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pHelper_O

Plasmid

#173783

Purpose

The 186 helper plasmid necessary for genomic integration of the pIT5_KO plasmid.

Depositor

Keith Shearwin

Article

Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

Insert

186 Integrase

Use

Synthetic Biology

Expression

Bacterial

Mutation

E59G - please see depositor comments

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pHelper_W

Plasmid

#173784

Purpose

The Wphi helper plasmid necessary for genomic integration of the pIT5_KW plasmid.

Depositor

Keith Shearwin

Article

Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

Insert

Wphi Integrase

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pHelper_D

Plasmid

#173781

Purpose

The D145 helper plasmid necessary for genomic integration of the pIT5_KD plasmid.

Depositor

Keith Shearwin

Article

Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

Insert

D145 Integrase

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-Aa-artsg13 (BsaI)

Plasmid

#188279

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Cell Discov. 2019 Apr 23;5:23. doi: 10.1

Insert

Aa-artsg13

Use

CRISPR

Expression

Mammalian

Mutation

none

Promoter

U6

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pNH053

Plasmid

#42159

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

mCherry-RT-mCherry

Available Since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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pNH041

Plasmid

#42153

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

YFP

Available Since

Feb. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pNH042

Plasmid

#42154

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

mCherry

Available Since

Feb. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pNH040

Plasmid

#42152

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

GFP

Available Since

Feb. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pNH050

Plasmid

#42156

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

CFP-RT-CFP

Available Since

Feb. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pNH043

Plasmid

#42155

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

mTFP1

Available Since

Feb. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pBTK1058

Plasmid

#209548

Purpose

BTK Type 1 homology arm for Golden Gate assembly

Depositor

Jeffrey Barrick

Article

Lariviere et al bioRxiv [Preprint]. 2023 Sep 20:2023.09.

Insert

Upstream homology arm for recombination into the Snodgrassella alvi wkB2 genome to replace clpA (SALWKB2_RS04465)

Use

Synthetic Biology

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pTYC1_pPromALB_enh5898_FireflyLuc

Plasmid

#220285

Purpose

Firefly luciferase with mouse liver DNase hypersensitive site # 5898 cloned into KpnI/XhoI-digested pPromALB_FireflyLuc

Depositor

David Waxman

Article

Chang et al BMC Genomics. 2024 Dec 24;25(1):1240. do

Insert

mouse liver DNase hypersensitive site # 5898

Use

Luciferase

Promoter

minimal albumin promoter

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pTYC2_pPromALB_enh29774_FireflyLuc

Plasmid

#220286

Purpose

Firefly luciferase with mouse liver DNase hypersensitive site # 29774 cloned into KpnI/XhoI-digested pPromALB_FireflyLuc

Depositor

David Waxman

Article

Chang et al BMC Genomics. 2024 Dec 24;25(1):1240. do

Insert

mouse liver DNase hypersensitive site # 29774

Use

Luciferase

Promoter

minimal albumin promoter

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pTYC3_pPromALB_enh50363_FireflyLuc

Plasmid

#220287

Purpose

Firefly luciferase with mouse liver DNase hypersensitive site # 50363 cloned into KpnI/XhoI-digested pPromALB_FireflyLuc

Depositor

David Waxman

Article

Chang et al BMC Genomics. 2024 Dec 24;25(1):1240. do

Insert

mouse liver DNase hypersensitive site # 50363

Use

Luciferase

Promoter

minimal albumin promoter

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pTYC4_pPromALB_enh50358_FireflyLuc

Plasmid

#220288

Purpose

Firefly luciferase with mouse liver DNase hypersensitive site # 50358 cloned into KpnI/XhoI-digested pPromALB_FireflyLuc

Depositor

David Waxman

Article

Chang et al BMC Genomics. 2024 Dec 24;25(1):1240. do

Insert

mouse liver DNase hypersensitive site # 50358

Use

Luciferase

Promoter

minimal albumin promoter

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pBSsgRNA

Plasmid

#224867

Purpose

Yeast genome editing, Insertion of 20 bp oligo into sgRNA gene

Depositor

Katsunori Suzuki

Article

Kiyokawa et al Appl Microbiol Biotechnol. 2024 Jul 12;1

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pBTK1059

Plasmid

#209549

Purpose

BTK Type 5 homology arm for Golden Gate assembly

Depositor

Jeffrey Barrick

Article

Lariviere et al bioRxiv [Preprint]. 2023 Sep 20:2023.09.

Insert

Downstream homology arm for recombination into the Snodgrassella alvi wkB2 genome to replace clpA (SALWKB2_RS04465)

Use

Synthetic Biology

Available Since

Feb. 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pUC19-U6-Bs3-sgRNA (BsaI)

Plasmid

#188280

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Cell Discov. 2019 Apr 23;5:23. doi: 10.1

Insert

Bs3-sgRNA

Use

CRISPR

Expression

Mammalian

Mutation

none

Promoter

U6

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-TcCas12b-T2A-EGFP

Plasmid

#188277

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Cell Discov. 2019 Apr 23;5:23. doi: 10.1

Insert

TcCas12b

Use

CRISPR

Expression

Mammalian

Mutation

none

Promoter

CAG

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pNH052

Plasmid

#42158

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

YFP-RT-YFP

Available Since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge