We narrowed to 2,461 results for: T2A

1. BCAM promoter-pRB-Puro-TurboRFPmyc-T2A-Luciferase

   Plasmid

   #188391

   Purpose

   Reporter for BCAM expression

   Depositor

   Markus Frank

   Article

   Sasamoto et al Cell Rep. 2022 Aug 9;40(6):111166. doi:

   Insert

   BCAM reporter
   

   Use

   Luciferase

   Available Since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. p155ME-loxP-dsR-loxP-Cas9-t2A-GFP

   Plasmid

   #227774

   Purpose

   Middle-entry vector with dsRed flanked by loxP sites and spCas9-GFP for Gateway cloning

   Depositor

   Bushra Raj

   Article

   Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

   Insert

   loxP-dsR-loxP
   

   Use

   CRISPR

   Available Since

   Dec. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pINDUCER-21-LCOR-P2A-HOXA9-T2A-HOXA5-IMPROVED

   Plasmid

   #154088

   Purpose

   Improved version to express LCOR, P2A, HOXA9, T2A and HOXA5 in mammalian cells.

   Depositor

   George Daley

   Article

   Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

   Insert

   Ligand Dependent Nuclear Receptor Corepressor, HOXA9, HOXA5 (LCOR, HOXA9, HOXA5 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   Tet on

   Available Since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. PCDNA3-FlipYFP(TEV cleavage seq) T2A mCherry

   Plasmid

   #124493

   Purpose

   Expresses FlipYFP (TEV cleavage sequence) and T2A mCherry

   Depositor

   Xiaokun Shu

   Article

   Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

   Insert

   FlipYFP(TEV cleavage seq) T2A mCherry
   

   Use

   Synthetic Biology

   Available Since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. P-Ef1a-RPL22-3XFlag-P2A-EGFP-T2A-Puro

   Plasmid

   #170318

   Purpose

   Expresses FLAG and EGFP tagged RPL22, puromycin resistance

   Depositor

   Hemali Phatnani

   Article

   Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

   Insert

   RPL22 (RPL22 Human)
   

   Use

   Lentiviral

   Mutation

   None

   Available Since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. P-Ef1a-RPL22-3XHA-P2A-EGFP-T2A-Neo

   Plasmid

   #170319

   Purpose

   Expresses HA and EGFP tagged RPL22, neomycin resistance

   Depositor

   Hemali Phatnani

   Article

   Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

   Insert

   RPL22 (RPL22 Human)
   

   Use

   Lentiviral

   Mutation

   None

   Available Since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pN1-CMV-Aff6_V18FdN-IntC-mCherryC-T2A-EGFP (C2)

   Plasmid

   #210725

   Purpose

   NIR optogenetic module for conditional protein splicing of split mCherry (C-part)

   Depositor

   Vladislav Verkhusha

   Article

   Karasev et al J Mol Biol. 2023 Dec 15;435(24):168360.

   Insert

   C2
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. P-Ef1a-RPL22-3XHA-P2A-EGFP-T2A-Puro

   Plasmid

   #170317

   Purpose

   Expresses HA and EGFP tagged RPL22, puromycin resistance

   Depositor

   Hemali Phatnani

   Article

   Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

   Insert

   RPL22 (RPL22 Human)
   

   Use

   Lentiviral

   Mutation

   None

   Available Since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEx1-pEF-H2B-mCherry-T2A-rTetR-KRAB

   Plasmid

   #78348

   Purpose

   Expresses rTetR-KRAB for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.

   Depositor

   Michael Elowitz

   Article

   Bintu et al Science. 2016 Feb 12;351(6274):720-4. do

   Insert

   H2B-mCherry-T2A-rTetR-KRAB
   

   Use

   Synthetic Biology

   Tags

   rTetR

   Expression

   Mammalian

   Promoter

   pEF alpha

   Available Since

   June 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. P-Ef1a-RPL22-3XHA-P2A-mCherry-T2A-Puro

    Plasmid

    #170320

    Purpose

    Expresses HA and mCherry tagged RPL22, puromycin resistance

    Depositor

    Hemali Phatnani

    Article

    Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

    Insert

    RPL22 (RPL22 Human)
    

    Use

    Lentiviral

    Mutation

    None

    Available Since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHD-T2A-split-intein-Gal4[21-881]-C-int

    Plasmid

    #199204

    Purpose

    To create "long homology arm" (~1000bp) knock-in donor vectors for split-intein Gal4[21-881]C-int

    Depositor

    Norbert Perrimon

    Article

    Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

    Insert

    T2A-split-intein-Gal4[21-881]-C-int
    

    Expression

    Insect

    Available Since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. P-Ef1a-RPL22-3XFlag-P2A-mCherry-T2A-Puro

    Plasmid

    #170322

    Purpose

    Expresses FLAG and mCherry tagged RPL22, puromycin resistance

    Depositor

    Hemali Phatnani

    Article

    Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

    Insert

    RPL22 (RPL22 Human)
    

    Use

    Lentiviral

    Mutation

    None

    Available Since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFH2.101_PB-EPN11-MCP-T2A-eGFP-PGK-Blast

    Plasmid

    #205546

    Purpose

    Express EPN11-MCP-T2A-eGFP and blasticidin resistance marker

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    EPN11-MCP
    

    Use

    Piggybac

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available Since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV[Exp]-Puro-EF-1α>TurboRFP(ns):T2A:{H2B-RFP}

    Plasmid

    #201120

    Purpose

    RFP expression in cells under EF-1α promoter; Histone-bound, nuclear

    Depositor

    Ori Bar-Nur

    Article

    Zvick et al Stem Cell Reports. 2022 Sep 13;17(9):194

    Insert

    TurboRFP; H2B-RFP
    

    Use

    Lentiviral

    Mutation

    No

    Available Since

    May 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. P-Ef1a-RPL22-3XHA-P2A-mCherry-T2A-Neo

    Plasmid

    #170321

    Purpose

    Expresses HA and mCherry tagged RPL22, neomycin resistance

    Depositor

    Hemali Phatnani

    Article

    Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

    Insert

    RPL22 (RPL22 Human)
    

    Use

    Lentiviral

    Mutation

    None

    Available Since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEx1-pEF-H2B-mCherry-T2A-rTetR-EED

    Plasmid

    #78101

    Purpose

    Expresses rTetR-EED for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.

    Depositor

    Michael Elowitz

    Article

    Bintu et al Science. 2016 Feb 12;351(6274):720-4. do

    Insert

    H2B-mCherry-T2A-rTetR-EED (EED Human, Synthetic)
    

    Use

    Synthetic Biology

    Tags

    rTetR

    Expression

    Mammalian

    Promoter

    pEF alpha

    Available Since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pU6-(BbsI)_CBh-Cas9-T2A-mCherry-H1-(BamHI)

    Plasmid

    #64217

    Purpose

    Expression vector for sgRNAs cloned into the BbsI sites, shRNAs into BamHI & AflII and for Expression of Cas9 linked to mCherry via T2A

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Inserts

    Cas9
    hU6 promoter; BbsI sites for sgRNA
    H1 promoter; BamHI site for shRNA
    

    Use

    CRISPR

    Tags

    3xFLAG, NLS, and T2A-mCherry

    Expression

    Mammalian

    Promoter

    CBh, H1, and U6

    Available Since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHAGE2-TetOminiCMV-Sox2-17-t2a-Klf4-e2a-Myc

    Plasmid

    #193349

    Purpose

    tetO-S*KM (Dox-inducible reprogramming lentiviral vector expressing mouse super-Sox + Klf4 + cMyc)

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    Sox2-17-t2a-Klf4-e2a-cMyc (Klf4, Sox2 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Sox2-17 is engineered highly cooperative chimeric…

    Promoter

    TetO mini CMV

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pIHEU-FlipGFP(Casp3 cleavage seq) T2A mCherry

    Plasmid

    #124434

    Purpose

    Expresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in Drosophila

    Depositor

    Xiaokun Shu

    Article

    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

    Insert

    FlipGFP(Casp3 cleavage seq) T2A mCherry
    

    Expression

    Insect

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTBL716 4xHRE-YB-TATA-Cas9-ODD-T2A-TdT

    Plasmid

    #132667

    Purpose

    Expresses hypoxia-inducible Cas9 and TdT in mammalian cells.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Insert

    Cas9-ODD-T2A-TdT (DNTT Human, Streptococcus pyogenes)
    

    Expression

    Mammalian

    Promoter

    4xHRE_YB TATA

    Available Since

    Nov. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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