Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 30,236 results for: ple

Showing: 11341 - 11360 of 30236 results

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pXD80ENSF14-Pct3-ElVadh(cHis6)-Accs(ElDSM2243)

Plasmid

#233871

Purpose

Expresses His-tagged Eggerthella lenta DSM 2243 El Vadh with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

El Vadh (Elen_0497 Eggerthella lenta)

Tags

His

Expression

Bacterial

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF12-Pct3-(–)-Eldh1(cHis6)-Accs(ElA2)

Plasmid

#233870

Purpose

Expresses His-tagged Eggerthella lenta A2 (–)-Eldh1 with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(–)-Eldh1 (Elen_0511 Eggerthella lenta)

Tags

His

Expression

Bacterial

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF10-Pct3-(+)-Eddh(cHis6)-Accs(ElA2)

Plasmid

#233869

Purpose

Expresses His-tagged Eggerthella lenta A2 (+)-Eddh with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(+)-Eddh (Elen_0501 Eggerthella lenta)

Tags

His

Expression

Bacterial

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pXD70EN19-Pct5-GPA_25810/25800-(+)-Eldh1/2(GpDSM19378)

Plasmid

#225305

Purpose

Expresses Gordonibacter pamelaeae DSM 19378 (+)-Eldh1 and (+)-Eldh2 in Eggerthella lenta DSM 2243 with a cumate inducible promoter

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(+)-Eldh1, (+)-Eldh2

Expression

Bacterial

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 WTSLFD

Plasmid

#232980

Purpose

Galactose iduced expression of Gcn4 WTSLFD+ in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 WTSLFD+

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

F108W, E109T, Y110S, E111L, N112F, L113D

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 Aro3+HA

Plasmid

#232990

Purpose

Galactose iduced expression of Gcn4 Aro3+HA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 Aro3+

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

D103Y, N112T, L113Y, S117F, K140R

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 Aro2+HA

Plasmid

#232989

Purpose

Galactose iduced expression of Gcn4 Aro2+HA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 Aro2+

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

N116F, S117E, E119N, P129Q, T132Y

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 Aro2+GFP

Plasmid

#233001

Purpose

Galactose iduced expression of Gcn4 Aro2+GFP in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 Aro2+

Tags

HA-GFP-HA and TEV cleavage site

Expression

Yeast

Mutation

N116F, S117E, E119N, P129Q, T132Y

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 solvvol

Plasmid

#232976

Purpose

Galactose iduced expression of Gcn4 solvvol in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 solvvol

Tags

TEV cleavage site

Expression

Yeast

Mutation

S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 S/ArotoL

Plasmid

#232979

Purpose

Galactose iduced expression of Gcn4 S/ArotoL in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 S/ArotoL

Tags

TEV cleavage site

Expression

Yeast

Mutation

S101L, S104L, F108L, Y110L, S117L, S136L, S144L

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 ILVtoED WHA

Plasmid

#232995

Purpose

Galactose iduced expression of Gcn4 ILVtoED W+HAin yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 ILVtoED W+

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

N126W, I128E, V130E, V135E, L137D, I142D

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 9acidAHA

Plasmid

#232997

Purpose

Galactose iduced expression of Gcn4 9acidAHA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 9acidA

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

E109A, E111A, E114A, D115A, E119A, D125A, D127A, …

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 9acidA

Plasmid

#232967

Purpose

Galactose iduced expression of Gcn4 9acidA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 9acidA

Tags

TEV cleavage site

Expression

Yeast

Mutation

E109A, E111A, E114A, D115A, E119A, D125A, D127A, …

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 9acidAGFP

Plasmid

#233009

Purpose

Galactose iduced expression of Gcn4 9acidAGFP in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 9acidA

Tags

HA-GFP-HA and TEV cleavage site

Expression

Yeast

Mutation

E109A, E111A, E114A, D115A, E119A, D125A, D127A, …

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG415Gal_Gcn4 Aro3+GFP

Plasmid

#233002

Purpose

Galactose iduced expression of Gcn4 Aro3+GFP in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 Aro3+

Tags

HA-GFP-HA and TEV cleavage site

Expression

Yeast

Mutation

D103Y, N112T, L113Y, S117F, K140R

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG415Gal_Gcn4 ILVtoED

Plasmid

#232957

Purpose

Galactose iduced expression of Gcn4 ILVtoED in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 ILVtoED

Tags

TEV cleavage site

Expression

Yeast

Mutation

L123D, I128E, V130E, V135E, L137D, I142D

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG415Gal_Gcn4 ILVtoED WGFP

Plasmid

#233007

Purpose

Galactose iduced expression of Gcn4 LVtoED W+GFPin yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 ILVtoED W+

Tags

HA-GFP-HA and TEV cleavage site

Expression

Yeast

Mutation

N126W, I128E, V130E, V135E, L137D, I142D

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG415Gal_Gcn4 ILVtoAS

Plasmid

#232959

Purpose

Galactose iduced expression of Gcn4 ILVtoAS in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 ILVtoAS

Tags

TEV cleavage site

Expression

Yeast

Mutation

L123S, I128S, V130A, V135A, L137S, I142S

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 solvvol W

Plasmid

#231878

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 solvvol W

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 solvvol W

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

Promoter

T7

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 solvvol

Plasmid

#231877

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 solvvol

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 solvvol

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

Promoter

T7

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 11341 - 11360 of 30236 results

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