We narrowed to 75,715 results for: Rest
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Plasmid#86632PurposeExpresses eGFP-Akt2-HaloTag-PS fusion (cytosolic tandem)DepositorInsertAkt2(K14A/R25E)-Halo-PS (AKT2 )
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFBD-Akt13C(WT)
Plasmid#86576PurposeExpresses cleavable human full-length Akt1(WT)DepositorInsertAkt1 (AKT1 Human)
UseTags10xHisExpressionInsectMutation3C cleavage site inserted at pos. 130PromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
CC1
Plasmid#63713PurposeWild-type voltage sensing domain from Ciona voltage sensing phosphatase fused to super ecliptic A227D with restriction site flanking each transmembrane domainDepositorInsertCC1
UseTagsSuper ecliptic pHlorin A227DExpressionMammalianMutationPromoterCMVAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBabe_puro_DEST_MYNN
Plasmid#45260DepositorInsertMYNN (MYNN Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLEX_970_puro_DEST_V5_TLOC1(220aa)deltaN81
Plasmid#45293DepositorInsertTLOC1 (SEC62 Human)
UseLentiviralTagsV5ExpressionMammalianMutationdeleted 81 N-terminal amino acidsPromoterAvailable sinceMay 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pWzl_neo_DEST_flag_SKIL
Plasmid#45303DepositorInsertSNON (SKIL Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceMay 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLEX_970_puro_DEST_V5_TLOC1(220aa)deltaC27
Plasmid#45292DepositorInsertTLOC1 (SEC62 Human)
UseLentiviralTagsV5ExpressionMammalianMutationdeleted 27 c-terminal amino acidsPromoterAvailable sinceMay 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLEX_970_puro_DEST_V5_TLOC1(220aa)deltaN149
Plasmid#45294DepositorInsertTLOC1 (SEC62 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceMay 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
ALFA-bArr1
Plasmid#201495PurposeExpression of the beta-arrestin1 containing a N-terminal ALFA tag.DepositorInsertALFA-bArr1 (ARRB1 Human)
UseTagsALFAExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
Flag-ZDHHC9
Plasmid#231658PurposeExpresses human ZDHHC9 proteinDepositorInsertZDHHC9 (ZDHHC9 Human)
UseTags3Ă—Flag TagExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
Flag-ZDHHC17
Plasmid#231665PurposeExpresses human ZDHHC17proteinDepositorInsertZDHHC17 (ZDHHC17 Human)
UseTags3Ă—Flag TagExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
Flag-ZDHHC21
Plasmid#231669PurposeExpresses human ZDHHC21proteinDepositorInsertZDHHC21 (ZDHHC21 Human)
UseTags3Ă—Flag TagExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHR-scFv-GCN4-sfGFP-GB1-NLS-iLID-dWPRE
Plasmid#121966PurposeExpresses fusion of single chain variable fragment recognizing SunTag, fluorescent protein sfGFP, and iLID which upon light activation binds to sspB.DepositorInsertscFv-GCN4
UseLentiviralTagssuperfold GFP-GB1-NLS-iLIDExpressionMammalianMutationPromoterSFFVAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
Flag-ZDHHC8
Plasmid#231657PurposeExpresses human ZDHHC8 proteinDepositorInsertZDHHC8 (ZDHHC8 Human)
UseTags3Ă—Flag TagExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
Flag-ZDHHC7
Plasmid#231656PurposeExpresses human ZDHHC7 proteinDepositorInsertZDHHC7 (ZDHHC7 Human)
UseTags3Ă—Flag TagExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
Flag-ZDHHC3
Plasmid#231652PurposeExpresses human ZDHHC3 proteinDepositorInsertDHHC3 (ZDHHC3 Human)
UseTags3Ă—Flag TagExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti_Ef1a:PE2-P2A-GFP
Plasmid#184445PurposeSingle EF1a-driven PE2-P2A-GFP (Addgene #132776) and PaqCI restriction sites for pegRNA cloning. Lentiviral backbone and AmpR.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 and Ef1AAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTA-Mob 2.0 Tp
Plasmid#188619PurposeMutated pTA-Mob 2.0 Backbone in TraJ Promoter RegionDepositorTypeEmpty backboneUseSynthetic Biology; Conjugation helper plasmidTagsExpressionBacterial and YeastMutationPromoterAvailable sinceMay 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEM5Z(+)-EGFP
Plasmid#65206PurposeEGFP expression plasmid (also called p111) for the creation of a heteroduplex DNA repair assay plasmid with p189. *Please see notes in comments section.DepositorInsertEGFP
UseTagsExpressionBacterial and MammalianMutationRestriction enzyme recognition sites added either…PromoterCMVAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV-SC4
Plasmid#236254PurposeAAV-SC4 capsid which targets peripheral nerves in miceDepositorInsertsrep/cap AAV9 VP1 with GATPSTQ peptide insert
rep
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-fDIO-pAce-Kv2.1PR
Plasmid#195535PurposeFlp recombinase-dependent green fluorescent, positive response-polarity voltage indicator; soma-targetedDepositorInsertpAce-Kv2.1 proximal restriction sequence
UseAAVTagsExpressionMammalianMutationAce-mNeon 78K, 81D, 92N, 178F; SY linkerPromoterCAGAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
PGK-DTAG-CTERM-GFP
Plasmid#185770PurposeDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloningDepositorInsertGFP
UseLentiviralTagsV5, dTAGExpressionMutationnonePromoterPGKAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHIV-EF1a-2A-Ovalbumin(dN)-ZsGreen
Plasmid#231986PurposeEmpty backbone for expression of Ovalbumin with Bi-cistronic expression ZsGreenDepositorInsertOVA-2A-ZsGreen
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.3 HA-hTSPAN12
Plasmid#115784PurposeExpresses human TSPAN12 with N-terminal HA-tag in mammalian cells. Contains multiple silent mutations that introduce restriction sites.DepositorInsertTSPAN12 (TSPAN12 Human)
UseTagsHAExpressionMammalianMutationmultiple silent mutations introduce restriction s…PromoterCMVAvailable sinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv2 sgIRF3#5
Plasmid#127642PurposeKnock-out of human IRF3DepositorInsertIRF3 sgRNA (IRF3 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv2 Neo sgTREX1
Plasmid#127645PurposeKnock-out of human TREX1 with NeoRDepositorInsertTREX1 sgRNA (TREX1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
POLD1_pLX307
Plasmid#98358PurposeLentiviral expression of POLD1DepositorInsertPOLD1 (POLD1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterE1FaAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
PGK-DTAG-NTERM-GFP
Plasmid#185775PurposeDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloningDepositorInsertGFP
UseLentiviralTagsV5, dTAGExpressionMutationnonePromoterPGKAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
TLCV2-Scal-T2A-EGFP-Puro
Plasmid#234949PurposeDoxycycline-inducible mitochondria-localized ScaI expressing plasmidDepositorInsertScaI restriction enzyme with N-terminal COX8 mitochondrial localization signal
UseLentiviralTagsFLAGExpressionMammalianMutationPromotertight TRE promoterAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
TLCV2-ApaLI-T2A-EGFP-Puro
Plasmid#234948PurposeDoxycycline-inducible mitochondria-localized ApaLI expressing plasmidDepositorInsertApaLI restriction enzyme with N-terminal COX8 mitochondrial localization signal
UseLentiviralTagsFLAGExpressionMammalianMutationPromotertight TRE promoterAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER21-SOX4
Plasmid#51304PurposeExpression of SOX4 after doxycycline administrationDepositorInsertSRY (sex determining region Y)-box 4 (SOX4 Human)
UseLentiviralTagsn/aExpressionMutationfull length ORFPromoterAvailable sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-dCas9/pCMV-7.1
Plasmid#47948PurposeExpresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationhuman codon-optimized, D10A + H840A (catalyticall…PromoterCMVAvailable sinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
TCR012H
Plasmid#233454PurposeExpresses ncHLAp-reactive T cell receptor (TCR012H)DepositorInsertTCR012H
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-GABRA1(A322D)-IRES2-EGFP
Plasmid#170821Purposehuman GABAA receptors alpha1 subunit carrying A322D mutation and IRES2-EGFPDepositorInsertHuman GABAA receptor alpha1 subunit carrying A322D mutation (GABRA1 Human)
UseTagsIRES2-EGFPExpressionMammalianMutationchange Alanine 322 to AspartatePromoterAvailable sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro-irf3-shrna1
Plasmid#127648PurposeKnock-down of human IRF3DepositorInsertIRF3 shRNA (IRF3 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
PGK-SMASH-CTERM-GFP
Plasmid#185776PurposeDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloningDepositorInsertGFP
UseLentiviralTagsV5, SMAShExpressionMutationnonePromoterPGKAvailable sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-pAce-Kv2.1PR
Plasmid#195529PurposeGreen fluorescent, positive response-polarity voltage indicator under the control of CaMKII promoter; soma-targetedDepositorInsertpAce-Kv2.1 proximal restriction sequence
UseAAVTagsExpressionMammalianMutationAce-mNeon 78K, 81D, 92N, 178F; SY linkerPromoterCaMKIIAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-pAceR-Kv2.1PR
Plasmid#195534PurposeCre-dependent red fluorescent, positive response-polarity voltage indicator; soma-targetedDepositorInsertpAceR-Kv2.1 proximal restriction sequence
UseAAV and Cre/LoxTagsExpressionMammalianMutationVARNAM 78E, 81D, 92N; SI linkerPromoterCAGAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
PGK-IKZF3-CTERM-GFP
Plasmid#185779PurposeDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloningDepositorInsertGFP
UseLentiviralTagsV5, IKZF3 minidegronExpressionMutationnonePromoterPGKAvailable sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-Ace-mNeon2-Kv2.1PR
Plasmid#195532PurposeCre-dependent expression of a green fluorescent, negative response-polarity voltage indicator; soma-targetedDepositorInsertAce-mNeon2-Kv2.1 proximal restriction sequence
UseAAV and Cre/LoxTagsExpressionMammalianMutationAce-mNeon 81S; SY linkerPromoterCAGAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCI-neoBeta2mcherry
Plasmid#45097DepositorInsertnAChR beta2 mcherry (Chrnb2 Mouse)
UseTagshas c-myc tagExpressionMammalianMutationβ2 was cloned into pCI-neo at the restriction sit…PromoterCMVAvailable sinceJune 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
PGK-HALO-CTERM-GFP
Plasmid#185777PurposeDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloningDepositorInsertGFP
UseLentiviralTagsV5, HaloExpressionMutationnonePromoterPGKAvailable sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
SFFV-DTAG-NTERM-GFP
Plasmid#185760PurposeDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloningDepositorInsertGFP
UseLentiviralTagsV5, dTAGExpressionMutationnonePromoterSFFVAvailable sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
PGK-AID-CTERM-GFP
Plasmid#185778PurposeDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloningDepositorInsertGFP
UseLentiviralTagsV5, AIDExpressionMutationnonePromoterPGKAvailable sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
PGK-SMASH-NTERM-GFP
Plasmid#185771PurposeDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloningDepositorInsertGFP
UseLentiviralTagsV5, SMAShExpressionMutationnonePromoterPGKAvailable sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
PGK-HALO-NTERM-GFP
Plasmid#185772PurposeDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloningDepositorInsertGFP
UseLentiviralTagsV5, HaloExpressionMutationnonePromoterPGKAvailable sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFBD-Akt13C(S473D)
Plasmid#86577PurposeExpresses cleavable human full-length Akt1(S473D) phosphomimeticDepositorInsertAkt1 (AKT1 Human)
UseTags10xHisExpressionInsectMutation3C cleavage site inserted at pos. 130; S473D phos…PromoterAvailable sinceApril 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
SFFV-IKZF3-CTERM-GFP
Plasmid#185769PurposeDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloningDepositorInsertGFP
UseLentiviralTagsV5, IKZF3 minidegronExpressionMutationnonePromoterSFFVAvailable sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
PGK-IKZF3-NTERM-GFP
Plasmid#185774PurposeDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloningDepositorInsertGFP
UseLentiviralTagsV5, IKZF3 minidegronExpressionMutationnonePromoterPGKAvailable sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
SFFV-DTAG-CTERM-GFP
Plasmid#185765PurposeDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloningDepositorInsertGFP
UseLentiviralTagsV5, dTAGExpressionMutationnonePromoterSFFVAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only