Purpose

Expresses 6xHis-MBP tagged CMtCas12a

Depositor

Piyush Jain

Article

Nguyen et al medRxiv 2021.07.21

Insert

huCMtCas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV and NLS, 6xHis, 3xHA

Expression

Bacterial

Promoter

T7

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1A-Dm-mir-12

Plasmid

#22760

Depositor

Elisa Izaurralde

Article

Rehwinkel et al RNA. 2005 Nov . 11(11):1640-7.

Insert

micro RNA 12 (mir-12 primary transcript, Fly)

Use

Drosophila expression

Available Since

Dec. 16, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
mRFP1-9

Plasmid

#63818

Purpose

A member of an RBS library for mRFP1 with different translation rates

Depositor

Howard Salis

Article

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

mRFP1

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

J23100

Available Since

May 15, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pUCBB-lacZa

Plasmid

#32563

Depositor

Claudia Schmidt-dannert

Article

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

LacZ-alpha

Use

Synthetic Biology

Tags

His

Expression

Bacterial

Promoter

Consitutive lac

Available Since

Nov. 8, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-Char-BFP2-TSERex

Plasmid

#124792

Purpose

Expresses the proton pump rhodopsin in mammalian cells under CAG promoter

Depositor

Yulong Li

Article

Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

Insert

Char-BFP2

Expression

Mammalian

Promoter

CAG

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pSABAD544

Plasmid

#45617

Depositor

Hideo Iwai

Article

Aranko et al Nat Chem Biol. 2013 Aug 25. doi: 10.1038

Insert

GFPN-NpuDnaE_AA-GFP_C

Expression

Bacterial

Mutation

Inactivating mutations in NpuDnaE C-terminus, ste…

Promoter

pBAD

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
V181 pLPS 3' Triple-Flag - Acceptor

Plasmid

#11708

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

Type

Empty backbone

Use

Cre/Lox; Acceptor vector

Tags

3xFLAG

Expression

Mammalian

Available Since

May 26, 2006

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
V1579 pLP ProHis HTb - Acceptor

Plasmid

#11718

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

Type

Empty backbone

Use

Cre/Lox; Acceptor vector

Tags

His

Expression

Bacterial

Available Since

June 2, 2006

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
MXS_H2B

Plasmid

#62432

Purpose

MXS_chaining vector with H2B

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Histone 2B with leading Kozak sequence

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pJDC122

Plasmid

#157876

Purpose

tdTomato is expressed under the mycobacterial phsp60 heat shock promoter for in vivo and in vitro detection.

Depositor

Jeffrey Cirillo

Article

Kong et al PLoS One. 2016 Mar 2;11(3):e0149972. doi

Insert

tdTomato

Expression

Bacterial

Available Since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-sgNeo3/Cre

Plasmid

#89653

Purpose

Expresses a Neomycin-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Article

Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

Insert

gRNA targeting Neomycin

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pPS832

Plasmid

#1357

Depositor

Pamela Silver

Article

Schlenstedt et al Proc Natl Acad Sci U S A 1995 Jan 3;92(1

Insert

GSP1 (GSP1 Budding Yeast)

Expression

Yeast

Available Since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
MXS_SV40pA

Plasmid

#62426

Purpose

MXS_chaining vector with SV40pA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

SV40 poly A

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
p3E-mCherry-HA no-pA

Plasmid

#82592

Purpose

3' entry vector with HA epitope fused to C-terminus of mCherry without pA for imaging, epitope labeling/purification

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

mCherry-HA (no pA)

Promoter

None

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pAGM9121_OctaHis-GFP11

Plasmid

#153509

Purpose

OctaHis-GFP11 C-terminal Tag Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

OctaHis-GFP11

Use

Synthetic Biology

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
p3E-HA no-pA

Plasmid

#82593

Purpose

3' entry vector containing HA epitope fused to C-terminus of mCherry without pA for epitope labeling/purification

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

HA tag (no pA)

Promoter

None

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
HJP-224-actin5C^Syn21Gal80^IVS-Syn21myr-tdTomato-10UAS-mCD8GFP-p10w

Plasmid

#162481

Purpose

A bicistronic construct - FloxGal80 and UAS-mCD8GFP

Depositor

Benjamin White

Article

Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

Insert

Cre80Tom-GFP

Use

Cre/Lox

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
MXS_CMVenhancer

Plasmid

#62419

Purpose

MXS_chaining vector with CMVenhancer

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

CMV enhancer

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 9,457 results for: App