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pSin-GFP-Fg

Plasmid

#174307

Purpose

Lentiviral expression of GFP

Depositor

Bruce Beutler

Article

Yue et al Science. 2021 May 14;372(6543). pii: 372

Insert

GFP (eGFP Aequorea victoria)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-Zyxin-D (aa160-340)-EGFP

Plasmid

#187696

Purpose

Visualization of Zyxin

Depositor

Mary Beckerle

Article

Hoffman et al Mol Biol Cell. 2012 May;23(10):1846-59.

Insert

Zyxin (Zyx Mouse)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa160-340

Promoter

CMV

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pFRT-PonA-BI-Gl-WT-24xMS2-PTC-18xPP7 (WP)

Plasmid

#189792

Purpose

Express wild-type and PTC-containing beta-globin genes bi-directionally under ponasteron A inducible promoter in mammalian cells

Depositor

Robert Singer

Article

Sato et al Nat Commun. 2021 Dec 10;12(1):7203. doi:

Insert

beta-globin (HBB Human, Mouse)

Expression

Mammalian

Promoter

Ponasterone A inducible

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pRc/RSV Flag MKK3

Plasmid

#14671

Depositor

Roger Davis

Article

Derijard et al Science. 1995 Feb 3. 267(5198):682-5.

Insert

MKK3 (MAP2K3 Human)

Tags

Flag

Expression

Mammalian

Available Since

July 25, 2007

Availability

Academic Institutions and Nonprofits only
pMTH PKC alpha

Plasmid

#8409

Depositor

Frederic Mushinski

Article

Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

Insert

Mouse Protein Kinase C alpha (Prkca Mouse)

Expression

Mammalian

Available Since

March 5, 2007

Availability

Academic Institutions and Nonprofits only
pBa.GFP-myosin Vb tail 1436-1846

Plasmid

#162714

Purpose

Mammalian expression of rat WT Myosin Vb with deletion of the 1435 N-terminal residues

Depositor

Marvin Bentley

Article

Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

Insert

Myosin Vb (Myo5b Rat)

Tags

GFP

Expression

Mammalian

Mutation

WT with deletion of the 1435 N-terminal residues.

Promoter

Chicken Beta actin

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-Uricase

Plasmid

#182401

Purpose

Bacterial expression of Vesicle Nucleating peptide-uricase fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-uricase

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pBABE-neo largeTcDNA K1

Plasmid

#8581

Depositor

Bob Weinberg

Article

Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Insert

large T antigen (SV40gp6 simian virus)

Use

Retroviral

Expression

Mammalian

Mutation

E107K. Alteration in pRB consensus binding motif…

Available Since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only
H1.224.M.luc

Plasmid

#193705

Purpose

Expresses a truncated PolIII H1 promoter of 224 nucleotides (H1.224) with the TATA box mutated

Depositor

Elena Herrera-Carrillo

Article

Gao et al J Biol Chem. 2020 Nov 5. pii: S0021-9258

Insert

Truncated PolIII H1 promoter (H1.224) with the TATA box mutated

Use

Luciferase

Expression

Mammalian

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast coUGP2

Plasmid

#211531

Purpose

Expresses codon optimized UGP2

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

UGP2 (UGP2 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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T/brachyury(2A-Dre)

Plasmid

#140761

Purpose

Targeting vector to T/brachyury gene locus for induction of P2A-fused Dre, with PGK-EM7-puroR drug resistant cassette flanked by FRT(F3) sequence.

Depositor

Hideyuki Okano

Article

Serizawa et al Development. 2019 Nov 4;146(21). pii: de

Insert

Dre

Use

Mouse Targeting

Promoter

Endogenous T/brachyury

Available Since

June 16, 2020

Availability

Academic Institutions and Nonprofits only

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mRIP3 GFP RHIM mut

Plasmid

#41384

Depositor

Francis Chan

Article

Cho et al Cell. 2009 Jun 12;137(6):1112-23.

Insert

RIP3 (Ripk3 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

RHIM mutant

Promoter

CMV

Available Since

Dec. 4, 2012

Availability

Academic Institutions and Nonprofits only

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pMXs-ms-Sox18

Plasmid

#50782

Purpose

retroviral expression of mouse Sox18

Depositor

Shinya Yamanaka

Article

Nakagawa et al Nat Biotechnol. 2008 Jan;26(1):101-6. Ep

Insert

Sox18 (Sox18 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

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pMXs-Scl

Plasmid

#131601

Purpose

expression of mouse ScI in mammalian cells

Depositor

Kateri Moore, Filipe Pereira

Article

Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

Insert

Scl (Tal1 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Dec. 11, 2019

Availability

Academic Institutions and Nonprofits only

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Equalizer-H plasmid

Plasmid

#169367

Purpose

Equalizer variant plasmid with high eGFP output levels but reduced gene dosage compensation capability.

Depositor

Francois St-Pierre

Article

Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

Insert

tetR-P2A-eGFP-miR(FF3) target-miR(FF3)

Use

Synthetic Biology

Expression

Mammalian

Promoter

pCMV-tetO2

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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Equalizer-M plasmid

Plasmid

#169731

Purpose

Equalizer variant plasmid with medium eGFP output levels and medium gene dosage compensation capability.

Depositor

Francois St-Pierre

Article

Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

Insert

tetR-P2A-eGFP-miR(FF4) target-miR(FF4)

Use

Synthetic Biology

Expression

Mammalian

Promoter

pCMV-tetO2

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBMN iRFP670-OPTN(D474N)

Plasmid

#119693

Purpose

For mammalian expression of iRFP-tagged UBD mutant OPTN

Depositor

Michael Lazarou

Article

Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

Insert

OPTN (OPTN Human)

Use

Retroviral

Tags

iRFP670

Expression

Mammalian

Mutation

D474N

Available Since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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AAVS-HsProm1s1-StayGold

Plasmid

#211333

Purpose

Vector for stable mammalian gene editing with C-terminally StayGold tagged human Prom1s1 (WT)

Depositor

Luke Chao

Article

Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

Insert

Prominin 1 (PROM1 Human)

Use

AAV

Tags

StayGold

Promoter

EF1a

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pQCXIH-PSM-Rb

Plasmid

#37106

Depositor

Joseph Nevins

Article

Angus et al Oncogene. 2012 Jan 16. doi: 10.1038/onc.

Insert

Rb (RB1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

aa 379-928 only; large pocket of human Rb with aa…

Promoter

CMV

Available Since

Sept. 21, 2012

Availability

Academic Institutions and Nonprofits only
pXD70CT5.1-PxylR-CymR, Pveg(2CuO)-LacZ

Plasmid

#191628

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1 (tandem CymR binding sites)-LacZ

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

lacZ

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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