We narrowed to 12,204 results for: 112

1. pAF138

   Plasmid

   #135923

   Purpose

   All-in-one Sleeping Beauty vector for Expression for Tasmanian devil TNFRSF9-mCitrine fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFRSF9).

   Depositor

   Andrew S. Flies

   Article

   Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab

   Insert

   TNFRSF9 fused to mCitrine
   

   Use

   Transposon

   Tags

   mCitrine

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-GG-hUbC-EBPF2-gRNA-SOX4

   Plasmid

   #185552

   Purpose

   Lentiviral backbone with EBFP2 reporter and gRNA targeting SOX4

   Depositor

   Andrew Wilson

   Article

   Werder et al Sci Adv. 2022 Jul 15;8(28):eabo6566. doi

   Insert

   SOX4 gRNAs
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFPC2-gephyrin S268A/270A

   Plasmid

   #199608

   Purpose

   Encodes N-terminal eGFP-tagged P1-gephyrin S268/270A for expression in mammalian cells.

   Depositor

   Shiva Tyagarajan

   Article

   Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

   Insert

   Gephyrin (Gphn Rat)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S268A/S270A

   Promoter

   CMV

   Available Since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. TP53-KO_gRNA_2

   Plasmid

   #195131

   Purpose

   TP53-targeting gRNA in the LRG2.1 backbone

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   TP53 KO gRNA 2 (TP53 Human)
   

   Expression

   Mammalian

   Available Since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Gal4-VP16-HSF1 WT

   Plasmid

   #71726

   Purpose

   plasmid expressing fusion construct of Gal4 BDB VP16 AD and HSF1 WT RD

   Depositor

   Lea Sistonen

   Article

   Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

   Insert

   Gal4 DBD HSF1 VP16 AD (HSF1 Budding Yeast, Human, Herpes Simplex Virus protein VP16)
   

   Expression

   Mammalian

   Available Since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSLIKneo 3XFLAG-wtGATA6-3XAU1_RNAi resistant

   Plasmid

   #72616

   Purpose

   Expresses an inducible and RNAi resistant, N-terminally 3X-FLAG tagged, C-terminally 3X-AU1 tagged wtGATA6

   Depositor

   Kevin Janes

   Article

   Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

   Insert

   GATA Binding Protein 6 (GATA6 Human)
   

   Use

   Lentiviral

   Tags

   3XAU1 and 3XFLAG

   Expression

   Mammalian

   Promoter

   TRE tight

   Available Since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-DEUT RUVBL1 Delete

   Plasmid

   #159144

   Purpose

   Expression and protien purification of mutated RUVBL1

   Depositor

   Eric Erquan Zhang

   Article

   Ju et al Sci Transl Med. 2020 May 6;12(542). pii:

   Insert

   RUVBL1 (RUVBL1 Human)
   

   Expression

   Bacterial

   Mutation

   amino acid 1-463, Glu134-Glu237 mutated to GPPG

   Available Since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCR3-FLAG-Gephyrin E

   Plasmid

   #68819

   Purpose

   Flag tagged mammalian expression of E domain

   Depositor

   Shiva Tyagarajan

   Article

   Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

   Insert

   Gephyrin (Gphn Rat)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   internal EcoRI site has silent mutation

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3-HA-BHLHE40(S243A/E248A)

   Plasmid

   #110155

   Purpose

   Expression of human BHLHE40 (S243A/E248A) in mammalian cells

   Depositor

   Daniele Guardavaccaro

   Article

   Kim et al Mol Cell Biol. 2014 Nov 15;34(22):4177-8

   Insert

   BHLHE40 (basic helix-loop-helix family member e40) (BHLHE40 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   S243A/E248A

   Promoter

   CMV

   Available Since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBS L30 Ruby3-Rab7A

    Plasmid

    #135651

    Purpose

    Rab7A fused to the red fluorescent protein Ruby3 in N-ter under control of a weak L30 promoter.

    Depositor

    Raphael Gaudin

    Article

    Deffieu et al Sci Adv. 2021 Jan 8;7(2). pii: 7/2/eaba7

    Insert

    Rab7A (RAB7A Human)
    

    Tags

    Ruby3

    Expression

    Mammalian

    Promoter

    L30

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. 3159_pETcon-SARS-CoV-2-RBD-L452R-T478K

    Plasmid

    #184407

    Purpose

    yeast surface display of the SARS-CoV-2 Delta variant RBD

    Depositor

    Jesse Bloom

    Article

    Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

    Insert

    SARS-CoV-2 Delta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
    

    Tags

    HA and c-Myc

    Expression

    Yeast

    Mutation

    L452R+T478K

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA3 Flag MFF iso5 S172A

    Plasmid

    #74382

    Purpose

    Flag tagged human S172A MFF isoform 5 mutant

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Ser 172 Ala (numbering based on MFF isoform 1)

    Available Since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pTT5-hSDC1-CS2

    Plasmid

    #52367

    Purpose

    expresses human Syndecan-1 point mutant at S194A in HEK293-EBNA1 (293E) suspension culture.

    Depositor

    Gordon Laurie

    Article

    Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1

    Insert

    SDC1 (SDC1 Human)
    

    Expression

    Mammalian

    Mutation

    R95Q and S194A (according to numbering in Fig. 2A…

    Available Since

    Dec. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pCDNA3 Flag MFF iso5 S155,172A

    Plasmid

    #74383

    Purpose

    Flag tagged human S155,172A MFF isoform 5 mutant

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    See 155 Ala, Ser 172 Ala (numbering based on MFF …

    Available Since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pEN_TT 3XFLAG-wtGATA6-3XAU1_RNAi resistant

    Plasmid

    #72922

    Purpose

    Gateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged, RNAi resistant human wtGATA6

    Depositor

    Kevin Janes

    Article

    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

    Insert

    GATA Binding Protein 6 (GATA6 Human)
    

    Use

    Entry vector for gateway cloning

    Tags

    3XAU1 and 3XFLAG

    Expression

    Bacterial

    Promoter

    TRE tight

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. Flag‐mTim3 (T2 truncation)

    Plasmid

    #49211

    Purpose

    expresses Flag tagged mouse Tim3 (T2 truncation)

    Depositor

    Lawrence Kane

    Article

    Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

    Insert

    Tim3 T2 truncation (Havcr2 Mouse)
    

    Tags

    FLAG and signal sequence

    Expression

    Mammalian

    Mutation

    T2 truncation ( lacks all but one tyrosine, which…

    Promoter

    EF1a

    Available Since

    Dec. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET41s-GST-ATF6a-AD

    Plasmid

    #64774

    Purpose

    bacterial expression of GST-tagged ATF6 (1-150)

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

    Insert

    ATF6a-AD (1-150) (ATF6 Human)
    

    Tags

    GST

    Expression

    Mammalian

    Mutation

    C-term deletion

    Available Since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pAF161

    Plasmid

    #135926

    Purpose

    All-in-one Sleeping Beauty vector for Expression for Tasmanian devil TNFRSF9-mCerulean3 fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFRSF9).

    Depositor

    Andrew S. Flies

    Article

    Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab

    Insert

    TNFRSF9 fused to mCerulean3
    

    Use

    Transposon

    Tags

    mCerulean3

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5-Flag-Med25-VWA

    Plasmid

    #64772

    Purpose

    Mammalian expression of Flag-tagged Med25-VWA

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

    Insert

    Med25-VWA (MED25 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    C-terminal deletion

    Available Since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pEN_TTmiRC2_p53DD

    Plasmid

    #136520

    Purpose

    Used as a donor vector containing N-term 3xFLAG to clone into pSLIK

    Depositor

    Kevin Janes

    Article

    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

    Insert

    p53DD (Trp53 Mouse)
    

    Use

    Entry vector for gateway cloning

    Tags

    3x FLAG

    Mutation

    This construct was created by PCR from the pBabe …

    Available Since

    Oct. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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