Addgene

We narrowed to 4,777 results for: Mos

Showing: 2241 - 2280 of 4777 results

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Dll4ICD-HPC4 pBacPAK8.3

Plasmid

#17335

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2007 Dec 12. ():.

Insert

Delta-like 4 (Dll4 Mouse)

Use

Tags

HPC4

Expression

Insect

Mutation

Promoter

Available since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
pET28b_Q69L

Plasmid

#186272

Purpose

Expression vector of His-RanQ69L in E.coli

Depositor

Masahito Ikawa

Article

Mori et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

Insert

GTP-binding nuclear protein Ran (Ran Mouse)

Use

Tags

His-tagged at N terminal

Expression

Bacterial

Mutation

Changed Q69 into L

Promoter

T7 promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pAB076i3

Plasmid

#79198

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI-SH2ABL1

Use

Tags

Expression

Bacterial

Mutation

Promoter

PlacZ-opt (OR1); Ppro1

Available since

April 11, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE161

Plasmid

#118509

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mTurquoise2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pSL1180-HR-PUbEBFP2-NLS

Plasmid

#176682

Purpose

Expression of nuclear localized EBFP2 under Ae. aegypti Polyubiquitin promoter (AAEL003888), EBFP2 version of pSL1180-HR-PUbECFP (Addgene #47917)

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Insert

EBFP2

Use

Tags

Expression

Insect

Mutation

Promoter

Ae. aegypti poly-ubiquitin (AAEL003888)

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX303 GFP-PIK3C2B

Plasmid

#162001

Purpose

Lentiviral expression vector containing PIK3C2B tagged with GFP

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

PIK3C2B (PIK3C2B Human)

Use

Lentiviral

Tags

eGFP

Expression

Mutation

siRNA resistance to siRNA#2 with 4 silent mutatio…

Promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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1127-DP NTP wild type

Plasmid

#32398

Depositor

Kathleen Green

Article

Kowalczyk et al J Cell Biol. 1997 Nov 3;139(3):773-84.

Insert

Desmoplakin (DSP Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Contains only the first 584 aa

Promoter

CMV

Available since

Nov. 29, 2011

Availability

Academic Institutions and Nonprofits only
pMRE164

Plasmid

#118512

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mOrange2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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15x-QUAS-dTomato-T2A-TRPV1

Plasmid

#140945

Purpose

QUAS reporter line that expresses cytosolic dTomato and the rat TRPV1 ion channel, separated by T2A

Depositor

Leslie Vosshall

Article

Jove et al Neuron. 2020 Dec 23;108(6):1163-1180.e12

Insert

15x-QUAS-dTomato-T2A-TRPV1

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCLV3Cas-LT1

Plasmid

#210549

Purpose

Binary vector expressing the Lineage Tracing system. Cas9 expression driven by CLV3 promoter. LT1 reporter

Depositor

Ortrun Mittelsten Scheid

Article

Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

Insert

CLV3p::spCas9::PEA3At::U6p::AAVS1gRNA::pHTR5::LT1::mCherrySSM

Use

Tags

Expression

Plant

Mutation

2bp insertion after AAVS1 target sequence that di…

Promoter

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSB-CMV-MCS-puro PM-mKate2-P2A-GEM-LTB4mut

Plasmid

#202644

Purpose

Expresses the LTB4-insensitive control sensor GEM-LTB4mut along with the plasma membrane targeted mKate2 tag in mammalian cells

Depositor

Balázs Enyedi

Article

Tamas et al Nat Commun. 2023 Aug 1;14(1):4610. doi:

Insert

PM-mKate2-P2A-GEM-LTB4mut

Use

Tags

PM-mKate2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pGM1202

Plasmid

#69615

Purpose

inducible gene expression in Streptomyces, C-terminal His-tag encoding sequence

Depositor

Günther Muth

Article

Muth et al Appl Microbiol Biotechnol. 2018 Nov;102(

Inserts

aac
tsr
rep
sso
TipA promoter
dso

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pCB168

Plasmid

#37389

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

Insert

dynein cytoplasmic 1 intermediate chain 1 (DYNC1I1 Human)

Use

Retroviral

Tags

GFP, S-tag, and TEV

Expression

Mammalian

Mutation

Promoter

Available since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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E407V Nuclear Myosin I YFP

Plasmid

#60242

Purpose

Expresses a E407V mutant of the nuclear isoform of myosin IC attached to YFP and an NLS, which has impaired actin binding and motor activities

Depositor

Primal de Lanerolle

Article

Chuang et al Curr Biol. 2006 Apr 18;16(8):825-31.

Insert

Nuclear Myosin 1 (Myo1c Mouse)

Use

Tags

EYFP and NLS

Expression

Mammalian

Mutation

E407V

Promoter

Available since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only
pSYN-PACE-001

Plasmid

#42503

Depositor

Robert Peters

Article

Peters et al J Thromb Haemost. 2012 Dec 4. doi: 10.11

Insert

soluble PACE (FURIN Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

cmv

Available since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

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pSH231-EF1-Puro

Plasmid

#115143

Purpose

Safe harbor site 231 knock-in vector with puromycin resistance selection cassette

Depositor

Michael Phelps, Raymond Monnat

Article

Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

Insert

Puromycin

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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Emi LC

Plasmid

#113666

Purpose

For transient expression of Emicizumab sequence (CAS# 1610943-06-0, KEGG# D10821), IgG4 bispecific antibody targeting FIX(a) & FX(a). Must be co-expressed with anti-FIX(a) and anti-FX(a) Emi HC's.

Depositor

Robert Peters

Article

Leksa et al J Thromb Haemost. 2019 Mar 18. doi: 10.1

Insert

Emicizumab light chain

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV Promoter

Available since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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Jag1-HA pIRES

Plasmid

#17336

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2007 Dec 12. ():.

Insert

Jagged1 (Jag1 Rat)

Use

Tags

HA X3

Expression

Mammalian

Mutation

A silent C->G mutation at 3237

Promoter

Available since

April 24, 2008

Availability

Academic Institutions and Nonprofits only

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pCB163

Plasmid

#37388

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

Insert

Dynactin 2 (DCTN2 Human)

Use

Retroviral

Tags

GFP, S-tag, and TEV

Expression

Mammalian

Mutation

A7P

Promoter

Available since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
mH2A1.2-CT-GFP

Plasmid

#45169

Depositor

Brian Chadwick, Hunt Willard

Article

Chadwick et al Hum Mol Genet. 2001 May 1;10(10):1101-13

Insert

H2Afy (MACROH2A1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

July 2, 2013

Availability

Academic Institutions and Nonprofits only

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pBC155

Plasmid

#202274

Purpose

Level 2 for strain-specific barcode DNA tag with bc182 through Tn7 bacterial insertion, Spectinomycin and Streptomycin selectable marker and tagBFP fluorescent marker

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

bc182

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pBC114

Plasmid

#202276

Purpose

Level 2 for strain-specific barcode DNA tag with bc141 through Tn7 bacterial insertion, Tetracyline selectable marker and far-red-FP fluorescent marker mPlum

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

bc141

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS human IRS-1

Plasmid

#11359

Depositor

Ronald Kahn

Article

Araki et al Diabetes. 1993 Jul . 42(7):1041-54.

Insert

IRS-1 (IRS1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 1, 2006

Availability

Academic Institutions and Nonprofits only

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p5E-hs_ICAM_full (JDW 1047)

Plasmid

#224479

Purpose

Gateway compatible 5' entry clone containing the Human ICAM2 promoter

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

ICAM2 promoter

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSYN-PC5-003

Plasmid

#42501

Depositor

Robert Peters

Article

Peters et al J Thromb Haemost. 2012 Dec 4. doi: 10.11

Insert

PC5 (PCSK5 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

cmv

Available since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
mH2A1.1-CT-MYC

Plasmid

#45166

Depositor

Brian Chadwick, Hunt Willard

Article

Chadwick et al Hum Mol Genet. 2001 May 1;10(10):1101-13

Insert

H2Afy (MACROH2A1 Human)

Use

Tags

HIS and MYC

Expression

Mammalian

Mutation

Promoter

Available since

July 2, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-Incenp wt

Plasmid

#108487

Purpose

expression of vsv-INCENP wt-mCherry

Depositor

Susanne Lens

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

INCENP (INCENP Human)

Use

Tags

VSV and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMDJ17 - pEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]

Plasmid

#159803

Purpose

Expression construct with ubiquitous expression to test fluorescence level on microscope

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-Myc CDC4 R465C

Plasmid

#16653

Depositor

Bert Vogelstein

Article

Rajagopalan et al Nature. 2004 Mar 4. 428(6978):77-81.

Insert

CDC4 R465C (FBXW7 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

R465C

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pAB078d8

Plasmid

#79206

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1+2) luxAB; Ppro1 434cI-SH2ABL1

Use

Tags

Expression

Bacterial

Mutation

Promoter

PlacZ-opt (OR1+2); Ppro1

Available since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEM p21

Plasmid

#8443

Depositor

Frederic Mushinski

Article

Huppi et al Oncogene 1994 Oct;9(10):3017-20.

Insert

Mouse p21 (Cdkn1a Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pAB076i5

Plasmid

#79199

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1+2+3) gIII, luxAB; Ppro1 434cI-SH2ABL1

Use

Tags

Expression

Bacterial

Mutation

Promoter

PlacZ-opt (OR1+2+3); Ppro1

Available since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCFJ2440 - [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), no_atg, no stop)

Plasmid

#159849

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), no_atg, no stop)

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAB078d3

Plasmid

#79203

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1) luxAB; Ppro1 434cI-SH2ABL1

Use

Tags

Expression

Bacterial

Mutation

Promoter

PlacZ-opt (OR1); Ppro1

Available since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRE141

Plasmid

#118493

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mTurquoise2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pQCascade

Plasmid

#140622

Purpose

Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and CRISPR RNA

Depositor

Sheng Yang

Article

Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

Insert

VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(entry)

Use

CRISPR; Transposon

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
p5078 pcDNA4c hBrd4 (1-470)

Plasmid

#14454

Depositor

Peter Howley

Article

You et al Cell. 2004 Apr 30. 117(3):349-60.

Insert

Brd4 (1-470) (BRD4 Human)

Use

Tags

Expression

Mammalian

Mutation

amino acids 1-470

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
NLS pEGFP-N3 (1299)

Plasmid

#62043

Purpose

mammalian expression of NLS-EGFP

Depositor

Eric Schirmer

Article

Zuleger et al Genome Biol. 2013 Feb 15;14(2):R14. doi:

Insert

SV40 NLS

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only
pAdEasy-Msln-iCre-HA-Flag

Plasmid

#31305

Depositor

William Pu

Article

Zhou et al J Clin Invest. 2011 May 2. 121(5):1894-9

Insert

Mesothelin (MSLN Human)

Use

Adenoviral

Tags

Flag and HA

Expression

Mutation

Promoter

Available since

March 1, 2012

Availability

Academic Institutions and Nonprofits only
sg14x(MS2) MUC4.1

Plasmid

#101153

Purpose

gRNA with 14 MCP binding site

Depositor

Mazhar Adli

Article

Qin et al Nat Commun. 2017 Mar 14;8:14725. doi: 10

Insert

MUC4 (MUC4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge

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