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EZ-L767

Plasmid

#131773

Purpose

PADH1_sFUS_Fusion Red_PixD_TACT1

Depositor

Jared Toettcher

Article

Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

Insert

PADH1_sFUS_Fusion Red_PixD_TACT1

Use

Integration into δ-sites

Expression

Yeast

Promoter

PADH1

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCMB-PERr

Plasmid

#61203

Purpose

Fluorescent reporter for peroxisomes mCherry-SKL expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

mCherry-SKL

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available Since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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V957 pLPS-3' ECFP-mito - Acceptor

Plasmid

#11699

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

Type

Empty backbone

Use

Cre/Lox; Acceptor vector

Tags

ECFP-mito

Expression

Mammalian

Available Since

May 26, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pCMP-APr

Plasmid

#61184

Purpose

Fluorescent reporter for apoplast MtBCP1sp-mCherry expressed under MtPT4 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtBCPsp-mCherry

Tags

mCherry

Expression

Plant

Promoter

MtPT4

Available Since

April 23, 2015

Availability

Academic Institutions and Nonprofits only

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pRATIO2112

Plasmid

#141296

Purpose

Gateway cloning compatible dual fluorescent ratiometric binary vector

Depositor

David Nelson

Article

Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

Type

Empty backbone

Tags

mScarlet-I and mVenus

Expression

Plant

Promoter

UBQ10 promoter

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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Mis12 INCENP Δcen mCherry

Plasmid

#45228

Depositor

Michael Lampson, Susanne Lens

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

Mis12 INCENP Δcen mCherry

Tags

VSVG

Expression

Mammalian

Mutation

CEN box deleted from INCENP (aa1-46)

Available Since

July 16, 2013

Availability

Academic Institutions and Nonprofits only
D5 gene in PETCON vector

Plasmid

#87626

Purpose

expresses D5 allosteric Zn2+ and fluorescein binding antibody for yeast display

Depositor

Sarel Fleishman

Article

Khersonsky et al Protein Sci. 2017 Apr;26(4):807-813. doi

Insert

Designed allosteric Zn2+ and fluorescein binding antibody scFv D5

Tags

3x(G4S) Linker and cMyc tag on backbone

Expression

Yeast

Available Since

March 29, 2017

Availability

Academic Institutions and Nonprofits only

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YIplac211-msGFP2-STE13

Plasmid

#177106

Purpose

Expresses msGFP2-Ste13 in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

STE13 (STE13 Budding Yeast)

Expression

Yeast

Available Since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pT2-cryR;basPEgfp

Plasmid

#90169

Purpose

contains a basal promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

basalPEgfp

Tags

EGFP

Expression

Bacterial

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pHL661

Plasmid

#37635

Depositor

Han Lim

Article

Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110

Inserts

GFP
mCherry

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLlacO-1 and pLtetO-1

Available Since

Aug. 24, 2012

Availability

Academic Institutions and Nonprofits only
pICSL1P3_AtU626Ter26

Plasmid

#191771

Purpose

Cas9 guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

SpCas9 guide scaffold with AtU626 promoter/terminator

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCsy-crRNA-EV

Plasmid

#153944

Purpose

For cloning and expression of Type I-F P. aeruginosa (PaeCascade) crRNA in mammalian cells

Depositor

Zhou Songyang

Article

Chen et al Nat Commun. 2020 Jun 19;11(1):3136. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pHCKan-yibDp-GST

Plasmid

#134592

Purpose

Low Phosphate inducible GST

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

Glutathione S-transferase

Tags

none

Expression

Bacterial

Promoter

yibD

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pDD375

Plasmid

#91825

Purpose

Codon-optimized mKate2 donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

mKate2-C1

Use

CRISPR

Expression

Worm

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pBT100.136c

Plasmid

#122603

Purpose

clone FERNY phage by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

FERNY

Expression

Bacterial

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pPJ167(JPUB_006765)

Plasmid

#83831

Purpose

Carries NEW operon 3 final

Depositor

Harry Beller

Article

Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

Inserts

Kuste2803
Kuste2804

Promoter

op3_2804_for and op3_BsmBI_2803_for

Available Since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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SNX19-GFP

Plasmid

#197392

Purpose

Expresses SNX19 in mammalian cells

Depositor

Juan Bonifacino

Article

Saric et al Nat Commun. 2021 Jul 27;12(1):4552. doi:

Insert

SNX19 (SNX19 Human)

Tags

GFP

Expression

Mammalian

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCDF-CcAvs4

Plasmid

#188808

Purpose

Codon optimized CcAvs4 with C-terminal His tag

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

KpAvs4

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pRATIO2151

Plasmid

#141300

Purpose

Gateway cloning compatible ratiometric binary vector

Depositor

David Nelson

Article

Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

Type

Empty backbone

Tags

Firefly Luciferase or LUC2 and mScarlet-I

Expression

Plant

Promoter

UBQ10 promoter

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pDD397

Plasmid

#91822

Purpose

AID FP-slot donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

Auxin-inducible degron (AID)

Use

CRISPR

Expression

Worm

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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