We narrowed to 21,714 results for: ESC;

1. pNKX2-5-T2A-GFP-PGK-Puro

   Plasmid

   #203607

   Purpose

   Donor plasmid to create 3' knockin of NKX2-5 with T2A-GFP

   Depositor

   Matthew Birket

   Article

   Douglas et al Stem Cell Res. 2023 Dec 3;74:103262. doi

   Insert

   NKX2-5-T2A-GFP-PGK-Puro-NKX2-5 (NKX2-5 Human)
   

   Use

   Human targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-gfaABC1D-HA-eLACCO2.1-NGR

   Plasmid

   #208098

   Purpose

   Biosensor for extracellular L-lactate

   Depositor

   Marie-Eve Paquet, Robert Campbell

   Article

   Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

   Insert

   R-iLACCO1
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. EFS-SpdCas9

   Plasmid

   #210703

   Purpose

   This plasmid express EFS promoter driven human codon optimized SpdCas9

   Depositor

   Isaac Hilton

   Article

   Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

   Insert

   S. Pyogenes dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D10A/H840A

   Promoter

   EF1a core promoter

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmiRFP2-Actin-C-18

   Plasmid

   #178965

   Purpose

   Expresses the fusion protein of miRFP2-Actin-C in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

   Insert

   miRFP2-actin
   

   Use

   Tags

   actin

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-GFP11(x7)-Actin

   Plasmid

   #181967

   Purpose

   Human beta actin tagged with 7 copies of GFP11.

   Depositor

   Jin Zhang

   Article

   Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

   Insert

   GFP11(x7)-Actin (ACTB )
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CaMKIIa-DIO eHcKCR1 3.0-oScarlett-KV2.1

   Plasmid

   #195198

   Purpose

   all-optical silencing method

   Depositor

   Karl Deisseroth

   Article

   Fan et al Cell. 2023 Feb 2;186(3):543-559.e19. doi

   Insert

   eHcKCR1 3.0-oScarlet-Kv2.1
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CaMKIIa

   Available since

   Feb. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. nuc-GafD-mTurboID-HA

   Plasmid

   #184641

   Purpose

   Expresses 3xHA-tagged GlycoID construct: GafD_short-miniTurboID in mammalian nucleus for O-GlcNAc interactome tagging

   Depositor

   Charlie Fehl

   Article

   Liu et al ACS Chem Biol. 2022 Jul 12. doi: 10.1021

   Insert

   nuc-GlycoID-HA
   

   Use

   Tags

   3xHA tag and Nuclear localization sequence

   Expression

   Mammalian

   Mutation

   GafD -> expressed 22-178 ; BirA* -> express…

   Promoter

   CMV

   Available since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAcBac1.tR4-AcdRS82

   Plasmid

   #174101

   Purpose

   Expression of orthogonal Mb tRNA and Acd82 tRNA synthetase for incorporation of acridone in eukaryotic cells.

   Depositor

   Ryan Mehl

   Article

   Jones et al Chem Sci. 2021 Aug 3;12(36):11955-11964.

   Insert

   M. barkeri tRNA synthetase for acridone82
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N311S, C313G, V366A, W382T

   Promoter

   CMV

   Available since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. CDK4-3XFlag-pBabe

   Plasmid

   #73558

   Purpose

   vector encoding a fusion protein between CDK4 and 3 Flag tags

   Depositor

   Gerardo Ferbeyre

   Article

   Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

   Insert

   CDK4 (CDK4 Human)
   

   Use

   Retroviral

   Tags

   3X-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAGM22082_cRed

    Plasmid

    #117225

    Purpose

    pET-28b backbone plasmid, Level 2 Golden Gate expression plasmid.

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Sci Rep. 2019 Jul 29;9(1):10932. doi: 10

    Insert

    Canthaxanthin Operon
    

    Use

    Tags

    34 amino acid N-terminal linker, Hexahistidine Ta…

    Expression

    Bacterial

    Mutation

    removed Type IIS restriction sites within the bac…

    Promoter

    T7 RNA Polymerase

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGreenFire_blastocidin

    Plasmid

    #174103

    Purpose

    pGreenFire MPRA plasmid for cloning

    Depositor

    Paul Khavari

    Article

    Guo et al Nat Genet. 2023 Nov;55(11):1876-1891. do

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. hHS1-M7A

    Plasmid

    #159170

    Purpose

    Human codon-optimized cytosolic labile heme reporter

    Depositor

    Amit Reddi

    Article

    Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

    Insert

    hHS1-M7A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Mutated Met 7 of the cyt b562 module of hHS1 to A…

    Promoter

    CMV

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-gfaABC1D-R-iLACCO1.2

    Plasmid

    #208104

    Purpose

    Biosensor for intracellular L-lactate

    Depositor

    Marie-Eve Paquet, Robert Campbell

    Article

    Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

    Insert

    R-iLACCO1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. SiriusGFP

    Plasmid

    #123201

    Purpose

    We have developed a variant of eGFP, SiriusGFP, that shows over a two fold increase in photostability with utility in methods requiring sustained or high intensity excitation as in 4D confocal or SIM.

    Depositor

    Joseph Santos-Sacchi

    Article

    Zhong et al J Neurosci Methods. 2019 Feb 1;313:68-76

    Insert

    SiriusGFP
    

    Use

    Mouse Targeting

    Tags

    N/A

    Expression

    Bacterial and Mammalian

    Mutation

    eGFP-S30R/Y39N/F99S/N105T/S147R/M153T/V163A/S205V…

    Promoter

    CMV

    Available since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-GalT-mCherry

    Plasmid

    #214271

    Purpose

    Mammalian expression of GalT-mCherry (Golgi marker)

    Depositor

    Shinya Tsukiji

    Article

    Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

    Insert

    GalT(1-82)-mCherry (B4GALT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAS_4xBstTyrT(CUA)_EcoTyrRS-FLAG

    Plasmid

    #140018

    Purpose

    Plasmid with 4xBstTyrT(CUA) cassette and E. coli TyrRS for amber suppression; for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    EcoTyrRS
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Y37L, D182S, F183M, L186A

    Promoter

    EF1

    Available since

    May 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. mOrange2-Mito-7

    Plasmid

    #57969

    Purpose

    Localization: Mitochondria, Excitation: 549, Emission: 565

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Insert

    Mito (COX8A Human)
    

    Use

    Tags

    mOrange2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

18. 5HT2C-RNA2-Flag

    Plasmid

    #79679

    Purpose

    serotonin receptor 2C isoform 2 with C-terminal Flag tag, non-edited isoform

    Depositor

    Stefan Stamm

    Article

    Zhang et al EMBO Mol Med. 2016 Aug 1;8(8):878-94. do

    Insert

    serotonin receptor 2C, RNA2 (HTR2C Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

19. TPC1-mCherry

    Plasmid

    #135182

    Purpose

    Expresses TPC1 with C-terminus mCherry tag in mammalian cells

    Depositor

    Antony Galione

    Article

    Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

    Insert

    TPC1 (Tpcn1 Mouse)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-hSyn-R-diLACCO1

    Plasmid

    #208102

    Purpose

    Control biosensor for intracellular L-lactate

    Depositor

    Marie-Eve Paquet, Robert Campbell

    Article

    Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

    Insert

    eLACCO2.1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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