Addgene

Showing: 1101 - 1120 of 1769 results

Results per page:

Sort results by:

Cre-responsive Cas9 expressing transposon library vector

Plasmid

#164138

Purpose

Backbone for transposon stgRNA library construction (library 4) (SpCas9 expression is induced upon Cre-loxP recombination.)

Depositor

Hyongbum Kim

Article

Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

human U6, EF-1a

Available since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA1 Flag ATXN1L

Plasmid

#33242

Depositor

Huda Zoghbi

Article

Bowman et al Nat Genet. 2007 Mar;39(3):373-9. Epub 20

Insert

ATXN1L (ATXN1L Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

April 17, 2014

Availability

Academic Institutions and Nonprofits only
pET21-GFP-AURKA-mCherry

Plasmid

#99876

Purpose

Expression of GFP-AURKA-mCherry for protein purification in bacteria

Depositor

Marc Tramier

Article

Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

Insert

AURKA (AURKA Human)

Use

Tags

GFP and mCherry

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 1, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDisplay-GRAB_OTmut

Plasmid

#185385

Purpose

Expresses the genetically-encoded fluorescent oxytocin(OT) control sensor GRAB_OTmut in mammalian cells

Depositor

Yulong Li

Article

Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

GPCR activation based oxytocin control sensor GRAB_OTmut

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
AID-BFP-loxP_myo2_neoR

Plasmid

#194054

Purpose

Dual-selection cassette plasmid for knocking-in AID-BFP into the C. elegans genome

Depositor

Kota Mizumoto

Article

Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

Insert

IAA17 (AXR3 Mustard Weed)

Use

Tags

bfp

Expression

Worm

Mutation

Promoter

None

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAS_4xG1 PylT sfGFP 150TAG

Plasmid

#154767

Purpose

plasmid with 4xG1 PylT cassette and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

sfGFP

Use

Tags

Expression

Mammalian

Mutation

150TAG

Promoter

EF1

Available since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-DRD2-V2tail-TevN-BLITz1-TetR-VP16

Plasmid

#89870

Purpose

expresses DRD2-iTango component in mammalian cells

Depositor

Hyungbae Kwon

Article

Lee et al Nat Methods. 2017 May;14(5):495-503. doi

Insert

DRD2-V2tail-TevN-BLITz1-TetR-VP16

Use

Tags

Expression

Mammalian

Mutation

short isoform of DRD2 (deletion of 242-270)

Promoter

CMV

Available since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEST-p70a-UTR3-deGFP-6xHis-T500

Plasmid

#92224

Purpose

Expression of deGFP using the p70a promoter and UTR3

Depositor

Lital Alfonta

Article

Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

Insert

deGFP

Use

Synthetic Biology; Cell free protein synthesis (c…

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

p70a

Available since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
XW17

Plasmid

#65835

Purpose

Punc-4-QF-3'UTR-SL2-mCherry-unc-54 (The endogenous SphI, KpnI and SacI sites in QF are removed for the convenience of cloning, most sites are compatible with pPD95.77)

Depositor

Kang Shen

Article

Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

Insert

OR74A quinic acid utilization activator (qa-1F)

Use

Tags

mCherry

Expression

Worm

Mutation

The endogenous SphI, KpnI and SacI sites in QF ar…

Promoter

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pLL7.0-iRFP670-micro

Plasmid

#135954

Purpose

Expresses an iLID micro (SspB R73Q)-iRFP670 fusion in mammalian cells.

Depositor

Antonio Amelio

Article

Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

Insert

iLID micro (SspB R73Q)

Use

Lentiviral

Tags

iRFP670

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAS_4xhyb PylT sfGFP 150TAG

Plasmid

#154771

Purpose

plasmid with 4xhybrid PylT cassette (Mx1201 G1 PylT hybrid ) and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

sfGFP

Use

Tags

Expression

Mammalian

Mutation

150TAG

Promoter

EF1

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAMV:nAChRgamma-eGFP

Plasmid

#29693

Depositor

Henry Lester

Article

Pantoja et al Biophys J. 2009 Jan . 96(1):226-37.

Insert

GFP tagged Mus musculus cholinergic receptor, nicotinic, gamma (Chrng Mouse)

Use

Tags

AMV promotor and poly(A) tail

Expression

Bacterial

Mutation

eGFP inserted after position S380

Promoter

Available since

July 16, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pRP_8xgRNA-FLuc

Plasmid

#135937

Purpose

Firefly luciferase reporter containing 8 copies of a gRNA binding site for light-inducible dCas9 activation.

Depositor

Antonio Amelio

Article

Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

Insert

8 copies of gRNA binding site (5'-AAAGGTCGAGAAACTGCAAA-3')

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

minCMV

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pKT2/CAGXSP/GFPCD63

Plasmid

#231709

Purpose

Stably express CD63 fused with GFP in mammalian cells via the sleeping beauty transposon system.

Depositor

Masamitsu Kanada

Article

Li et al Sci Rep. 2024 Nov 9;14(1):27432. doi: 10

Insert

CD63 (CD63 Human)

Use

Tags

gfp

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28:LanII-57

Plasmid

#225074

Purpose

Expresses two precursor peptides and the lanthipeptide synthetase from LanII-57 (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA(A) (LanII-57)

Use

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRXIP-GFP-PKD C1ab

Plasmid

#221755

Purpose

Stable expression of GFP-PKD C1ab in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

Prkd1(138-343) (Prkd1 Mouse)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

amino acids 138-343

Promoter

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pEVOL-tac-EcTyrRS-VSMA*-lpp-tRNA_CUA^EcTyr

Plasmid

#218766

Purpose

encodes EcTyrRS-VSMA* and EcTyr-tRNA-TAG

Depositor

Abhishek Chatterjee

Article

Ficaretta et al ACS Chem Biol. 2024 Jun 24. doi: 10.1021

Insert

EcTyrRS-VSMA*

Use

Tags

Expression

Bacterial

Mutation

Y37V, D167G, D182S, F183M, L186A, D265R

Promoter

tacI

Available since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pEVOL-tac-EcTyrRS-pAAFRS-9-lpp-tRNA_CUA^EcTyr

Plasmid

#218767

Purpose

encodes EcTyrRS-pAAFRS-9 and EcTyr-tRNA-TAG

Depositor

Abhishek Chatterjee

Article

Ficaretta et al ACS Chem Biol. 2024 Jun 24. doi: 10.1021

Insert

EcTyrRS-pAAFRS-9

Use

Tags

Expression

Bacterial

Mutation

Y37G, L71V, D182C, F183Y, L186C, D265R

Promoter

tacI

Available since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-Nova1-GFP

Plasmid

#217033

Purpose

Overexpression of Nova in DRG neurons promotes the "mature" splicing pattern observed in CNS neurons

Depositor

Chaolin Zhang

Article

Weyn-Vanhentenryck et al Nat Commun. 2018 Jun 6;9(1):2189. doi: 1

Insert

Nova1 (Nova1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
TDP-43 mRuby K84ONBK

Plasmid

#216226

Purpose

TDP-43 with a C-terminal mRuby tag and Lysine 84 in the NLS mutated to a TAG stop codon for amber codon suppression.

Depositor

Jennifer Lee

Article

Shadish et al Biophys Chem. 2024 Jan 23;307:107191. do

Insert

Transactive response DNA binding protein of 43 kDa (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

Lysine 84 mutated to a TAG stop codon

Promoter

CMV Promoter

Available since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge