We narrowed to 2,304 results for: tub

1. pEB1N-LZ-LOV2(wt)

   Plasmid

   #107614

   Purpose

   N-terminal half of π-EB1 with wild-type LOV2, no fluorescent tag

   Depositor

   Torsten Wittmann

   Article

   van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

   Insert

   MAPRE1 (MAPRE1 Human)
   

   Use

   Tags

   A. sativa phototropin 1 LOV2 domain and GCN4 leuc…

   Expression

   Mammalian

   Mutation

   EB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…

   Promoter

   CMV

   Available since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. EGFP-HsBIN1_tv8

   Plasmid

   #176012

   Purpose

   Mammalian expression of Human EGFP-Bin1 (transcriptional variant 8, muscle specific). Parton lab clone HBH

   Depositor

   Rob Parton

   Article

   Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

   Insert

   Bin1
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. DN2-Gli3 in AINGpCAGGS

   Plasmid

   #121969

   Purpose

   strong Gli3 transcriptional activator

   Depositor

   James Briscoe

   Article

   Stamataki et al Genes Dev. 2005 Mar 1;19(5):626-41. doi:

   Insert

   DN2Gli3 (GLI3 Human)
   

   Use

   Chick electroporation

   Tags

   5xmyc

   Expression

   Mammalian

   Mutation

   N terminus deletion (nt1-1401)

   Promoter

   Available since

   April 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET22b-mEB2-eGFP-6xHis

   Plasmid

   #130982

   Purpose

   Bacterial expression of mouse EB2-GFP-6xHis for protein purification.

   Depositor

   Anne Straube

   Article

   Roth et al J Cell Sci. 2018 Oct 31;132(4). pii: jcs

   Insert

   EB2-GFP-6xHis (Mapre2 Mouse)
   

   Use

   Tags

   eGFP-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. Str-STIM1-NN_puromycin

   Plasmid

   #65310

   Purpose

   Luminal ER hook only (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   STIM1 mutated for binding motif to microtubule (STIM1 Human)
   

   Use

   Tags

   streptavidin

   Expression

   Mammalian

   Mutation

   2 amino acids of STIM1 (IP from SxIP motif) were …

   Promoter

   CMV

   Available since

   June 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5/FRT/TO GABARAP(G116A)-mTagBFP2

   Plasmid

   #212112

   Purpose

   Expression of C-terminally tagged GABARAP(G116A) with mTagBFP2

   Depositor

   Dieter Willbold

   Article

   Uffing et al Autophagy Rep. 2024 May 16;3(1):2348899.

   Insert

   GABARAP(G116A)-mTagBFP2 (GABARAP Human, Entacmaea quadricolor)
   

   Use

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   G116A (lipidation deficiency) of GABARAP

   Promoter

   CMV

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pXPE

   Plasmid

   #15284

   Depositor

   Ron Vale

   Article

   Navone et al J Cell Biol. 1992 Jun . 117(6):1263-75.

   Insert

   ubiquitous kinesin heavy chain (KIF5B Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   The insert is missing the 1st 180 bp of 5' u…

   Promoter

   Available since

   Oct. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

8. pET22b-mEB3-mCherry-6xHis

   Plasmid

   #130985

   Purpose

   Bacterial expression of mouse EB3-mCherry-6xHis for protein purification.

   Depositor

   Anne Straube

   Article

   Roth et al J Cell Sci. 2018 Oct 31;132(4). pii: jcs

   Insert

   EB3-mCherry-6xHis (Mapre3 Mouse)
   

   Use

   Tags

   mCherry-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET32a-HD39Q

   Plasmid

   #11514

   Depositor

   Pamela Bjorkman

   Article

   Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

   Insert

   huntingtin, exon 1, 39 glutamines (HTT Human)
   

   Use

   Tags

   His, His (out of frame), and Trx

   Expression

   Bacterial

   Mutation

   huntingtin exon 1 with 39 glutamines, vector m…

   Promoter

   Available since

   April 28, 2006

   Availability

   Academic Institutions and Nonprofits only

10. 2xmyc-RAB35

    Plasmid

    #164632

    Purpose

    Expresses RAB35 in mammalian cells with a 2xmyc tag

    Depositor

    Mark Cookson

    Article

    Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

    Insert

    RAB35 (RAB35 Human)
    

    Use

    Tags

    2xmyc

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. DN1-Gli3 in AINGpCAGGS

    Plasmid

    #121970

    Purpose

    medium Gli3 transcriptional activator

    Depositor

    James Briscoe

    Article

    Stamataki et al Genes Dev. 2005 Mar 1;19(5):626-41. doi:

    Insert

    DN2Gli3 (GLI3 Human)
    

    Use

    Chick electroporation

    Tags

    5xmyc

    Expression

    Mammalian

    Mutation

    N terminus deletion (nt1-864)

    Promoter

    Available since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHAGE2 mCherry-Rtn4HD

    Plasmid

    #86685

    Purpose

    Lentivirus to stably express fluorescent human protein in mammalian cells

    Depositor

    Tom Rapoport

    Article

    Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

    Insert

    Rtn4 homology domain (RTN4 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    homology domain only

    Promoter

    CMV

    Available since

    Feb. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1 E2F1 HA(Δ2-126) (HindIII- BamHI- EcoRI)

    Plasmid

    #70668

    Purpose

    Human mutant of E2F1 lacking amino acids 2 to 126

    Depositor

    Maria Alvarado-Kristensson

    Article

    Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

    Insert

    E2F1 lacking residues 2 to 126 (E2F1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    lacks amino acids 2-126

    Promoter

    cmv

    Available since

    Nov. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pHAGE2 GFP-Rtn4HD

    Plasmid

    #86686

    Purpose

    Lentivirus to stably express fluorescent human protein in mammalian cells

    Depositor

    Tom Rapoport

    Article

    Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

    Insert

    Rtn4 homology domain (RTN4 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    homology domain only

    Promoter

    CMV

    Available since

    Feb. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. actc1b-mKate2-SERCA1

    Plasmid

    #109498

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to sarcoplasmic/endoplasmic calcium ATPase 1

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    SERCA1 (atp2a1 Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    mKate2

    Expression

    Mutation

    Please see Depositor Comments

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRetroX-Tight-Pur-Trim69 Full length, isoform A (codon optimized)

    Plasmid

    #199566

    Purpose

    Stable and dox. inducible expression of Trim69 Full length, isoform A (codon optimized) after retroviral-mediated gene transfer

    Depositor

    Yuxin Song, Andrea Cimarelli

    Article

    Song et al Proc Natl Acad Sci U S A. 2022 Oct 25;11

    Insert

    Trim69 Full length, isoform A (codon optimized) (TRIM69 Human)
    

    Use

    Retroviral; Allows for puromycin selection

    Tags

    Flag

    Expression

    Mutation

    codon optimized

    Promoter

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV_SEPT9_i1 D1-25-msfGFP

    Plasmid

    #180333

    Purpose

    mammalian expression of human SEPT9_i1 D1-25 fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

    Insert

    SEPTIN9_v1 (SEPTIN9 Human)
    

    Use

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    SEPT9_i1 D1-25

    Promoter

    CMV

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSpCas9_BB_2A-GFP_MAPRE3-gRNA#2

    Plasmid

    #107730

    Purpose

    Knock out of EB3 in human cells by CRISPR/Cas9

    Depositor

    Torsten Wittmann

    Article

    van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

    Insert

    MAPRE3 gRNA #2 (targets Exon 1) (MAPRE3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSpCas9_BB_2A-GFP_MAPRE3-gRNA#1

    Plasmid

    #107729

    Purpose

    Knock out of EB3 in human cells by CRISPR/Cas9

    Depositor

    Torsten Wittmann

    Article

    van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

    Insert

    MAPRE3 gRNA #1 (targets Exon 1) (MAPRE3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLAP-BUBR1-dKARD

    Plasmid

    #114053

    Purpose

    transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

    Depositor

    Geert Kops

    Article

    Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

    Insert

    BUBR1-dKARD (BUB1B Human)
    

    Use

    Tags

    YFP-TEV-S- Human LAP

    Expression

    Mammalian

    Mutation

    C2823A and G2826A (siRNA-resistance): AGATACTAGCT…

    Promoter

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCR3-vsv-GCN4-dCEN-INCENP

    Plasmid

    #108510

    Purpose

    expression of vsv-GCN4-dCEN-INCENP

    Depositor

    Susanne Lens

    Article

    van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

    Insert

    GCN4-dCEN-INCENP (INCENP Human)
    

    Use

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    replace CEN box for GCN4 dimerazation domain

    Promoter

    CMV

    Available since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBrain-GFP-TACC3KDP(S558A)-shTACC3

    Plasmid

    #59357

    Purpose

    Expresses GFP-tagged human TACC3 (RNAi-resistant) with S558A substitution and knocks down endogenous TACC3 via shRNA.

    Depositor

    Stephen Royle

    Article

    Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10

    Insert

    Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
    

    Use

    RNAi

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Silent mutations in amino acids 30-33 to confer s…

    Promoter

    CMV

    Available since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRC/RSV-rat Agt

    Plasmid

    #122104

    Purpose

    Expression of angiotensinogen

    Depositor

    John Chan

    Article

    Zhang et al J Am Soc Nephrol. 2002 Feb;13(2):302-12.

    Insert

    angiotensinogen (Agt Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    RSV promoter

    Available since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSpCas9_BB_2A-GFP_MAPRE3-gRNA#3

    Plasmid

    #107731

    Purpose

    Knock out of EB3 in human cells by CRISPR/Cas9

    Depositor

    Torsten Wittmann

    Article

    van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

    Insert

    MAPRE3 gRNA #3 (targets Exon 1) (MAPRE3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCMV_SEPT2 NCmut-mApple

    Plasmid

    #180321

    Purpose

    mammalian expression of human SEPT2 NCmut fused to monomeric Apple

    Depositor

    Manos Mavrakis

    Article

    Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

    Insert

    SEPTIN2 (SEPTIN2 Human)
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    SEPT2 F20D, V27D

    Promoter

    CMV

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCMV_SEPT9_i1-i5-msfGFP

    Plasmid

    #180332

    Purpose

    mammalian expression of human SEPT9_i1-i5 fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

    Insert

    SEPTIN9_v7 (SEPTIN9 Human)
    

    Use

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    SEPT9_i1-i5

    Promoter

    CMV

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. EGFP-Bin1b

    Plasmid

    #176014

    Purpose

    Mammalian expression of zebrafish EGFP-Bin1b. Parton lab clone HYG

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    Bin1b (bin1b Zebrafish)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLAP-MIS12-KARD

    Plasmid

    #114057

    Purpose

    transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

    Depositor

    Geert Kops

    Article

    Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

    Insert

    MIS12-KARD (BUB1B Human)
    

    Use

    Tags

    YFP-TEV-S- Human LAP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAD120-CHIP-G132N-myc-His

    Plasmid

    #236053

    Purpose

    Mammalian expression of human CHIP with the synthetic G132N substitution and C-terminal myc-His tag

    Depositor

    Jonathan Schisler

    Article

    Stewart et al bioRxiv 2025.02.17.637997

    Insert

    CHIP-G132N (STUB1 Human)
    

    Use

    Tags

    myc-His

    Expression

    Mammalian

    Mutation

    G132N

    Promoter

    CMV

    Available since

    Aug. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAD000-CHIP-K30A-myc-His

    Plasmid

    #236052

    Purpose

    Mammalian expression of human CHIP with the synthetic K30A substitution and C-terminal myc-His tag

    Depositor

    Jonathan Schisler

    Article

    Stewart et al bioRxiv 2025.02.17.637997

    Insert

    CHIP-K30A (STUB1 Human)
    

    Use

    Tags

    myc-His

    Expression

    Mammalian

    Mutation

    K30A

    Promoter

    CMV

    Available since

    Aug. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge