176,161 results

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pSLQ10875

Plasmid

#183963

Purpose

CBA-hyperdCas12a-HA-KRAB-mCherry

Depositor

Stanley Qi

Publication

Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

Insert

HyperdCas12a

Use

CRISPR

Tags

HA, KRAB, and mCherry

Expression

Mammalian

Mutation

D832A, D156R, E292R, D235R, D350R

Promoter

CBA

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV.hSyn.iGluSnFR3.v857.PDGFR

Plasmid

#178329

Purpose

Fluorescent reporter for glutamate, third generation, variant 857. iGluSnFR3.v857

Depositor

Kaspar Podgorski

Publication

Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

Has service

AAV1

Insert

pAAV hSyn iGluSnFR3 v857.PDGFR

Use

AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

Tags

IgK-chain, Myc epi tag, and PDGFR TM Domain

Expression

Mammalian

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pGL4.10/RSV_SF3B3-Xon_Luciferase

Plasmid

#174659

Purpose

Plasmid containing the SF3B3-Xon/Firefly luciferase cassette. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-xon by LMI070.

Depositor

Beverly L. Davidson

Publication

Monteys et al Nature. 2021 Jul 28. pii: 10.1038/s41586

Insert

Xon cassette

Use

Luciferase

Expression

Mammalian

Mutation

Kozak ATG initiation sequence in middle exon

Promoter

RSV

Available since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mBeRFP

Plasmid

#175173

Purpose

large-stokes shift red fluorescent protein mBeRFP

Depositor

Bernardo Sabatini

Publication

Melzer et al Cell. 2021 Oct 28;184(22):5622-5634.e25.

Insert

mBeRFP

Available since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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eGFP-KIF5B

Plasmid

#172203

Purpose

Expresses KIF5B tagged with eGFP in mammalian cells

Depositor

Juan Bonifacino

Publication

Guardia et al Cell Rep. 2016 Nov 15;17(8):1950-1961. d

Insert

KIF5B (Kif5b Mouse)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available since

Nov. 8, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRPN265NB-LaG16-MNase

Plasmid

#170978

Purpose

Expresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & Run

Depositor

Marc Timmers

Publication

Koidl et al Curr Protoc. 2021 Oct;1(10):e266. doi: 1

Insert

Glutathione-S-Transferase/LaG16/MNase

Tags

GST

Expression

Bacterial

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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bact2-mKate2-P2A-TurboID-dVHH[GFP]

Plasmid

#163857

Purpose

Zebrafish ubiquitous specific expression of mKate2-P2A-TurboID-dVHH[GFP]. Parton lab clone JBH

Depositor

Rob Parton

Publication

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

TurboID-VHH[EGFP]

Use

Zebrafish expression

Tags

mKate2-P2A-TurboID

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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H2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen

Plasmid

#129631

Purpose

Visualization of Akt and ERK activity

Depositor

Dorus Gadella

Publication

Chavez-Abiega et al J Cell Sci. 2022 Mar 15;135(6):jcs259685

Insert

H2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen

Expression

Mammalian

Promoter

CMV

Available since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pRU294

Plasmid

#167689

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Publication

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pEC1.2::Cas9i

Use

CRISPR

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3.3_MERS_D12

Plasmid

#170448

Purpose

Express MERS WT spike protein with an 12aa deletion on the C-terminal tail. Used for MERS pseudotyped virus production.

Depositor

David Nemazee

Publication

Rogers et al Science. 2020 Aug 21;369(6506):956-963.

Insert

MERS spike D12 (S MERS)

Expression

Mammalian

Mutation

last 12aa deletion in c-terminal tail

Promoter

CMV

Available since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pDONRp5-p2 GFP-NShroom3-iLID

Plasmid

#170976

Purpose

N-terminal component of OptoShroom3 optogenetic tool. Binds to apical junctions. pDONRp5-p2 plasmid.

Depositor

Miki Ebisuya

Publication

Martinez-Ara et al Nat Commun. 2022 Sep 14;13(1):5400. doi:

Insert

NShroom3 (Shroom3 Mouse)

Use

Gateway Cloning

Tags

eGFP and iLID

Mutation

From isoform 2, deleted amino acids 1389 - 1805,…

Available since

July 15, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-Sst44-GFP

Plasmid

#172310

Purpose

SST interneuron-restricted gene regulatory element GRE44, to drive GFP expression in SST+ interneurons

Depositor

Michael Greenberg

Publication

Hrvatin et al Elife. 2019 Sep 23;8. pii: 48089. doi: 1

Insert

Sst44

Use

AAV

Promoter

pB-Globin

Available since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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GfaABC1D BARK p2A mCherry

Plasmid

#117691

Purpose

GfaABC1D-iBARK-p2A-mCherry: Viral expression vector for astrocyte Gaq silencing

Depositor

Baljit Khakh

Publication

Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

Insert

BARKrgs peptide

Use

AAV

Tags

HA / FLAG

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV_hSynapsin_psychLight2

Plasmid

#163909

Purpose

To generate Adeno-Associated viruses for expression of psychLight2 under synapsin promoter

Depositor

Lin Tian

Publication

Dong et al Cell. 2021 Apr 8. pii: S0092-8674(21)003

Has service

AAV9

Insert

psychLight2

Use

AAV

Tags

Flag tag

Expression

Mammalian

Promoter

Synapsin

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCDH-3xFLAG-GFP-puroR

Plasmid

#167463

Purpose

3rd-gen lentiviral expression of FLAG-GFP in mammalian cells

Depositor

Steven Artandi

Publication

Chen et al Cell. 2018 Jun 28;174(1):218-230.e13. do

Insert

GFP

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pQE30-HyPer7

Plasmid

#141071

Purpose

Bacterial expression of ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

Depositor

Vsevolod Belousov

Publication

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Insert

HyPer7

Tags

Hisx6

Expression

Bacterial

Promoter

T5

Available since

March 1, 2021

Availability

Academic Institutions and Nonprofits only

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pTRE3G-NlucP

Plasmid

#162595

Purpose

C-terminally PEST-tagged NanoLuc under TRE3G promoter

Depositor

Masaharu Somiya

Publication

Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

Insert

NanoLuc-PEST

Tags

PEST

Expression

Mammalian

Promoter

TRE3G promoter

Available since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJT126 lenti pEF-rTetR(SE-G72P)-3XFLAG-LibCloneSite-T2A-mCherry-BSD-WPRE

Plasmid

#161926

Purpose

Lentiviral backbone for GoldenGate cloning effectors as a fusion with rTetR(SE-G72P)-3XFLAG

Depositor

Michael Bassik, Lacra Bintu

Publication

Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315

Insert

rTetR(SE-G72P)

Use

Lentiviral and Synthetic Biology

Tags

3XFLAG

Expression

Mammalian

Promoter

pEF

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pSL21-mCherry

Plasmid

#164410

Purpose

Retroviral expression plasmid of sgRNA with mCherry

Depositor

Junwei Shi

Publication

Chen et al Cell. 2021 Mar 4;184(5):1262-1280.e22. d

Type

Empty backbone

Use

CRISPR and Retroviral

Expression

Mammalian

Promoter

U6 promoter for crRNA expression and EFS promoter…

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pAAV.GFAP.(Cyto)iGlucoSnFR

Plasmid

#164513

Purpose

Green glucose sensor, cytosolic. The depositing lab recommends the updated pAAV.GFAP.(cyto).iGlucoSnFR2 (Plasmid #244111) instead of this plasmid.

Depositor

Loren Looger

Publication

Keller et al Cell Rep. 2021 Jun 22;35(12):109284. doi

Insert

(Cyto)iGlucoSnFR

Use

AAV

Mutation

Synthetic Construct

Promoter

GFAP

Available since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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