Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 12,030 results for: LEA

Clear keywords
Showing: 11521 - 11540 of 12030 results

  1. pCEP4-HA-Flag-natT1R2 d835

    Plasmid
    #113959
    Purpose
    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant
    Depositor
    Erik Procko
    Article
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R2 (TAS1R2 Human)
    Tags
    FLAG and Signal/leader sequence from influenza he…
    Expression
    Mammalian
    Mutation
    residues 22-834
    Promoter
    cmv
    Available Since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113959-plasmid-map-sequence-id-215288

    Enlarge

  2. N229A Lo-MetQ

    Plasmid
    #118268
    Purpose
    N229A Mutation in Methionine Binding Protein (MetQ) used to solve crystal structure
    Depositor
    Douglas Rees
    Article
    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p
    Insert
    MetQ (N229A) (metQ E. coli)
    Tags
    10x His Tag and Enterokinase Cleavage Site
    Expression
    Bacterial
    Mutation
    Changed Asparagine (Asn) 229 to Alanine (Ala)
    Promoter
    T7 Promoter
    Available Since
    Jan. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    118268-plasmid-map-sequence-id-228534

    Enlarge

  4. pET-30a(+)-N-SH2

    Plasmid
    #111274
    Purpose
    express murine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site, in E.coli strain Rosetta 2 (DE3)
    Depositor
    Carol Post
    Article
    Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5
    Insert
    murine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site (Syk Mouse)
    Tags
    HHHHHHENLYFQG
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    July 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    111274-plasmid-map-sequence-id-215709

    Enlarge

  11. pET-Cb2

    Plasmid
    #53529
    Purpose
    Expresses an insect mitochondrial arginyl-tRNA synthetase in E.coli
    Depositor
    Gabor Igloi
    Article
    Leisinger et al Insect Biochem Mol Biol. 2013 Dec;43(12)
    Insert
    mitochondrial arginyl-tRNA synthetase
    Tags
    His Tag and Thioredoxin
    Expression
    Bacterial
    Mutation
    changed Ser4 to Ala4 to permit NcoI cloning
    Promoter
    T7
    Available Since
    July 31, 2014
    Availability
    Academic Institutions and Nonprofits only
    53529-plasmid-map-sequence-id-83364

    Enlarge

  19. pGEX6p1_GST-HRV3C-DmrB-[dltCARD]mCasp1(E102D103TEV)-8xHis (KS585)

    Plasmid
    #254943
    Purpose
    Murine caspase-1 with the E102/D103 autocleavage site replaced by a TEV site, CARD replaced by DmrB, N-terminal GST followed by a PreScission (HRV3C) site, and a C-terminal 8xHis tag.
    Depositor
    Kate Schroder
    Article
    Boucher et al J Exp Med. 2018 Mar 5;215(3):827-840. do
    Insert
    Casp1 (Casp1 Mouse)
    Tags
    8xHis and GST
    Expression
    Bacterial
    Mutation
    E102D103TEV
    Promoter
    tac
    Available Since
    June 9, 2026
    Availability
    Academic Institutions and Nonprofits only
    254943-plasmid-map-sequence-id-512353

    Enlarge

Showing: 11521 - 11540 of 12030 results