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Addgene

We narrowed to 8,867 results for: BLI;

Showing: 2801 - 2850 of 8867 results
  1. ecALDH

    Plasmid
    #67921
    Purpose
    Expresses aldehyde dehydrogenase from Escherichia coli, codon optimized for expression in E. coli
    Depositor
    Insert
    Escherichia coli aldehyde dehydrogenase (puuC Escherichia coli)
    Use
    Tags
    6x-His
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Sept. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
  2. pT7-V5-SBP-C1-HshnRNPE1

    Plasmid
    #64921
    Purpose
    Expresses V5- and SBP-tagged hnRNPE1 in mammalian cells
    Depositor
    Insert
    hnRNPE1 (PCBP1 Human)
    Use
    Tags
    V5 and SBP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 29, 2015
    Availability
    Academic Institutions and Nonprofits only
  3. pCEV-G2-Km ymScarletI

    Plasmid
    #193960
    Purpose
    TEF1 promoter controlled expression plasmid with G418 resistance for expressing ymScarletI in budding yeast
    Depositor
    Insert
    ymScarletI
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF1
    Available since
    Feb. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
  4. pJJR81

    Plasmid
    #75029
    Purpose
    tagBFP2^SEC^3xFlag vector with ccdB markers for cloning homology arms
    Depositor
    Insert
    tagBFP2^SEC^3xFlag
    Use
    CRISPR
    Tags
    3xFLAG and C. elegans codon optimized tagBFP2
    Expression
    Worm
    Mutation
    Promoter
    Available since
    June 21, 2016
    Availability
    Academic Institutions and Nonprofits only
  5. pLIX403-EBFP2-ccdB-Puro

    Plasmid
    #158552
    Purpose
    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions
    Depositor
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    EBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
  6. pFastBacMam6_C_3C_His8_Stop

    Plasmid
    #178810
    Purpose
    Baculovirus gene transfer into Mammalian cells to product recombinant proteins.
    Depositor
    Type
    Empty backbone
    Use
    Tags
    3C, His8, Stop codon
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
  7. pET-29b(+)_NSP4ss_SerM_NucA-myc

    Plasmid
    #218191
    Purpose
    Bacterial expression of a periplasmically expressed myc-tagged Serratia Marcescens NucA (Benzonase)
    Depositor
    Insert
    nucA
    Use
    Tags
    NSP4 signal secretion peptide and myc
    Expression
    Bacterial
    Mutation
    codon optimized ecoli residues - 22-266
    Promoter
    T7
    Available since
    May 17, 2024
    Availability
    Academic Institutions and Nonprofits only
  8. pET3a-His-DJ1

    Plasmid
    #51488
    Purpose
    Inducible expression of human His-DJ1 in E.coli
    Insert
    DJ1 (PARK7 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 31, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
  9. pLV-NT-3-mCherry

    Plasmid
    #190201
    Purpose
    To express the mouse NT-3 tagged with the FP mCherry
    Depositor
    Insert
    NT-3
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    The codon were optimized for better expression
    Promoter
    CMV
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
  10. pnos-Fok1:dCas9-nos

    Plasmid
    #62210
    Purpose
    Expresses Fok1:dCas9 under control of nanos promoter and 3'UTR. For germ line restricted high specificity genome engineering in Drosophila melanogaster.
    Depositor
    Insert
    Fok1-dCas9
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    nanos
    Available since
    March 12, 2015
    Availability
    Academic Institutions and Nonprofits only
  11. TRIOBP-TwinStrepII

    Plasmid
    #83201
    Purpose
    Expression of tagged protein in mammalian cells
    Insert
    TRIOBP (Triobp Mouse)
    Use
    Tags
    Twin Strep Tag II
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
  12. pAc5.1B-EGFP-DmRRP40

    Plasmid
    #65797
    Purpose
    expresses EGFP-tagged RRP40 in Drosophila cells
    Depositor
    Insert
    RRP40 (Rrp40 Fly)
    Use
    Drosophila expression
    Tags
    EGFP
    Expression
    Mutation
    Promoter
    Actin 5C
    Available since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
  13. pLenti-ePEG-LINE1-loxPsym

    Plasmid
    #216866
    Purpose
    Lentiviral guide RNA vector to insert the loxPsym sequence into LINE1 elements
    Depositor
    Insert
    spacerLINE1_cr772scaffold_PBS-loxPsm-HA-tevopreQ
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    U6
    Available since
    Aug. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
  14. pLIX403-EBFP2-ccdB-G418

    Plasmid
    #158556
    Purpose
    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions
    Depositor
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    EBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
  15. MIG-REST

    Plasmid
    #91898
    Purpose
    Expression of mouse REST
    Depositor
    Insert
    REST (Rest Mouse)
    Use
    Retroviral
    Tags
    ires-GFP
    Expression
    Mammalian
    Mutation
    Promoter
    MSCV
    Available since
    June 26, 2017
    Availability
    Academic Institutions and Nonprofits only
  16. mouse ptc probe M2-3

    Plasmid
    #58701
    Purpose
    makes mouse ptc probe for in situ hybridization
    Depositor
    Insert
    ptc (Ptch1 Mouse)
    Use
    In situ probe
    Tags
    Expression
    Bacterial
    Mutation
    only EcoRI fragment (5' end)
    Promoter
    T7
    Available since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
  17. pKSB- 3xP3-mTurqSV40 GGGG AAGA

    Plasmid
    #174533
    Purpose
    3xP3-mTurquoise fluorescence marker cassette for gene knock-in in mosquitoes
    Depositor
    Insert
    3xP3-mTurquoise BsaI cassette
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    3xP3
    Available since
    Sept. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
  18. pJM104: pTrc RFP on pCM66T

    Plasmid
    #74745
    Purpose
    pCM66T with promoter pTrc driving RFP expression (maxRFP = BBa_E1010)
    Depositor
    Insert
    RFP (BioBrick part number BBa_E1010)
    Use
    Tags
    Expression
    Mutation
    Promoter
    pTrc
    Available since
    May 16, 2016
    Availability
    Academic Institutions and Nonprofits only
  19. Sperm whale Mb 64Y68F in pVP80K

    Plasmid
    #86956
    Purpose
    Increases expression of sperm whale myoglobin protein for making apomyoglobin as a reagent for measuring rates of hemin dissociation (as opposed to previous construct in pUC).
    Depositor
    Insert
    Mb 64Y68F
    Use
    Tags
    Expression
    Bacterial
    Mutation
    64Y68F
    Promoter
    T5
    Available since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
  20. pB-CAG-PE2-NatMX

    Plasmid
    #158567
    Purpose
    Piggybac vector that allows for the generation of PE2-expressing stable cell lines
    Depositor
    Insert
    PE2
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
  21. pKT-PDGFRa-IRES-Katushka

    Plasmid
    #133081
    Purpose
    Expresses PDGFRa receptor and katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.
    Depositor
    Insert
    PDGFRa (Pdgfra Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
  22. pLIX403-EBFP2-ccdB-Blast

    Plasmid
    #158557
    Purpose
    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions
    Depositor
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    EBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
  23. pJK615

    Plasmid
    #71699
    Purpose
    Produces E. coli thioredoxin with a C-terminal His6 tag (EcTrxAH6)
    Depositor
    Insert
    thioredoxin
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
  24. pLXIN2-RFP

    Plasmid
    #99205
    Purpose
    RFP expressing bicistronic retroviral vector
    Depositor
    Insert
    Red fluorescent protein
    Use
    Retroviral; Bicistronic retroviral expression vec…
    Tags
    Expression
    Mammalian
    Mutation
    The dsRed cDNA was PCR amplified off AddGene plas…
    Promoter
    Available since
    Aug. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
  25. pUA97

    Plasmid
    #69340
    Purpose
    It encodes a fluorescent protein that can be fused to a gene of interest using SEC CRISPR strategy
    Depositor
    Insert
    ceBFP SEC 3xFlag
    Use
    Tags
    3xFlag and ceBFP (mTagBFP)
    Expression
    Worm
    Mutation
    sqt-1(e1350)
    Promoter
    Available since
    Dec. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
  26. pExp-PpiB

    Plasmid
    #112572
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and PpiB (peptidyl-prolyl cis-trans isomerase B from E. coli) fusions with optional C-terminal StrepII-tag
    Depositor
    Type
    Empty backbone
    Use
    Tags
    8xHis-PpiB and Strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
  27. pHH0103 NEDD4 WW domain #3

    Plasmid
    #104210
    Purpose
    Bacterial expression of WW domain #3 from NEDD4
    Insert
    NEDD4 WW domain #3 (NEDD4 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
  28. pHH0103 NEDD4L WW domain #2

    Plasmid
    #104205
    Purpose
    Bacterial expression of WW domain #2 from NEDD4L
    Insert
    NEDD4L WW domain #2 (NEDD4L Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
  29. pGAL-flp(H305L)-NAT

    Plasmid
    #160380
    Purpose
    overexpression of Flp recombinase step-arrest mutant in yeast
    Depositor
    Insert
    FLP1
    Use
    Tags
    Expression
    Yeast
    Mutation
    H305L
    Promoter
    GAL
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
  30. pLinker-BirA-3xHA-HXGPRT-loxP

    Plasmid
    #86668
    Purpose
    for generation of BirA tagged strains using CRISPR tagging technology
    Depositor
    Insert
    Linker-BirA-3xHA-HXGPRT 3'UTR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
  31. pHH0103 NEDD4 WW domain #1

    Plasmid
    #104208
    Purpose
    Bacterial expression of WW domain #1 from NEDD4
    Insert
    NEDD4 WW domain #1 (NEDD4 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Feb. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
  32. AAVS1_Puro_H2BK_mNG_hSLBP(18-108)

    Plasmid
    #191529
    Purpose
    Donor for targeted integration of mNG_hSLBP(18-108) probe to the AAVS1 safe harbor locus
    Depositor
    Insert
    H2BK_mNG_hSLBP(18-108)
    Use
    CRISPR and Synthetic Biology
    Tags
    mNeonGreen (mNG)
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
  33. pDTA-5

    Plasmid
    #128845
    Purpose
    Gateway-compatible p15A origin plasmid with a DTA negative selection cassette for targeting vector construction, I-CeuI, low copy number
    Depositor
    Type
    Empty backbone
    Use
    Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
  34. pHH0103 NEDD4 WW domain #4

    Plasmid
    #104211
    Purpose
    Bacterial expression of WW domain #4 from NEDD4
    Insert
    NEDD4 WW domain #4 (NEDD4 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
  35. pJK633

    Plasmid
    #72397
    Purpose
    Produces Escherichia coli aminoimidazole ribonucleotide synthetase, N-terminal His6 tag (H6EcPurM)
    Depositor
    Insert
    phosphoribosylaminoimidazole synthetase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
  36. pKSB- 3xP3-GFPsv40 GGGG AAGA

    Plasmid
    #174531
    Purpose
    3xP3-GFP fluorescence marker cassette for gene knock-in in mosquitoes
    Depositor
    Insert
    3xP3-GFP BsaI cassette
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    3xP3
    Available since
    Sept. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
  37. PB CAG-sfGFP-iCre-ERT2

    Plasmid
    #137856
    Purpose
    CAG-sfGFP-iCre-ERT2 piggyBac transposon
    Depositor
    Insert
    sfGFP-iCre-ERT2
    Use
    Cre/Lox; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
  38. CbSBS-Flag/TRRAP(S722F)

    Plasmid
    #32105
    Depositor
    Insert
    Transformation/transcription domain-associated protein (S722F) (TRRAP Human)
    Use
    Tags
    Flag 1x
    Expression
    Mammalian
    Mutation
    Changes serine 722 to Phe
    Promoter
    CMV
    Available since
    Nov. 14, 2011
    Availability
    Academic Institutions and Nonprofits only
  39. Preprotachykinin A

    Plasmid
    #130664
    Purpose
    Expresses human Preprotachykinin A in mammalian cells
    Depositor
    Insert
    Preprotachykinin A (TAC1 Human)
    Use
    Tags
    Fusion Red
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
  40. pcDNA3.1 myc-PITPalpha

    Plasmid
    #58277
    Purpose
    mammalian expression of human PITP alpha
    Depositor
    Insert
    PITP alpha (PITPNA Human)
    Use
    Tags
    myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 21, 2014
    Availability
    Academic Institutions and Nonprofits only
  41. pB6-ROSA26-DTA-Capecchi-for

    Plasmid
    #125743
    Purpose
    B6J ROSA26 targeting vector homology arms, Capecchi configuration, DTA, I-CeuI, forward cloning sites, low copy number
    Depositor
    Type
    Empty backbone
    Use
    Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
  42. pET21a(+)_C.jejuni m23 peptidase

    Plasmid
    #83297
    Purpose
    Expresses m23 peptidase from Campylobacter jejuni
    Depositor
    Insert
    ERS445056_01343
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    deleted amino acid 1-20
    Promoter
    Available since
    Nov. 3, 2016
    Availability
    Academic Institutions and Nonprofits only
  43. pNB3033

    Plasmid
    #113397
    Purpose
    Ty3 Gag3-His tagged, codon optimized for expression in E. coli; . IPTG inducible
    Depositor
    Insert
    Ty3 Gag3 codon optimized for E. coli expression
    Use
    Tags
    Gag3-6XHis tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 16, 2018
    Availability
    Academic Institutions and Nonprofits only
  44. 4IJD

    Plasmid
    #51328
    Depositor
    Insert
    4IJD (PRDM9 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Truncation: See Comments
    Promoter
    T7
    Available since
    Feb. 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
  45. pBS-L1PA1CH blast

    Plasmid
    #69612
    Purpose
    Expresses a blasticidin tagged codon optimized L1PA1
    Depositor
    Insert
    L1PA1
    Use
    Tags
    blast cassette
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
  46. p2Bac.Myc.p44

    Plasmid
    #39220
    Depositor
    Insert
    p44 (GTF2H2 )
    Use
    Tags
    Myc
    Expression
    Insect
    Mutation
    Promoter
    Pp10
    Available since
    Aug. 24, 2012
    Availability
    Academic Institutions and Nonprofits only
Showing: 2801 - 2850 of 8867 results