We narrowed to 1,268 results for: abo.1
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Plasmid#204236PurposeBacterial expression of Arabidopsis mitochondrial malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).InsertMDHM1_ARATH (mMDH1 Mustard Weed)
UseTags6X HisExpressionBacterialMutationDoes done include the first 52 residues that are …PromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
DBDmut-CA-RIT-NFAT1
Plasmid#63669PurposeConstitutively active NFAT1 and also unable to interact with AP-1 with four point mutations in the DNA binding loop that abolish DNA bindingDepositorInsertNFAT1 (Nfatc2 Mouse)
UseRetroviralTags3x HAExpressionMammalianMutationCA: PASSGSSASF mutated to PAAAGAAAAF, SPRTSPIMSP…PromoterAvailable sinceJuly 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BACH1t_RFP
Plasmid#199214PurposeBACH1 C-terminus truncated isoform - BACH1t expression plasmid with a RFP reporter on the backbone.DepositorInsertsUseTagsExpressionMammalianMutationPromoterCMV and PGKAvailable sinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP Cav2.2 HA EI,II,III,IVA (E314A, E664A, E1370A, E1658A) pcDNA3
Plasmid#206093PurposeCav2.2 calcium channel with a N-terminal GFP tag, exofacial double HA tag in domain II and mutations in the P loop of Domains I, II, III and IV which abolish trafficking to the cell surfaceDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsGFPExpressionMammalianMutationE314A, E664A, E1370A, E1658APromoterCMVAvailable sinceNov. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 HA EI,II,III,IVA (E314A, E664A, E1370A, E1658A) pCAGGS
Plasmid#206098Purposeexpression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and mutations in the P loop of Domains I, II, III and IV which abolish trafficking to the cell surfaceDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationE314A, E664A, E1370A, E1658APromoterCMV/B-actinAvailable sinceNov. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmiRFP-H2B-C1
Plasmid#108411PurposeHistone 2B fused to monomeric near-infrared fluorescent protein miRFPDepositorInsertmiRFP-H2B (H2BC21 Human)
UseTagsmiRFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR8/GW_TOPO_Cdk13_Untagged
Plasmid#127342PurposeUntagged mouse Cdk13 transgene (NP_001074527.1 isoform) cloned into Gateway entry vectorDepositorInsertCyclin Dependent Kinase 13 (Cdk13 Mouse)
UseEntry vector with transgene for gateway cloningTagsExpressionMutationBase pairs 1-1554 of transgene are codon optimize…PromoterNoneAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
G protein alpha-s-mCherry
Plasmid#66968PurposeG protein alpha-s internally tagged with mCherry and EE epitopeDepositorInsertG-alpha-s-EE-mCherry (Gnas Rat, Discosoma sp.)
UseTagsinternal EE epitope (residues 189-194 in alpha- s…ExpressionMammalianMutationBamHI sites in the alpha-s cDNA were removed and …PromoterCMVAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
G protein alpha-s-CFP
Plasmid#55793PurposeG protein alpha-s internally tagged with ECFP and EE epitopeDepositorInsertG-alpha-s-EE-ECFP (Gnas Rat)
UseTagsECFP flanked by SGGGS on each side was inserted i…ExpressionMammalianMutationHis was substituted for Asn164 in ECFP (Clontech)…PromoterCMVAvailable sinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
G protein alpha-s-mCerulean
Plasmid#66084PurposeG protein alpha-s internally tagged with mCerulean and EE epitopeDepositorInsertG-alpha-s-EE-mCerulean (Gnas Rat, Aequorea victoria)
UseTagsinternal EE epitope (residues 189-194 in alpha-s …ExpressionMammalianMutationBamHI sites in the alpha-s cDNA were removed and …PromoterCMVAvailable sinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCR8/GW_TOPO_Cdk13_Nterm_FlagHa
Plasmid#127343PurposeN-terminal Flag- HA- tandem epitope tagged mouse Cdk13 transgene (NP_001074527.1 isoform) cloned into Gateway entry vectorDepositorInsertCyclin Dependent Kinase 13 (Cdk13 Mouse)
UseEntry vector with transgene for gateway cloningTagsFlag- HA- Tandem EpitopesExpressionMutationBase pairs 1-1554 of transgene are codon optimize…PromoterNoneAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB-Neo-TetO-Cdk13_Untagged
Plasmid#127344PurposeUntagged mouse Cdk13 transgene (NP_001074527.1 isoform) cloned into a dox-inducible piggybac destination vectorDepositorInsertCyclin Dependent Kinase 13 (Cdk13 Mouse)
UsePiggybac transposase vectorTagsNoneExpressionMammalianMutationBase pairs 1-1554 of transgene are codon optimize…PromoterTetO PromoterAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDUET-aaCPRct-A13AMO
Plasmid#20116DepositorInsertp450 reductase and Amorphadiene oxidase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBbA5k-EPL12
Plasmid#45404DepositorInsertPP_3456
UseSynthetic BiologyTagsHisExpressionMutationPromoterLacUV5Available sinceJan. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBbA5k-EPL66
Plasmid#45425DepositorInsertPSPPH_0329
UseSynthetic BiologyTagsHisExpressionMutationPromoterLacUV5Available sinceJune 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAEF6
Plasmid#136303PurposepAEF5 plasmid with two gRNA insertedDepositorInsertdouble cassette coding for gRNA
UseCRISPR; Template for pcr to insert double grna co…TagsExpressionMutationPromoterAvailable sinceFeb. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
GRM1
Plasmid#25353PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertGRM1-C254S:L28-G518 (GRM1 Human)
UseTagsHisExpressionInsectMutationC254S. Contains amino acids L28-G518.PromoterAvailable sinceJuly 29, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLEX_307-SOD1WT
Plasmid#83444PurposeMammalian expression of SOD1WTDepositorInsertSOD1 (SOD1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1αAvailable sinceNov. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLEX_307-SOD1E133insTT
Plasmid#83446PurposeMammalian expression of SOD1E133insTT.DepositorInsertSOD1 (SOD1 Human)
UseLentiviralTagsExpressionMammalianMutationE133insTT (after GA)PromoterEF-1αAvailable sinceNov. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHIS-MDH1-HIS
Plasmid#184558PurposeBacterial expression of human MDH1 with HIS TagDepositorInsertMalate dehydrogenase 1 (MDH1 Human)
UseTags6xHISExpressionBacterialMutationPromoterAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only