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pDGE661

Plasmid

#153259

Purpose

M5 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pMpGWB409

Plasmid

#68674

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

3xFLAG

Expression

Plant

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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EC0_2_VP64

Plasmid

#163714

Purpose

Plasmid encoding VP64 - SV40 NLS as a Type 4a part to be used in the Dueber YTK system (MoClo kit)

Depositor

Yvonne Nygård

Article

Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

Insert

VP64 + SV40 NLS

Use

Synthetic Biology; Moclo vector level 0 (type 4a)

Available Since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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Osa-pCAMBIA-1301-STTM394

Plasmid

#84204

Purpose

Disturb the expression of miR394 in rice

Depositor

Guiliang Tang

Article

Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

Insert

miR394

Expression

Bacterial, Plant, and Yeast…

Promoter

2x35S

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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MTK3_027

Plasmid

#123742

Purpose

Encodes bicistronic ABI gene expression system as a Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

VP16::PYLcs-T2A-GAL4DBD::ABIcs

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pRAN334

Plasmid

#120814

Purpose

E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of cyan fluorescent protein (CfpOpt)

Depositor

Craig Ellermeier, David S. Weiss

Article

Ransom et al J Bacteriol. 2014 Jun;196(12):2290-300.

Insert

cyan fluorescent protein, codon-optimized for low GC organisms

Expression

Bacterial

Promoter

Ptet

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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MTK5_008

Plasmid

#123852

Purpose

Encodes ConRE' and the chicken hypersensitivity site 4 insulator as a Type 5 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector RE'-2xHS4

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pFGC5941-PacI-ath-STTM398

Plasmid

#84124

Purpose

target miRNA398 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM398

Expression

Plant

Promoter

2x35S

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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MTK1_027

Plasmid

#123689

Purpose

Encodes ConL5 and human E4 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L5-E4 insulator

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK1_028

Plasmid

#123690

Purpose

Encodes ConL6 and human F1 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L6-F1 insulator

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK1_029

Plasmid

#123691

Purpose

Encodes ConL7 and human B2 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L7-B2 insulator

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046841

Plasmid

#145578

Purpose

Bacterial Expression plasmid for SARS-CoV-2 helicase

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 helicase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;FLAG_2

Expression

Bacterial

Mutation

Escherichia coli recode 1

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available Since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pFGC5941-PacI-ath-STTM395

Plasmid

#84121

Purpose

target miRNA395 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM395

Expression

Plant

Promoter

2x35S

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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AEB-DNT3

Plasmid

#63852

Purpose

This pFTV1 vector containsa 2,4,dinitrotoluene (DNT) binding riboswitch, controlling the translation initaition rate of mRFP1 gene.

Depositor

Howard Salis

Article

Espah Borujeni et al Nucleic Acids Res. 2015 Nov 30. pii: gkv

Insert

DNT3 DNT riboswitch

Use

Synthetic Biology

Promoter

AEB3

Available Since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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Osa-pCAMBIA-1301-STTM166

Plasmid

#84197

Purpose

Disturb the expression of miR166 in rice

Depositor

Guiliang Tang

Article

Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

Insert

miR166

Expression

Bacterial, Plant, and Yeast…

Promoter

2x35S

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pFGC5941-PacI-ath-STTM773a/b

Plasmid

#84141

Purpose

target miRNA773a/b for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM773a/b

Expression

Plant

Promoter

2x35S

Available Since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pBL119-2

Plasmid

#104336

Purpose

Expresses thsR from a TetR regulated promoter under ATC control. Expresses sfGFP from a thsR regulated promoter. Expresses mCherry from a constituative promoter.

Depositor

Jeffrey Tabor

Article

Landry et al Nat Commun. 2018 Apr 12;9(1):1433. doi:

Insert

Thiosulfate response regulator

Expression

Bacterial

Promoter

PLTetO1

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pIE2-AD

Plasmid

#21603

Depositor

Takahiro Kusakabe

Article

Mon et al Anal Biochem. 2009 Sep 15;392(2):180-2.

Insert

NF-kappa-B p65 subunit (Rela Mouse)

Use

Gateway destination vector

Tags

Flag, Gateway destination cassette, and NLS

Expression

Insect

Available Since

Sept. 22, 2009

Availability

Academic Institutions and Nonprofits only

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pFUSE-B_P2rP4

Plasmid

#97185

Purpose

For fusion gene cloning. Recombine Gene B into this Gateway Entry Vector

Depositor

Kenneth Scott

Article

Lu et al Cancer Res. 2017 May 16. pii: canres.274

Type

Empty backbone

Use

Gateway

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pFGC5941-PacI-ath-STTM408

Plasmid

#84132

Purpose

target miRNA408 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM408

Expression

Plant

Promoter

2x35S

Available Since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

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