We narrowed to 1,328 results for: piggyBac

1. pSLPB2B-ecDHFR-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

   Plasmid

   #187950

   Purpose

   ecDHFR degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

   Depositor

   Edda Schulz

   Article

   Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

   Insert

   ecDHFR-SpdCas9-tagRFPt-P2A-tagBFP
   

   Use

   CRISPR and Synthetic Biology

   Tags

   GGS linker

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-Neo-PuroDTK

   Plasmid

   #84036

   Purpose

   PiggyBac cassette contains Neomycin, puromycin and TK genes

   Depositor

   Yuet Wai Kan

   Article

   Ye et al Proc Natl Acad Sci U S A. 2014 Jul 1;111

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CAG promoter

   Available Since

   April 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB-CAG-DDdCas9VPPH-T2A-GFP-IRES-Neo

   Plasmid

   #102888

   Purpose

   PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-P300core-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   DDdCas9VP192-p65-HSF1-P300-T2A-GFP-IRES-Neo
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   CAG

   Available Since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSLPB2B-ER50-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

   Plasmid

   #187948

   Purpose

   ER50 degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

   Depositor

   Edda Schulz

   Article

   Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

   Insert

   ER50-SpdCas9-tagRFPt-P2A-tagBFP
   

   Use

   CRISPR and Synthetic Biology

   Tags

   GGS linker

   Expression

   Mammalian

   Mutation

   6 mutations, T371A, L384M, M421G, G521R, Y537S, N…

   Promoter

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pScarlessHD-N-3xVHH05-DsRed

   Plasmid

   #171581

   Purpose

   For scarless insertion of 3xVHH05 NanoTag into N-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

   Depositor

   Norbert Perrimon, Hidde Ploegh

   Article

   Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

   Insert

   3xVHH05 (UBE2J1 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-Ef1a-RPS27A-UTR-PP7-WPRE

   Plasmid

   #198339

   Purpose

   PB plasmid expressing RPS27A with its 3'UTR tagged to PP7 stem loops

   Depositor

   Michael Ward

   Article

   De Pace et al Nat Neurosci. 2024 Apr 10. doi: 10.1038/

   Insert

   RPS27A-3'UTR-PP7 stem loops x24
   

   Use

   Piggybac

   Tags

   PP7 stem loops

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available Since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. FLAG-FullLength-TET1-CXXC(24)

   Plasmid

   #124398

   Purpose

   Full-length TET1 missing zinc finger domain (585-624) used to PiggyBAC rescue the function of TET1/2 DKO cells

   Depositor

   Shaun Cowley

   Article

   Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

   Insert

   FLAG-FullLength-TET1 with CXXC zinc finger deletion (585-624) (TET1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   CXXC zinc finger deletion (aa 585-624)

   Promoter

   Available Since

   May 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSLQ2842 pPB: CAG-GID1-VPR-IRES-Zeo-WPRE PGK-GAI-tagBFP-SadCas9

   Plasmid

   #84244

   Purpose

   Expresses GA-inducible VPR-Sa dCas9

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Inserts

   GAI-tagBFP-Sa dCas9
   GID1-VPR
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   GAI-tagBFP-Nucleoplasmin NLS, GID1, IRES-Zeocin, …

   Expression

   Mammalian

   Mutation

   D10A, N580A and Synonymous mutation in PYL1 to re…

   Promoter

   CAG and PGK

   Available Since

   Nov. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSLPB2B-FKBP12_F36V-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

   Plasmid

   #187951

   Purpose

   FKBP12 (F36V mutant) degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

   Depositor

   Edda Schulz

   Article

   Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

   Insert

   FKBP12_F36V-SpdCas9-tagRFPt-P2A-tagBFP
   

   Use

   CRISPR and Synthetic Biology

   Tags

   GGS linker

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. BII-ChBtW-dnTCF4-ires-GFP

    Plasmid

    #133378

    Purpose

    Piggybac vector for constitutive expression

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    dnTCF4 (TCF4 Human)
    

    Use

    Tags

    2x Flag

    Expression

    Mammalian

    Mutation

    deltaN31

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. iMAP-61

    Plasmid

    #187460

    Purpose

    gRNA array of iMAP-61

    Depositor

    Tian Chi

    Article

    Liu et al Cell. 2022 Aug 4;185(16):3008-3024.e16.

    Insert

    sgRNA array
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    modified U6

    Available Since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pScarlessHD-N-3x127D01-DsRed

    Plasmid

    #171579

    Purpose

    For scarless insertion of 3x127D01 NanoTag into N-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

    Depositor

    Norbert Perrimon, Hidde Ploegh

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    3x127D01 (CXCR2 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPB-cT3G-cERP2-TSC22D3

    Plasmid

    #192916

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of TSC22D3

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    TSC22D3 (TSC22D3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    TET3G-miniCMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. XLone-Puro Cas13d-eGFP U6 SOX17 g2

    Plasmid

    #155188

    Purpose

    PiggybacTransposon-based tunable and temporal expression control of Cas13d- eGFP and SOX17 gRNA2

    Depositor

    Xiaoping Bao

    Article

    Chang et al Biomaterials. 2022 May 6;285:121569. doi

    Insert

    Cas13d SOX17 gRNA2
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSLQ2821 pPB: CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9-ABI

    Plasmid

    #84257

    Purpose

    Expresses Sp dCas9 and GA-inducible VPR for OR gate/diametric regulation

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    GAI-tagBFP-Sp dCas9-ABI
    GID1-VPR
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    2xNLS (SV40), ABI, GAI, GID1, HA tag, IRES-Puro, …

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and PGK

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB-Ef1a-SunTag-RPS7-PP7-WPRE

    Plasmid

    #198338

    Purpose

    PB plasmid expressing RPS7 mRNA tagged with SunTag repeats and PP7 stem loops

    Depositor

    Michael Ward

    Article

    De Pace et al Nat Neurosci. 2024 Apr 10. doi: 10.1038/

    Insert

    GCN4x24-RPS7-3'UTR-PP7 stem loops x24
    

    Use

    Piggybac

    Tags

    PP7 stem loops and SunTag

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. OA-1067A1

    Plasmid

    #164847

    Purpose

    Expresses four gRNA targeting BTub gene with 3xp3-eGFP marker

    Depositor

    Omar Akbari

    Article

    Li et al Nat Commun. 2021 Sep 10;12(1):5374. doi:

    Insert

    4 gRNAs and 3xP3-eGFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEF1a_BbsI-gRNA scaffold-EC73-5'-EcoRI-EC73-3'-HDV_pA_pCMV_EGFP-L202S-P2A-mCherry_pA

    Plasmid

    #176461

    Purpose

    Vector for constructing retron Ec73ncRNA-gRNA chimeric RNA (Ec73 rgRNA), inserting spacer sequence by BbsI digestion and inserting donor sequence by EcoRI digestion. EGFP(L202S)-P2A-mCherry driven by CMV

    Depositor

    Hui Yang

    Article

    Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/

    Insert

    BbsI-gRNA scaffold-Ec73ncRNA-EcoRI-HDV
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. FLAG-FullLength-TET1-LLAA(20)

    Plasmid

    #124397

    Purpose

    Full-length mutant TET1 (L897A/L900A) used to PiggyBAC rescue the function of TET1/2 DKO cells

    Depositor

    Shaun Cowley

    Article

    Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

    Insert

    FLAG-FullLength-TET1 with L897A/L900A mutations (TET1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    L897A/L900A mutations (full length protein is 213…

    Promoter

    Available Since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSLQ2818_Blasti_pPB: CAG-PYL1-KRAB-IRES-Blasti-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9-FKBP_F36V

    Plasmid

    #187959

    Purpose

    Expressed ABA-inducible dimerizing KRAB-dCas9 system, with KRAB-IRES-Blasticidin resistance under CAG promoter and tagBFP-dCas9-FKBP12 (F36V mutant) degron under PGK promoter in a piggyBac plasmid.

    Depositor

    Edda Schulz

    Article

    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

    Inserts

    Blasticidin resistance
    FKBP12_F36V
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GGS linker

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge