164,826 results

1. pAAV.CAG.GCaMP6s.WPRE.SV40

   Plasmid

   #100844

   Purpose

   AAV expression of ultrasensitive protein calcium sensor from CAG promoter

   Depositor

   GENIE Project, Douglas Kim

   Article

   Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

   Has Service

   AAV1, AAV5, and AAV9

   Insert

   GCaMP6s
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   GCaMP3-K78H T302L R303P D380Y T381R S383T R392G

   Promoter

   CAG

   Available Since

   Feb. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRK5-myc-Miro1

   Plasmid

   #47888

   Purpose

   Expresses myc tagged Miro1

   Depositor

   Pontus Aspenström

   Article

   Fransson et al J Biol Chem. 2003 Feb 21;278(8):6495-502

   Insert

   Miro1 (RHOT1 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. mApple-Rab5a-7

   Plasmid

   #54944

   Purpose

   Localization: Ras GTPase, Excitation: 568, Emission: 592

   Depositor

   Michael Davidson

   Insert

   Rab5a
   

   Use

   Tags

   mApple

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pLXSN18E6E7

   Plasmid

   #53459

   Purpose

   The plasmid, pLXSN18E6E7, is used to produce amphotropic recombinant retrovirus providing efficient gene transfers and expression of the HPV18 E6 and E7 genes.

   Depositor

   Denise Galloway

   Article

   Halbert et al J Virol. 1991 Jan;65(1):473-8.

   Insert

   HPV18E6E7
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQLink-GST-10xCTD

   Plasmid

   #138472

   Purpose

   Expresses GST-tagged 10xCTD heptapeptide repeat in bacteria

   Depositor

   Britt Glaunsinger

   Article

   Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

   Insert

   Rpb1 (POLR2A Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   10x CTD repeat

   Promoter

   T5

   Available Since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKrox24(2xD-E_inD)Luc

   Plasmid

   #200109

   Purpose

   Luciferase reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   modified EGR1 promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   highly modified sequence

   Promoter

   2 copies of D-E element in front of EGR1 promoter…

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. PX386 Lactobacillus farciminis Cas9

   Plasmid

   #68323

   Purpose

   Lactobacillus farciminis Cas9

   Depositor

   Feng Zhang

   Insert

   LfCas9
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pVC-FUS-R521C

   Plasmid

   #175067

   Purpose

   expresses mVenus and mCherry tagged to mutant of the FUS (R521C) protein in mammalian cells

   Depositor

   Arnold Boersma

   Article

   Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018

   Insert

   FUS glycine rich protein (FUS Human)
   

   Use

   Tags

   HA and mVenus + mCherry

   Expression

   Mammalian

   Mutation

   changed Arginin 521 to Cystein; mVenus: C-Termina…

   Promoter

   CMV Promoter

   Available Since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pZW1-snoVector

   Plasmid

   #73174

   Purpose

   Nuclear expression of RNAs

   Depositor

   Ling-Ling Chen

   Article

   Yin et al Nucleic Acids Res. 2015 Jan;43(1):e5. do

   Inserts

   SNORD116-13 (SNORD116-13 Human)
   SNORD116-14 (SNORD116-14 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available Since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTALYM16-Telo1

    Plasmid

    #47882

    Purpose

    TALE-mRuby2 against telomere sequence

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Miyanari et al Nat Struct Mol Biol. 2013 Oct 6. doi: 10

    Insert

    TALE-mRuby2
    

    Use

    TALEN

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Oct. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5/FRT/TO-GAlphasL-RLuc8

    Plasmid

    #140981

    Purpose

    Encodes a G alpha subunit (GNAS1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlphasL-RLuc8 (GNAS Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAT112TAG-SepT

    Plasmid

    #34624

    Depositor

    Dieter Söll, Jesse Rinehart

    Article

    Park et al Science. 2011 Aug 26;333(6046):1151-4.

    Insert

    tRNA^Sep
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lpp

    Available Since

    Feb. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEGFP VAMP7 (1-120)

    Plasmid

    #42317

    Depositor

    Thierry Galli

    Article

    Martinez-Arca et al J Cell Biol. 2000 May 15;149(4):889-900.

    Insert

    VAMP7 Longin domain (1-120) (VAMP7 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Δ120-220

    Promoter

    CMV

    Available Since

    March 1, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pInt

    Plasmid

    #18941

    Depositor

    Michele Calos

    Article

    Groth et al Proc Natl Acad Sci U S A. 2000 May 23. 9

    Insert

    phiC31 integrase (int Streptomyces phage phiC31)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    under lacZ promoter

    Promoter

    Available Since

    Aug. 13, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDR-GW AT1.03YEMK

    Plasmid

    #28004

    Depositor

    Wolf Frommer

    Article

    Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

    Insert

    AT1.03YEMK
    

    Use

    Tags

    mVenus and mseCFP

    Expression

    Yeast

    Mutation

    M60Y, G131E, K132M, 133K

    Promoter

    Available Since

    Sept. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-STAT3mts

    Plasmid

    #195577

    Purpose

    Expresses STAT3 with mitochondrial targeting sequence

    Depositor

    Kevin Park

    Article

    Luo et al Cell Rep. 2016 Apr 12;15(2):398-410. doi

    Insert

    Signal transducer and activator of transcription 3 (Stat3 Mouse)
    

    Use

    AAV

    Tags

    Cox8 presequence

    Expression

    Mammalian

    Mutation

    Promoter

    CMV enhancer and promoter

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pS648∙CsR

    Plasmid

    #197860

    Purpose

    Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria

    Depositor

    Susanne Häußler

    Article

    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

    Insert

    gRNA scaffold - Gm resistance cassette - incP oriT
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    PEM7

    Available Since

    Oct. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-NlaCascade-NLS-6xHis

    Plasmid

    #180213

    Purpose

    Expressing Neisseria lactamica Type I-C Cascade subunits (Cas5-Cas8-Cas11-Cas7) with NLS and His tag on the C terminus of Cas7 for protein purification in E.Coli

    Depositor

    Yan Zhang

    Article

    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

    Insert

    NlaCas5-Cas8-Cas7
    

    Use

    Tags

    6XHis and NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. PEA1-GFP

    Plasmid

    #171993

    Purpose

    Delivers all prime editing nickase components in a single, GFP selectable plasmid

    Depositor

    Paul Thomas

    Article

    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

    Insert

    CbH-Cas9(H840A)-RT-T2A-GFP, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV for Cas9, U6 for gRNAs

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. PCS4 3XFLAG-PPARgamma2

    Plasmid

    #78770

    Purpose

    To overexpress PPARgamma2 in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Kim et al Cell Death Differ. 2014 Apr;21(4):594-60

    Insert

    PPAR_2 (Ppargc1a Mouse)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available Since

    June 10, 2016

    Availability

    Academic Institutions and Nonprofits only