We narrowed to 28,416 results for: REP

1. pCAG-mcherry-WIPI2dK128E-cs-TEV-STREP

   Plasmid

   #171844

   Purpose

   Mammalian expression of human WIPI2d K128E with N-terminal mCherry and C-terminal Strep

   Depositor

   James Hurley

   Article

   Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

   Insert

   WIPI2d (WIPI2 Human)
   

   Use

   Tags

   Strep and mCherry

   Expression

   Mammalian

   Mutation

   K128E

   Promoter

   Available since

   Oct. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-2xFLAG-2xSTREP-CCND1(P287S)

   Plasmid

   #172644

   Purpose

   Expresses 2xFLAG-2xSTREP-tagged cyclin D1(P287S) in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND1 (CCND1 Human)
   

   Use

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Pro287Ser

   Promoter

   CMV

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-2xFLAG-2xSTREP-CCND1(T286I)

   Plasmid

   #172645

   Purpose

   Expresses 2xFLAG-2xSTREP-tagged cyclin D1(T286I) in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND1 (CCND1 Human)
   

   Use

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Thr286Ile

   Promoter

   CMV

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-2xFLAG-2xSTREP-CCND1(P287L)

   Plasmid

   #172646

   Purpose

   Expresses 2xFLAG-2xSTREP-tagged cyclin D1(P287L) in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND1 (CCND1 Human)
   

   Use

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Pro287Leu

   Promoter

   CMV

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBABE-2xFLAG-2xSTREP-CCND1-Puro

   Plasmid

   #172634

   Purpose

   Retroviral vector for the constitutive, near-physiological expression of 2xFLAG-2xSTREP-tagged cyclin D1 and a puromycin resistance marker in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND1 (CCND1 Human)
   

   Use

   Retroviral

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET15b p97-Strep-His E314Amb

   Plasmid

   #169021

   Purpose

   Expresses p97-Strep-His E314Amb (incorporates noncanonical amino acid at position 314 via amber suppression) in bacteria

   Depositor

   Hemmo Meyer

   Article

   Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

   Insert

   p97
   

   Use

   Tags

   His and SBP

   Expression

   Bacterial

   Mutation

   E314TAG

   Promoter

   T7

   Available since

   July 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. C234-E24: attB2r-Strep2-attB3

   Plasmid

   #162913

   Purpose

   Gateway attB2r/attB3 entry clone for construction of C-terminal Strep2 tagged fusion proteins

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   Strep2 epitope tag
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLJM1-zeo-2xStrep-ORF68 C52A

   Plasmid

   #162660

   Purpose

   Lentiviral vector for stable expression of N-terminal 2xStrep-tagged ORF68 C52A in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

   Insert

   ORF68
   

   Use

   Lentiviral

   Tags

   N-terminal 2xStrep-Tag II

   Expression

   Mammalian

   Mutation

   Amino acid C52 mutated to alanine

   Promoter

   CMV

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA4/TO-2xStrep-ORF68 K450A/R451A

   Plasmid

   #162638

   Purpose

   Expresses N-terminal 2x-Strep-tagged ORF68 K450A/R451A in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

   Insert

   ORF68
   

   Use

   Tags

   N-terminal 2xStrep-Tag II

   Expression

   Mammalian

   Mutation

   Amino acids K450 and R451 mutated to alanine

   Promoter

   CMV

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA4/TO-2xStrep-ORF68 R14A/K310A

    Plasmid

    #162642

    Purpose

    Expresses N-terminal 2x-Strep-tagged ORF68 R14A/K310A in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Use

    Tags

    N-terminal 2xStrep-Tag II

    Expression

    Mammalian

    Mutation

    Amino acids R14 and K310 mutated to alanine

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA4/TO-2xStrep-ORF68 K395A/K396A

    Plasmid

    #162643

    Purpose

    Expresses N-terminal 2x-Strep-tagged ORF68 K395A/K396A in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Use

    Tags

    N-terminal 2xStrep-Tag II

    Expression

    Mammalian

    Mutation

    Amino acids K395 and K396 mutated to alanine

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA4/TO-2xStrep-UL32 C308A/C309A

    Plasmid

    #162646

    Purpose

    Expresses N-terminal 2x-Strep-tagged UL32 C308A/C309A in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    UL32
    

    Use

    Tags

    N-terminal 2xStrep-Tag II

    Expression

    Mammalian

    Mutation

    Amino acids C308 and C309 mutated to alanine

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA4/TO-2xStrep-ORF68 C191A/C192A

    Plasmid

    #162632

    Purpose

    Expresses N-terminal 2x-Strep-tagged ORF68 C191A/C192A in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Use

    Tags

    N-terminal 2xStrep-Tag II

    Expression

    Mammalian

    Mutation

    Amino acids C191 and C192 mutated to alanine

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPD027 Reporter Template v1 (Near Spaced)

    Plasmid

    #138751

    Purpose

    Template for COMET promoters, expresses EYFP reporter upon promoter activation.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPD032 Reporter Template v1 (Mid Spaced)

    Plasmid

    #138753

    Purpose

    Template for COMET promoters, expresses EYFP reporter upon promoter activation.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPD033 Reporter Template v1 (Far Spaced)

    Plasmid

    #138754

    Purpose

    Template for COMET promoters, expresses EYFP reporter upon promoter activation.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPICZ-His6-K2P4.1A-mCherry-StreptagII

    Plasmid

    #158743

    Purpose

    Expresses K2P4.1 isoform A with TEV protease cleavable N- and C-terminal affinity tags.

    Depositor

    Arthur Laganowsky

    Article

    Schrecke et al Nat Chem Biol. 2021 Jan;17(1):89-95. doi

    Insert

    K2P4.1-A (KCNK4 Human)
    

    Use

    Tags

    His6 and mCherry with Streptag II

    Expression

    Yeast

    Mutation

    Residues 1-264 with N-linked glycosylation sites …

    Promoter

    AOX1

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPICZ-His6-K2P4.1B-mCherry-StreptagII

    Plasmid

    #158744

    Purpose

    Expresses K2P4.1 isoform B with TEV protease cleavable N- and C-terminal affinity tags.

    Depositor

    Arthur Laganowsky

    Article

    Schrecke et al Nat Chem Biol. 2021 Jan;17(1):89-95. doi

    Insert

    K2P4.1-B (KCNK4 Human)
    

    Use

    Tags

    His6 and mCherry with Streptag II

    Expression

    Yeast

    Mutation

    Residues 1-290 and N-linked glycosylation sites r…

    Promoter

    AOX1

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFD-MBP-CS(TEV)-StrepTag-II-AI(TVMV)

    Plasmid

    #137110

    Purpose

    iFLinkC Linker cloning

    Depositor

    Viktor Stein

    Article

    Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

    Insert

    MBP-CS(TEV)-StrepTag-II-AI(TVMV)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA4/TO-ORF66 200-429-2xStrep

    Plasmid

    #130955

    Purpose

    Expresses KSHV ORF66 a.a. 200-429 with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

    Insert

    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    

    Use

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    ORF66 a.a. 200-429

    Promoter

    CMV

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge