We narrowed to 37,507 results for: Ran;

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pCAG-loxpSTOPloxp-lyn-NeonGreen

Plasmid

#175257

Purpose

membrane target protein labelling

Depositor

Frank Bradke, Sebastián Dupraz

Article

Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.37

Insert

Lyn membrane targeting motif

Use

Cre/Lox

Tags

mNeonGreen

Expression

Mammalian

Mutation

None (wt)

Promoter

pCAG

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pMXs-SOX10

Plasmid

#134669

Purpose

Retroviral expression of human SOX10

Depositor

Marius Wernig

Article

Tanabe et al Development. 2022 Oct 15;149(20). pii: 2

Insert

SRY-box transcription factor 10 (SOX10 Human)

Use

Retroviral

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pLV V5-LAP2beta

Plasmid

#175112

Purpose

Lentiviral expression of V5-tagged mouse Tmpo

Depositor

Larry Gerace

Article

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Tmpo (Tmpo Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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TVBB C-term-mNeongreen-Blast

Plasmid

#169229

Purpose

Targeting vector backbone to support a knock-in of Linker-mNeongreen-P2A-Blast at the C-terminus of a target locus

Depositor

Hugo Snippert

Article

Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

Insert

Double SapI flanked mNeongreen-P2A-Blast

Use

Targeting vector backbone

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pNH605-CCAAT-YFP

Plasmid

#172132

Purpose

Fluorescent reporter for HAP complex activity

Depositor

Johannes Herrmann

Article

Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

Insert

4xCCAAT-YFP

Use

Vector for genomic integration into the yeast gen…

Expression

Yeast

Promoter

CYC1 core promotor with 4 tandem repeats of the H…

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pH6HTC_ABCE1-Halo

Plasmid

#175335

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

ABCE1 (ABCE1 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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XLone-BSD Cas13d P2A mCherry

Plasmid

#155183

Purpose

PiggybacTransposon-based tunable and temporal expression control of Cas13d and mCherry

Depositor

Xiaoping Bao

Article

Chang et al Biomaterials. 2022 May 6;285:121569. doi

Insert

CasRx

Use

Unspecified; Piggybac transposon

Tags

NLS and P2A mCherry

Expression

Mammalian

Promoter

TRE3G

Available Since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-FOXN1

Plasmid

#101445

Purpose

Donor Vector containing FOXN1 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

FOXN1 (FOXN1 Human)

Use

Gateway Cloning

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pMDJ17 - pEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]

Plasmid

#159803

Purpose

Expression construct with ubiquitous expression to test fluorescence level on microscope

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pET-45b-RUNX2(WT)-IDR-mCherry

Plasmid

#145286

Purpose

Bacterial Expression of 6xHis RUNX2(WT) IDR mCherry Fusion Protein

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

RUNX2(WT)-IDR (RUNX2 Human)

Expression

Bacterial

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pGWB660-CYCD3;1

Plasmid

#140751

Purpose

Inducing plant cells to enter mitosis

Depositor

Bo Liu

Article

Xu et al New Phytol. 2019 Nov 2. doi: 10.1111/nph

Insert

Cyclin D3;1 (CYCD3;1 Mustard Weed)

Tags

TagRFP

Expression

Plant

Promoter

CaMV 35 promoter

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-hNme2Cas9-NLS(SV40)-3xFLAG (KAC582)

Plasmid

#134331

Purpose

CAG promoter expression plasmid for human codon optimized Nme2Cas9 nuclease with C-terminal NLS (SV40) and 3x FLAG tag

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized Nme2Cas9

Tags

NLS(SV40)-3xFLAG

Expression

Mammalian

Promoter

CAG

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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YFP-Sp100A(K297A)-C1

Plasmid

#134552

Purpose

Expresses YFP-Sp100A fusion protein with a K297A mutation

Depositor

Susan Janicki

Article

Newhart et al Mol Biol Cell. 2013 Mar 13.

Insert

Sp100A(K297A)

Tags

YFP

Expression

Mammalian

Mutation

K297R point mutation in the SUMO site

Promoter

CMV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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YFP-Sp100C(W655Q)-C3

Plasmid

#134556

Purpose

Expresses YFP-Sp100C fusion protein with a W655Q mutation

Depositor

Susan Janicki

Article

Newhart et al Mol Biol Cell. 2013 Mar 13.

Insert

Sp100C(W655Q)

Tags

YFP

Expression

Mammalian

Mutation

W655Q point mutation in the SAND domain

Promoter

CMV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a His6Flag2AUF1(p37)

Plasmid

#135939

Purpose

Expresses AUF1p37 in E. coli

Depositor

Yimon Aye

Article

Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

Insert

heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)

Tags

Flag2 and His6

Expression

Bacterial

Promoter

T7

Available Since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC22A18

Plasmid

#132047

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC22A18 (SLC22A18 Human)

Expression

Mammalian

Available Since

Nov. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pAPM-D4 miR30-SETDB1 ts1

Plasmid

#115859

Purpose

SETDB1 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SETDB1 shRNA (SETDB1 Human)

Use

Lentiviral

Promoter

SFFV

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pTAMAHISTEV_PfeA

Plasmid

#128943

Purpose

expresses PfeA in E. coli

Depositor

James Naismith

Article

Moynie et al Nat Commun. 2019 Aug 14;10(1):3673. doi:

Insert

ferric enterobactin receptor

Tags

TEV protease cleavable 7xHis and TamA

Expression

Bacterial

Mutation

Signal peptide sequence has been removed (1-75)

Promoter

T7

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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JL002: pMVP/eGFP-DEST

Plasmid

#121852

Purpose

pMVP destination vector, empty expression vector backbone w/ eGFP selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Pmvp gateway destination vector

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCS-CG-Lock-Cer-VWF

Plasmid

#124828

Purpose

Expresses multimeric human VWF-ECFP fusion protein with A2-Lock domain The cyan fluorescence protein coding region is inserted between the VWF-D'D3 and A1 domains.

Depositor

Sriram Neelamegham

Article

Zhang et al Blood Adv. 2019 Apr 9;3(7):957-968. doi:

Insert

VWF with cyan fluorescent protein fusion between D'D3 and A1 domains and Lock A2 domain (VWF Human)

Expression

Mammalian

Promoter

CMV

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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