We narrowed to 22,354 results for: ESC;

1. pMH0006

   Plasmid

   #135448

   Purpose

   Human expression vector containing Ubiquitous Chromatin Opening Element (UCOE) upstream of EF1alpha promoter, dCas9 that is fused to NLS, tagBFP and a KRAB domain.

   Depositor

   Martin Kampmann, Jonathan Weissman

   Article

   Chen et al J Biol Chem. 2019 Oct 2. pii: RA119.0094

   Insert

   dCas9-BFP-KRAB
   

   Use

   Lentiviral

   Tags

   tagBFP

   Expression

   Mammalian

   Promoter

   EF1A

   Available Since

   July 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. eMscL WT

   Plasmid

   #107454

   Purpose

   eMscL WT is optimized for mammalian rodent neuronal expression to the plasma membrane through a neuron-specific promoter and a voltage-gated channel targeting motif

   Depositor

   Francesco Difato

   Article

   Soloperto et al J Cell Sci. 2018 Jan 29. pii: jcs.210393

   Insert

   bacterial mechanosensitive ion channel of large conductance (mscL Escherichia Coli)
   

   Use

   Adeno-associated viral

   Tags

   Kir2.1 ER export signal (FCYENEV) and tdTomato fl…

   Expression

   Mammalian

   Promoter

   human synapsin 1

   Available Since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDIV313

   Plasmid

   #204956

   Purpose

   AMA1 plasmid with Aspergillus optimized Mad7, hph (hygromycin) resistance marker and sgRNA targeting albA locus in A. niger

   Depositor

   Uffe Mortensen

   Article

   Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

   Inserts

   Mad7
   hph (hygromycin resistance marker)
   sgRNA (CAGCAATGCTTCCATGCAATT) targeting albA locus in A. niger flanked by a tRNA-Gly repeat
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Promoter

   Aspergillus fumigatus U3 promoter, Aspergillus ni…

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pELPS-eDHFR-YFP-T2A-Renilla

   Plasmid

   #193253

   Purpose

   Triple reporter plasmid in pELPS with eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferase

   Depositor

   Mark Sellmyer

   Article

   Sellmyer et al Mol Ther. 2020 Jan 8;28(1):42-51. doi: 1

   Insert

   The eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferase
   

   Use

   Lentiviral

   Tags

   Renilla luciferase (rLuc) and yellow fluorescent …

   Mutation

   None

   Promoter

   EF1a

   Available Since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMuLE_EXPR_CMV-eGFP_TOP-NLuc1.1_12GLI-FLuc_CBF-GLuc

   Plasmid

   #113862

   Purpose

   Triple pathway reporter, 3P-Luc; wnt-NLuc1.1, hedgehog-FLuc, notch-GLuc; plus CMV-eGFP. Gateway expression vector for lentivirus generation.

   Depositor

   Manfred Ogris

   Article

   Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

   Inserts

   CMV-eGFP
   TOP-NLuc1.1
   12GLI-Fluc
   CBF-GLuc
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSW002-Pc-TorA(sp)-mNeonGreen

   Plasmid

   #205020

   Purpose

   Broad host-range bacterial expression vector with constitutive Pc promoter. Provides E. coli TorA signal peptide in frame with mNeonGreen (codon optimized for expression in P. fluorescens); for targeting mNeonGreen to the periplasm

   Depositor

   Rosemarie Wilton

   Article

   Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

   Insert

   TorA-mNeonGreen
   

   Expression

   Bacterial

   Mutation

   mNeonGreen is codon optimized for expression in P…

   Promoter

   Pc

   Available Since

   Sept. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. RANLS-DEVD-BNES

   Plasmid

   #50840

   Purpose

   Expresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-3 biosensor, used together with plasmid 2 (BNLS).

   Depositor

   Robert Campbell

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   ddRFP A and ddRFP B
   

   Tags

   NES sequence LALKLAGLDIGS and triplicated NLS seq…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. GANES-DEVD-BNLS

   Plasmid

   #50842

   Purpose

   Expresses a tandem green ddFP heterodimer in mammalian cells, construct 1 for a green to red colour switch-based fluorescent caspase-3 biosensor, used together with a second plasmid RANLS.

   Depositor

   Robert Campbell

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   ddGFP A and ddGFP B
   

   Tags

   NES sequence LALKLAGLDIGS placed after ddGFP A an…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHtrA-TEV-His12

   Plasmid

   #137036

   Purpose

   expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi HtrA with C-terminal TEV-protease-cleavable His12

   Depositor

   Debra Hansen

   Article

   Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

   Insert

   AAC66500.2 (BB_0104 Borrelia burgdorferi B31)
   

   Tags

   His12 (TEV protease cleavable)

   Expression

   Bacterial

   Mutation

   wild type, full-length protein, contains signal p…

   Promoter

   T7

   Available Since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. NESRA-DEVD-BNLS

    Plasmid

    #50849

    Purpose

    Expresses a tandem red ddFP heterodimer in mammalian cells, construct 1 for a red to green colour switch-based fluorescent caspase-3 biosensor, used together with a second plasmid GANLS.

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    ddRFP A and ddRFP B
    

    Tags

    NES sequence LQKKLEELELDE placed after ddGFP A an…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDipA-TEV-His12

    Plasmid

    #137042

    Purpose

    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi DipA with C-terminal TEV-protease-cleavable His12

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAC66790.1 (BB_0418 Borrelia burgdorferi B31)
    

    Tags

    His12 (TEV protease cleavable)

    Expression

    Bacterial

    Mutation

    wild type, full-length protein, contains signal p…

    Promoter

    T7

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCyCEN_Lisp2mCherry_hsp70_GFP

    Plasmid

    #137169

    Purpose

    Dual fluorescent reporter for liver stage expression in Plasmodium cynomolgi

    Depositor

    Clemens Kocken

    Article

    Voorberg-van der Wel et al Commun Biol. 2020 Jan 3;3:7. doi: 10.103

    Inserts

    mCherry
    Nanoluc
    dihydrofolate reductase
    Green Fluorescent Protein
    

    Use

    Unspecified

    Tags

    T2A

    Promoter

    P. cynomolgi hsp70 and P. cynomolgi lisp2

    Available Since

    May 11, 2020

    Availability

    Academic Institutions and Nonprofits only

13. pBBA57-TEV-His12

    Plasmid

    #137033

    Purpose

    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BBA57 with C-terminal TEV-protease-cleavable His12

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAC66270.1 (BB_A57 Borrelia burgdorferi B31)
    

    Tags

    His12 (TEV protease cleavable)

    Expression

    Bacterial

    Mutation

    wild type, full-length BBA57, contains signal pep…

    Promoter

    T7

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pWPT-/mEGFP-1T-IRES-mCherry

    Plasmid

    #190190

    Purpose

    Lentiviral expression vector with altered Kozak sequence to direct EGFP expression. mCherry expression is regulated by an IRES.

    Depositor

    Alessandro Quattrone

    Article

    Ambrosini et al Nucleic Acids Res. 2022 Oct 14;50(18):10

    Inserts

    mEGFP
    mCherry
    

    Use

    Lentiviral

    Mutation

    changed EGFP Kozak sequence inserting a C>T mo…

    Promoter

    EIF1-short

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRSET-XS-VWF

    Plasmid

    #64847

    Purpose

    Expresses aa1594–1670 of VWF A2 domain flanked by Venus and Cerulean and tagged with 7X His at C terminus

    Depositor

    Sriram Neelamegham

    Article

    Dayananda et al Anal Biochem. 2011 Mar 15;410(2):206-13.

    Insert

    Venus_VWF A2 domain aa 1594-1670_Cerulean_TEV_7X His (VWF Human, Synthetic)
    

    Tags

    7X His, Cerulean, and Venus

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSW002-Pc-TorT(sp)-mNeonGreen

    Plasmid

    #205021

    Purpose

    Broad host-range bacterial expression vector with constitutive Pc promoter. Provides E. coli TorT signal peptide in frame with mNeonGreen (codon optimized for expression in P. fluorescens); for targeting mNeonGreen to the periplasm

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    TorT-mNeonGreen
    

    Expression

    Bacterial

    Mutation

    mNeonGreen is codon optimized for expression in P…

    Promoter

    Pc

    Available Since

    Sept. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. mTFP1-Farnesyl-5

    Plasmid

    #55481

    Purpose

    Localization: Plasma Membrane, Excitation: 462, Emission: 492

    Depositor

    Michael Davidson

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Insert

    Farnesyl (HRAS Human)
    

    Tags

    mTFP1

    Expression

    Mammalian

    Mutation

    encodes final 20 aa of NM_001130442.1

    Promoter

    CMV

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

18. mTFP1-MAPTau-N-10

    Plasmid

    #55498

    Purpose

    Localization: Microtubules, Excitation: 462, Emission: 492

    Depositor

    Michael Davidson

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Insert

    MAPTau (MAPT Human)
    

    Tags

    mTFP1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pCA528 CHMP4A 153-222

    Plasmid

    #108256

    Purpose

    Expresses C-terminal 153-222 residues of CHMP4A with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-6

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP4A residues 153-222 (CHMP4A Human)
    

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Contains nonnative Gly-Cys for fluorescent labeli…

    Promoter

    T7

    Available Since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCapVQ329R

    Plasmid

    #183029

    Purpose

    the patatin-like phosphodiesterase CapV variant CapVQ329R cloned in the L-arabinose inducible vector pBAD28

    Depositor

    Ute Romling

    Article

    Li et al NPJ Biofilms Microbiomes. 2022 May 11;8(

    Insert

    patatin-like phospholipase variant CapVQ329R
    

    Tags

    No

    Expression

    Bacterial

    Mutation

    glutamine 329 changed to arginine

    Promoter

    pBAD promoter

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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