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hME-pOX

Plasmid

#178181

Purpose

Produce cRNA that expresses a human MCU-EMRE fusion protein in Xenopus oocytes

Depositor

Ming-Feng Tsai

Article

Rodriguez et al STAR Protoc. 2021 Nov 23;2(4):100979. do

Insert

human MCU-EMRE fusion protein

Tags

1D4

Expression

Bacterial

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pALPS_3'LTR GFP@gag start SFFV-

Plasmid

#101337

Purpose

Repaired U3 allows LTR-based transcription by the provirus with GFP as a marker for expression. WPRE was deleted.

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

GFP

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pH6HTC_YTHDF1-Halo

Plasmid

#175301

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

YTHDF1 (YTHDF1 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCFJ1117 - [4-1] - Promoter - Psmu-1(no_atg)

Plasmid

#159886

Purpose

Three-fragment Gateway compatible promoter with PATCs for germline expression from arrays

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[4-1] - Promoter - Psmu-1(no_atg)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pMDJ80 - pENTR[4-1] - Promoter - Psmu-2 (no_start)

Plasmid

#159887

Purpose

Three-fragment Gateway compatible promoter with PATCs for germline expression from arrays

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[4-1] - Promoter - Psmu-2 (no_start)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-MKRN1 WT

Plasmid

#78766

Purpose

To detect localization of MKRN1 in live mammalian cells

Depositor

Jaewhan Song

Article

Ko et al J Natl Cancer Inst. 2012 Nov 7;104(21):1

Insert

MKRN1 (MKRN1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only
Lenti GFP-AMOT p130 Y242/287A

Plasmid

#32829

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2011 Jan 1;25(1):51-63.

Insert

AMOT p130 (AMOT Human)

Use

Lentiviral

Tags

GFP-AMOT

Expression

Mammalian

Mutation

Y242/287A

Available Since

Feb. 22, 2012

Availability

Academic Institutions and Nonprofits only
pKD282

Plasmid

#125072

Purpose

Expression of SO_4427 under Ptac

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

SO_4427 (SO_4427 )
lacI (lacI )

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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pFL-V5-GW

Plasmid

#78857

Purpose

DULIP Prey Destination Vector (Gateway, N-terminal)

Depositor

Erich Wanker

Article

Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

Type

Empty backbone

Use

Gateway destination vector

Tags

Firefly luciferase, V5-tag

Expression

Mammalian

Promoter

CMV

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pPA-RL-GW

Plasmid

#78855

Purpose

DULIP Bait Destination Vector (Gateway, N-terminal)

Depositor

Erich Wanker

Article

Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

Type

Empty backbone

Use

Gateway destination vector

Tags

Protein A-Renilla luciferase

Expression

Mammalian

Promoter

CMV

Available Since

July 7, 2016

Availability

Academic Institutions and Nonprofits only

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pEE390

Plasmid

#149280

Purpose

T-DNA encoding TRV2 with gRNA targeting NbPDS3

Depositor

Daniel Voytas

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

p35S:TRV2_NbPDS3sgRNA:tNos

Expression

Plant

Promoter

35S

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pUCSh3-mRFP

Plasmid

#170983

Purpose

Encodes sgRNA scaffold with mRFP in place of target and Tetracycline resistance transposon. New targets can be cloned replacing mRFP using around the horn cloning.

Depositor

Christopher Reisch

Article

Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

Insert

mRFP

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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mCerulean3-SiT-N-15

Plasmid

#55445

Purpose

Localization: Golgi TGN, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

SiT (ST6GAL1 Human)

Tags

mCerulean3

Expression

Mammalian

Mutation

aa 1-45 of SiT

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only
XE7 Xwnt-8

Plasmid

#16869

Depositor

Randall Moon

Article

Christian et al Development. 1991 Apr. 111(4):1045-55.

Insert

Xwnt-8 RNA (wnt8a.L Frog)

Use

Xenopus expression

Available Since

March 13, 2008

Availability

Academic Institutions and Nonprofits only
pSicoR human DGCR8-3

Plasmid

#14771

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

Insert

DGCR8 shRNA (DGCR8 Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

April 5, 2007

Availability

Academic Institutions and Nonprofits only
pSBbi-TIR1-tTA/Neo

Plasmid

#171684

Purpose

constitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (G418)

Depositor

Randy Poon

Article

Yeung et al Mol Biol Cell. 2021 May 12:mbcE21020051.

Insert

Neomycin

Expression

Mammalian

Promoter

RPBSA promoter

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pAct:dCas9-scFv

Plasmid

#78900

Purpose

Expresses scFv-VP64 under actin promoter for SunTag CRISPRa in Drosophila cells

Depositor

Norbert Perrimon

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

scFV-VP64

Use

CRISPR

Tags

GFP

Expression

Insect

Promoter

pActin (Drosophila)

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pSuper mouse Dicer2

Plasmid

#14777

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

Insert

Dicer shRNA (Dicer1 Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
pSR26-2

Plasmid

#104335

Purpose

Expresses ompR from a TetR reuglated promoter under inducible ATC control.

Depositor

Jeffrey Tabor

Article

Landry et al Nat Commun. 2018 Apr 12;9(1):1433. doi:

Insert

OmpR (ompR Escherichia coli str. K-12 substr. MG1655)

Expression

Bacterial

Promoter

PLTetO1

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCFJ1359 - Intron GG2 - smu-1

Plasmid

#159805

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron GG2 - smu-1

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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