We narrowed to 5,565 results for: SPI

1. pGBW-m4046347

   Plasmid

   #145546

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp9

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp9 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;StrepII

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pGAL1

   Available since

   April 28, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pGBW-m4046525

   Plasmid

   #145523

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp7

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp7 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;StrepII

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pGAL1

   Available since

   April 28, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pGBW-m4046290

   Plasmid

   #145522

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp10

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp10 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;StrepII

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pGAL1

   Available since

   April 28, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pGBW-m4046466

   Plasmid

   #145466

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp6

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp6 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;StrepII

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pTEF1

   Available since

   April 27, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pGBW-m4046218

   Plasmid

   #145498

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp2

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp2 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;StrepII

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pGAL1

   Available since

   April 27, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pTK678

   Plasmid

   #114707

   Purpose

   Express NuMA(1-1895)-RFP-Nano from Rosa 26 locus

   Depositor

   Tomomi Kiyomitsu

   Article

   Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

   Insert

   NuMA(1-1895)-RFP-Nano
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTK652

   Plasmid

   #114700

   Purpose

   Express NuMA(1-1700)-RFP-Nano from Rosa 26 locus

   Depositor

   Tomomi Kiyomitsu

   Article

   Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

   Insert

   NuMA(1-1700)-RFP-Nano
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTK642

   Plasmid

   #114706

   Purpose

   Express NuMA(1-213)-RFP-Nano from Rosa 26 locus

   Depositor

   Tomomi Kiyomitsu

   Article

   Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

   Insert

   NuMA(1-213)-RFP-Nano
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTK641

   Plasmid

   #114705

   Purpose

   Express NuMA(1-413)-RFP-Nano from Rosa 26 locus

   Depositor

   Tomomi Kiyomitsu

   Article

   Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

   Insert

   NuMA(1-413)-RFP-Nano
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTK664

    Plasmid

    #114704

    Purpose

    Express NuMA(1-505)-RFP-Nano from Rosa 26 locus

    Depositor

    Tomomi Kiyomitsu

    Article

    Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

    Insert

    NuMA(1-505)-RFP-Nano
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTK660

    Plasmid

    #114702

    Purpose

    Express NuMA(1-705)4A-RFP-Nano from Rosa 26 locus

    Depositor

    Tomomi Kiyomitsu

    Article

    Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

    Insert

    NuMA(1-705)4A-RFP-Nano
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTK631

    Plasmid

    #114701

    Purpose

    Express NuMA(1-705)-RFP-Nano from Rosa 26 locus

    Depositor

    Tomomi Kiyomitsu

    Article

    Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

    Insert

    NuMA(1-705)-RFP-Nano
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTK525

    Plasmid

    #114691

    Purpose

    sgRNA for p150's C-terminal locus

    Depositor

    Tomomi Kiyomitsu

    Article

    Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

    Insert

    p150 sgRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTK371

    Plasmid

    #114693

    Purpose

    sgRNA for DHC's N-terminal locus

    Depositor

    Tomomi Kiyomitsu

    Article

    Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

    Insert

    DHC-N sgRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AAEL017410 MIP R

    Plasmid

    #115411

    Purpose

    Expresses Ae. aegypti receptor in mammalian cells

    Depositor

    Leslie Vosshall

    Article

    Duvall et al Cell. 2019 Feb 7;176(4):687-701.e5. doi:

    Insert

    CCHamide-2 receptor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AAEL008296 CCAP R

    Plasmid

    #115402

    Purpose

    Expresses Ae. aegypti receptor in mammalian cells

    Depositor

    Leslie Vosshall

    Article

    Duvall et al Cell. 2019 Feb 7;176(4):687-701.e5. doi:

    Insert

    ovo isoform X1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AAEL017414 TK R

    Plasmid

    #115396

    Purpose

    Expresses Ae. aegypti receptor in mammalian cells

    Depositor

    Leslie Vosshall

    Article

    Duvall et al Cell. 2019 Feb 7;176(4):687-701.e5. doi:

    Insert

    phenoloxidase 2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTK645

    Plasmid

    #114711

    Purpose

    Express NuMA(1700-2115)-RFP-Nano from Rosa 26 locus

    Depositor

    Tomomi Kiyomitsu

    Article

    Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

    Insert

    NuMA(1700-2115)-RFP-Nano
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLBH531_MBP-Cas14a1 expression

    Plasmid

    #112500

    Purpose

    Expresses MBP fused to Cas14a1

    Depositor

    Jennifer Doudna

    Article

    Harrington et al Science. 2018 Oct 18. pii: science.aav42

    Insert

    Cas14a1
    

    Use

    CRISPR

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHEN2-SG4

    Plasmid

    #196071

    Purpose

    Express wild type SG4 in E.coli

    Depositor

    Shankar Balasubramanian

    Article

    Galli et al J Am Chem Soc. 2022 Dec 9. doi: 10.1021/

    Insert

    SG4
    

    Use

    Tags

    3XFLAG and 6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pTK168_mCherry-H2B

    Plasmid

    #46363

    Purpose

    visualizes chromosomes

    Depositor

    Iain Cheeseman

    Article

    Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

    Insert

    H2B (H2BC11 Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    D26G, V119I and S125D

    Promoter

    pBABE

    Available since

    Aug. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET-mGinger1

    Plasmid

    #119784

    Purpose

    Expresses mGinger1 tagged N-terminally with an MHHHHHHGSG tag

    Depositor

    Stephen Mayo

    Article

    Wannier et al Proc Natl Acad Sci U S A. 2018 Nov 27;11

    Insert

    mGinger1
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBSV2B

    Plasmid

    #118228

    Purpose

    Empty E. coli - B. burgdorferi shuttle vector, blasticidin S resistant

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET-mKelly2

    Plasmid

    #119783

    Purpose

    Expresses mKelly2 tagged N-terminally with an MHHHHHHGSG tag

    Depositor

    Stephen Mayo

    Article

    Wannier et al Proc Natl Acad Sci U S A. 2018 Nov 27;11

    Insert

    mKelly2
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET-mKelly1

    Plasmid

    #119782

    Purpose

    Expresses mKelly1 tagged N-terminally with an MHHHHHHGSG tag

    Depositor

    Stephen Mayo

    Article

    Wannier et al Proc Natl Acad Sci U S A. 2018 Nov 27;11

    Insert

    mKelly1
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCMV_psychLight2

    Plasmid

    #163910

    Purpose

    Expresses psychLight2 in mammalian cells

    Depositor

    Lin Tian

    Article

    Dong et al Cell. 2021 Apr 8. pii: S0092-8674(21)003

    Insert

    psychLight2
    

    Use

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pVC_CRISPR_Cat

    Plasmid

    #120368

    Purpose

    Reporter plasmid for full-site selection assay

    Depositor

    Jennifer Doudna

    Article

    Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

    Insert

    CRISPR_Cat
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. EKARet-cyto

    Plasmid

    #96895

    Purpose

    FLIM sensor for ERK activation in cytosol

    Depositor

    Ryohei Yasuda

    Article

    Tang et al Neuron. 2017 Mar 22;93(6):1315-1324.e3.

    Insert

    FLIM Sensor for ERK Activity in Cytosol
    

    Use

    Tags

    EGFP and sREAChet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCMV_psychLight1

    Plasmid

    #198739

    Purpose

    Expresses psychLight1 in mammalian cells

    Depositor

    Lin Tian

    Article

    Dong et al Cell. 2021 Apr 8. pii: S0092-8674(21)003

    Insert

    psychLight1
    

    Use

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. mRFP-RBD(R59A)-mRFP

    Plasmid

    #18664

    Depositor

    Karel Svoboda

    Article

    Yasuda et al Nat Neurosci. 2006 Feb . 9(2):283-91.

    Insert

    Ras binding domain of Raf
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    R59A mutation in RBD

    Promoter

    Available since

    July 2, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pHB9-EGFP (Hb9 promoter) [TJ#96]

    Plasmid

    #16275

    Depositor

    Thomas Jessell

    Article

    Wilson et al J Neurosci. 2005 Jun 15. 25(24):5710-9.

    Insert

    HB9 promoter
    

    Use

    Mouse Targeting

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Plk1 FRET sensor c-jun substrate

    Plasmid

    #45203

    Depositor

    Michael Lampson

    Article

    Liu et al J Cell Biol. 2012 Aug 20;198(4):491-9. d

    Insert

    Plk1 FRET sensor with c-jun substrate
    

    Use

    Tags

    CyPET, YPET

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    June 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLBH533_MBP-Cas14b2 expression

    Plasmid

    #112506

    Purpose

    Expresses 10xHis-MBP fused to Cas14b2 under t7 promoter

    Depositor

    Jennifer Doudna

    Article

    Harrington et al Science. 2018 Oct 18. pii: science.aav42

    Insert

    Cas14b2
    

    Use

    CRISPR

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLBH532_MBP-Cas14b1 expression

    Plasmid

    #112505

    Purpose

    Expresses 10xHis-MBP fused to Cas14b1 under t7 promoter

    Depositor

    Jennifer Doudna

    Article

    Harrington et al Science. 2018 Oct 18. pii: science.aav42

    Insert

    Cas14b1
    

    Use

    CRISPR

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. EKARet-nuc

    Plasmid

    #96896

    Purpose

    FLIM sensor for ERK activation in nucleus

    Depositor

    Ryohei Yasuda

    Article

    Tang et al Neuron. 2017 Mar 22;93(6):1315-1324.e3.

    Insert

    FLIM Sensor for ERK Activity in Nucleus
    

    Use

    Tags

    EGFP and sREAChet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pScalps hygro

    Plasmid

    #184785

    Purpose

    Empty Lentiviral vector backbone

    Depositor

    Ajit Chande

    Article

    Mishra et al Front Cell Infect Microbiol. 2021 Apr 23

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Sept. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLenti-CamkII-MacQ-mCitrine

    Plasmid

    #48762

    Purpose

    sensor

    Depositor

    Mark Schnitzer

    Article

    Gong et al Nat Commun. 2014 Apr 22;5:3674. doi: 10.

    Insert

    macQ-mCitrine
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    mac-D139Q

    Promoter

    CamKIIa

    Available since

    Jan. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pTK50_GFP-NuMA-N

    Plasmid

    #46348

    Depositor

    Iain Cheeseman

    Article

    Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

    Insert

    NuMA N-terminal fragment (NUMA1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    N-terminal fragment from 1-213

    Promoter

    pBABE

    Available since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLenti-CamkII-MacQ-mOrange2

    Plasmid

    #48761

    Purpose

    sensor

    Depositor

    Mark Schnitzer

    Article

    Gong et al Nat Commun. 2014 Apr 22;5:3674. doi: 10.

    Insert

    macQ-mOrange
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    mac-D139Q

    Promoter

    CamKIIa

    Available since

    Jan. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pTK51_GFP-NuMA-C

    Plasmid

    #46349

    Depositor

    Iain Cheeseman

    Article

    Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

    Insert

    NuMA C-terminal fragment (NUMA1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    C-terminal fragment from 1700-2115

    Promoter

    pBABE

    Available since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. AKARet-cyto

    Plasmid

    #96897

    Purpose

    FLIM sensor for PKA activation in cytosol

    Depositor

    Ryohei Yasuda

    Article

    Tang et al Neuron. 2017 Mar 22;93(6):1315-1324.e3.

    Insert

    FLIM Sensor for PKA Activity in Cytosol
    

    Use

    Tags

    EGFP and sREAChet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. N-term Hec1 Plk1 FRET sensor c-jun substrate

    Plasmid

    #45204

    Depositor

    Michael Lampson

    Article

    Liu et al J Cell Biol. 2012 Aug 20;198(4):491-9. d

    Insert

    Hec1-targeted Plk1 FRET sensor
    

    Use

    Tags

    CyPET, YPET

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pGBW-m4046233

    Plasmid

    #149032

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp3

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp3 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

44. pGBW-m4046664

    Plasmid

    #149224

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 E (envelope)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 E (envelope) (E Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

45. pGBW-m4252984

    Plasmid

    #153898

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 SARS-CoV-2 E (envelope)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

46. pGBW-m4046479

    Plasmid

    #145495

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp4

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp4 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

47. pGBW-m4046323

    Plasmid

    #149100

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp3

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp3 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;MBP

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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48. pGBW-m4252975

    Plasmid

    #153873

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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49. pJMH147

    Plasmid

    #186647

    Purpose

    aadA.co-TaadA-J23107-4ABH.co.rm1-PpsaA-nhoA.co.rm1 between homology arms for pilA

    Depositor

    Adam Arkin

    Article

    Jacob M. et al bioRxiv

    Insert

    aadA.co-TaadA-J23107-4ABH.co.rm1-PpsaA-nhoA.co.rm1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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50. pGBW-m4134165

    Plasmid

    #152085

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;3xFlag

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available since

    May 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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