We narrowed to 43,776 results for: tra

Showing: 11601 - 11620 of 43776 results

Results per page:

Sort results by:

pcDNA3.1(+)/AUG-nLuc-3XFLAG-CL1/PEST

Plasmid

#127315

Purpose

Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from AUG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and CL1/PEST

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
KAT14-pDEST10

Plasmid

#118382

Purpose

HIS-tagged insect cell expression vector with KAT14.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Insert

KAT14 (KAT14 Human)

Tags

HIS

Expression

Insect

Promoter

Polyhedrin

Available Since

May 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAPM-D4 miR30-DNMT3a ts1

Plasmid

#115865

Purpose

DNMT3a knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

DNMT3a shRNA (DNMT3A Human)

Use

Lentiviral

Promoter

SFFV

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-sapphireCKAR(T/A)

Plasmid

#118469

Purpose

Negative-control mutant for sapphireCKAR biosensor.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

sapphireCKAR(T/A)

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Contains Thr-to-Ala mutation in substrate sequenc…

Promoter

CMV

Available Since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-Tag 3A/WT myc-M1

Plasmid

#87719

Purpose

Expresses WT myc-M1 spastin isoform in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

Insert

SPAST (SPAST Human)

Tags

Myc

Expression

Mammalian

Mutation

M1 isoform

Promoter

CMV

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
sgTP53(R273)

Plasmid

#85561

Purpose

Expresses sgRNA targeting human TP53 around codon R273

Depositor

Luca Grumolato

Article

Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

Insert

tumor protein p53 (TP53 Human)

Expression

Mammalian

Available Since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only
pILGFPB5A

Plasmid

#83556

Purpose

Used to evaluate the expression output of GAL1 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

GAL1 promoter-yEGFP

Expression

Yeast

Promoter

GAL1

Available Since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMTB2-cytoBirA-2A-mCherry_Ras

Plasmid

#80066

Purpose

Beta-actin2 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

Depositor

Tatjana Sauka-Spengler

Article

Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

Insert

Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein

Use

Zebrafish biotagging

Tags

3x HA

Expression

Bacterial

Promoter

Beta-actin2

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL4.10-VEGFprom(-950 to -700)

Plasmid

#66133

Purpose

Luciferase-based reporter for VEGF promoter region (-950 to -700 bp)

Depositor

David Mu

Article

Wood et al Sci Rep. 2016 Feb 25;6:19857. doi: 10.10

Insert

Vascular Endothelial Growth Factor-A promoter region (VEGFA Human)

Use

Luciferase

Promoter

VEGF -950 to -700

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Str-KDEL_ManII-SBP-tagBFP

Plasmid

#65254

Purpose

synchronize trafficking of ManII from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Streptavidin-KDEL and truncated Mannosidase II

Tags

Streptavidin Binding Protein (SBP) and tagBFP

Expression

Mammalian

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available Since

June 3, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
Caspase-3 (C163A)-pcw107-V5

Plasmid

#64633

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

CASP3 (transcript variant beta) (CASP3 Human)

Use

Lentiviral

Tags

V5

Mutation

C163A

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
pCol1a1-frt (FVB)

Plasmid

#63575

Purpose

Targeting vector for the Col1a1 locus that contains a Neo resistance cassette flanked by FRT sites, followed by an ATG-less Hygromycine resistance gene. The homology arms match the sequence of FVB.

Depositor

Ivo Huijbers

Article

Huijbers et al Nat Protoc. 2015 Nov;10(11):1755-85. doi

Insert

Col1A-frt-hygro-pA

Use

Mouse Targeting; Frt/flpe

Expression

Bacterial and Mammalian

Promoter

None

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pHJ17

Plasmid

#48356

Purpose

Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Hygromycin/Gancyclovir selectable

Depositor

George Cross

Article

Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

Insert

Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-HYG-Ty1-TK-loxP-3' ALD UTR

Use

Cre/Lox; Knockout in t. brucei

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA HA FAK Y576/577F

Plasmid

#50505

Depositor

Kenneth Yamada

Article

Tamura et al J Biol Chem. 1999 Jul 16;274(29):20693-7

Insert

protein tyrosine kinase, mutated

Tags

HA

Expression

Mammalian

Mutation

Y576/577F

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pGL3-24p3/LCN2-282-LUC

Plasmid

#46865

Purpose

murine 24p3 minimal promoter as a luciferase reporter

Depositor

Sarah Gaffen

Article

Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

24p3/lipocalin 2 proximal promoter

Use

Luciferase

Promoter

24p3 minimal promoter

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only
pUC19-URA3-3'SEC13-GFP

Plasmid

#25445

Depositor

Benjamin Glick

Article

Rossanese et al J Cell Biol. 1999 Apr 5. 145(1):69-81.

Insert

Sec13-Green Fluorescent Protein

Expression

Yeast

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
SFG.CNa22_opt.IRES.eGFP

Plasmid

#22491

Depositor

Martin Pule

Article

Brewin et al Blood. 2009 Nov 26;114(23):4792-803. doi

Insert

Codon optimised Calcineurin A mutant 22 (PPP3CA Human)

Use

Retroviral

Mutation

V314R; Y341F

Available Since

Dec. 16, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL2 enhancer- F1 LUC

Plasmid

#22426

Depositor

William Sessa

Article

Zhang et al J Biol Chem. 1995 Jun 23. 270(25):15320-

Insert

eNOS promotor F1 (NOS3 Human)

Use

Luciferase

Tags

Luciferase reporter gene

Expression

Mammalian

Available Since

Dec. 8, 2009

Availability

Academic Institutions and Nonprofits only
pFlag-CMV2-Brd4 R1337P

Plasmid

#21939

Depositor

Eric Verdin

Article

Bisgrove et al Proc Natl Acad Sci U S A. 2007 Aug 21. 1

Insert

BRD4 (BRD4 Human)

Tags

Flag

Expression

Mammalian

Mutation

aa 1209-1362 only, R1337P

Available Since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only
pSP64T tBMPR (DM#135)

Plasmid

#15067

Depositor

Douglas Melton

Article

Graff et al Cell. 1994 Oct 7. 79(1):169-79.

Insert

BMP receptor (bmpr1a Frog)

Expression

Bacterial

Mutation

Truncated dominant negative form

Available Since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only