We narrowed to 24,186 results for: CRISPR
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Plasmid#239515PurposeExpress the lower portion of melvaonate pathway enzymes (Erg12p, Erg8p, and Erg19p) in the peroxisome; chromosomal integration by CRISPRDepositorInsertScErg12p-ePTS1
ExpressionYeastAvailable SinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
1203A-CMV-5’DR-EGFP-bGHpolyA
Plasmid#221452PurposeEGFP reporter construct with single unprocessed direct repeat (35nt) at 5' endDepositorInserteGFP
ExpressionMammalianAvailable SinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
1203B-CMV-3’DR-EGFP-bGHpolyA
Plasmid#221453PurposeEGFP reporter construct with single unprocessed direct repeat (35nt) at 3' endDepositorInserteGFP
ExpressionMammalianAvailable SinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRPCE
Plasmid#193712PurposeExpresses cytosine deaminase linked deactivated-Cas9 from RK2 origin with RFP-marked sgRNA insertion site and kanR/sacB selection/counterselectionDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceFeb. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAS-mak10 gRNA-MAK10Δ
Plasmid#160384PurposeFor CRISPR-mediated deletin og MAK10 in yeastDepositorInsertMAK10
ExpressionYeastPromoterPGK1Available SinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pFG001
Plasmid#62816PurposeBacterial constitutive gRNA expression plasmidDepositorInsertgRNA
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterPN25Available SinceNov. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDAC565
Plasmid#195242PurposeMammalian expression of Csm-GFP complex (RNase mut) from separate promoters (Used for RNA imaging)DepositorInsertFLAG-NLS-Csm1; FLAG-NLS-Csm2, FLAG-NLS-Csm3(RNase mut)-GFP; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA
ExpressionMammalianAvailable SinceJan. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
FokI-dCas9
Plasmid#52970PurposeExpresses Fok1 nuclease domain fused to catalytically inactive Cas9 DNA-binding domain in mammalian cellsDepositorInsertFok1 fused to dCas9
UseCRISPRTagsFok1 nuclease (homodimeric wildtype) and NLSExpressionMammalianMutationD10A and H840A in Cas9PromoterCMVAvailable SinceMay 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-puro-v2
Plasmid#127458PurposeEntry plasmid for guide cloningDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterEF1aAvailable SinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
p-dCas9-LSD1-Hygro
Plasmid#104406Purposetransient expression of dCas9-LSD1 fusion proteinDepositorInsertLSD1 (KDM1A Human)
UseCRISPRExpressionMammalianMutationIRATp970A0364D (Source Bioscience) has a P405H ch…PromoterCMV promoterAvailable SinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
DS-SPcas
Plasmid#48645PurposeBacterial S. pyogenes Cas9 (SP) + tracrRNA expression, cloDF13/spectinomycinDepositorInsertsCas9
tracrRNA precursor
UseCRISPRPromoterproC and tracrRNA promoterAvailable SinceDec. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMJ920
Plasmid#42234DepositorInsertCas9
UseCRISPRTagsGFP, HA epitope, and NLS (nuclear localization si…ExpressionMammalianMutationcodon-optimized synthetic DNA sequencePromoterCMVAvailable SinceMarch 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHK002.2
Plasmid#235461PurposeMessage phagemid carrying sgRNA2 (prom. J23119, backbone pBR322)DepositorInsertsgRNA2
UseSynthetic BiologyAvailable SinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti_dCas9-KRAB-MeCP2_Blast
Plasmid#221501PurposeLentiviral construct for expression of the dCas9-KRAB-MeCP2 CRISPRi vectorDepositorInsertdCas9-KRAB-MeCP2-T2A-Blast
UseCRISPR and LentiviralExpressionMammalianMutationCas9 mutations to make dCas9=D10A+D839A+H840A+N86…PromoterCMVAvailable SinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB_TRE3G_dCas9-5xGCN4_Hygro
Plasmid#235572PurposeDox-inducible expression of dCas9-GCN4 & genomic integrationDepositorInsertdCas9-5xGCN4
UseCRISPRMutationD10A; H840AAvailable SinceApril 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTFE3-donor
Plasmid#112377PurposeCRISPR donor plasmid to tag human transcription factor TFE3 with GFPDepositorInsertTFE3 homology arms flanking EGFP-IRES-Neo cassette (TFE3 Human)
UseCRISPRMutationhg38:X:49030928:T>A,hg38:X:49030929:A>T,hg3…Available SinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKM197
Plasmid#132665PurposepyrE-targeted CRISPR-Cas9 control plasmid. Cas9 expression is controlled by the xylR promoter and results in improved conjugation efficiency. Induction with xylose results in a pyrE mutant.DepositorInsertUpstream & Downstream pyrE deletion region
UseE. coli - c. difficile shuttle vectorAvailable SinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIKU801-CrPEPC1
Plasmid#92342PurposeExpresses sgRNA for targeting CrPEPC1 gene, paromomycin resistanceDepositorInsertsgRNA-CrPEPC1
UseCRISPRExpressionPlantPromoterAtU6-26Available SinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGRB
Plasmid#71539Purposefor expression of gRNA in E. coliDepositorTypeEmpty backboneExpressionBacterialPromoterJ231196Available SinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only