We narrowed to 13,482 results for: cas9 genes

1. pScI_dCas9-CDA_J23119-sgRNA

   Plasmid

   #108550

   Purpose

   Bacterial Target-AID vector with sgRNA

   Depositor

   Akihiko Kondo

   Article

   Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

   Inserts

   dCas9-PmCDA1
   Streptococcus pyogenes sgRNA
   

   Use

   Tags

   Flag

   Expression

   Bacterial

   Mutation

   D10A and H840A for SpCas9

   Promoter

   J23119 and lambda OR

   Available since

   Sept. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. 3xFLAG-dCas9/p-bacteria

   Plasmid

   #64325

   Purpose

   Expresses 3xFLAG-dCas9 in E. coli in a tetracycline-dependent manner for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Fujita et al BMC Res Notes. 2018 Jun 14;11(1):387. do

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR; Tetracycline-inducible

   Tags

   3xFLAG

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   pLtetO-1

   Available since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. SpCas9-CBE6a-V106W

   Plasmid

   #215824

   Purpose

   Express CBE6a-V106W (with SpCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

   Insert

   CBE6a V106W-SpCas9-2xUGI (cas9 )
   

   Use

   In vitro transcription template

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. PX458-3xHA-FnCas9

   Plasmid

   #130969

   Purpose

   3xHA tagged Cas9 from F. novicida with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Proc Natl Acad Sci U S A. 2019 Oct 15;11

   Insert

   FnCas9
   

   Use

   CRISPR

   Tags

   3xHA-NLS and NLS-T2A-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   Nov. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. PX458-3xHA-en31FnCas9ABEmax8.17d

   Plasmid

   #201956

   Purpose

   Mammalian expression plasmid of en31FnCas9ABEmax8.17d base editor with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

   Insert

   bpNLS-en31FnCas9ABEmax8.17d-bpNLS-3xHA-T2A-EGFP
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX165-LZ3 Cas9

   Plasmid

   #140561

   Purpose

   Expresses LZ3 Cas9 in mammalian cells

   Depositor

   Feng Zhang

   Article

   Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Mutation

   N690C T769I G915M N980K

   Promoter

   Available since

   May 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. p-dCas9-VP64-Hygro

   Plasmid

   #104405

   Purpose

   transient expression of dCas9-VP64 fusion protein

   Depositor

   Stephan Beck

   Insert

   VP64
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. SpCas9-CBE6a-IVT

   Plasmid

   #215833

   Purpose

   Template for in vitro transcription of CBE6a (with SpCas9)

   Depositor

   David Liu

   Article

   Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

   Insert

   CBE6a-SpCas9-2xUGI
   

   Use

   In vitro transcription template

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSpCas9(BB)-T2A-HygR

   Plasmid

   #118153

   Purpose

   SpCas9 expression vector

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   hSpCas9-T2A-HygR
   

   Use

   CRISPR

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and U6

   Available since

   March 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti6.3-SaCas9-BlastR

    Plasmid

    #220966

    Purpose

    Expresses human codon-optimized SaCas9

    Depositor

    Hyongbum Kim

    Article

    Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

    Insert

    SaCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-Neo_CAG-Cas9

    Plasmid

    #86698

    Purpose

    Cas9 expression vector for targeting to the AAVS1 locus

    Depositor

    Ludovic Vallier

    Article

    Bertero et al Development. 2016 Dec 1;143(23):4405-441

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCag

    Available since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-dCas9-D3A

    Plasmid

    #78256

    Purpose

    Expresses dead Cas9 (dCas9) fused to DNMT3A catalytic domain (CD) under the control of the CMV promoter

    Depositor

    Grant Challen

    Article

    McDonald et al Biol Open. 2016 May 11. pii: bio.019067.

    Insert

    DNMT3A CD aa 598-912 (DNMT3A Human)
    

    Use

    CRISPR

    Tags

    Flag epitope tag

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-Cas9-NRCH

    Plasmid

    #136926

    Purpose

    Expresses Cas9-NRCH in mammalian cells

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    Cas9-NRCH
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    see manuscript

    Promoter

    CMV

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAW90.dCas9-YY1

    Plasmid

    #104373

    Purpose

    Expresses dCas9-YY1

    Depositor

    Richard Young

    Article

    Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

    Insert

    dCas9-YY1
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. PX458-3xHA-en1FnCas9

    Plasmid

    #201947

    Purpose

    Mammalian expression plasmid of enhanced activity FnCas9 variant en1FnCas9 with T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

    Insert

    3xHA-NLS-en1FnCas9-T2A-EGFP
    

    Use

    CRISPR

    Tags

    3xHA, NLS, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    E1369R on FnCas9

    Promoter

    Cbh

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. eSpCas9(1.1)_No_FLAG_LMNA_G2

    Plasmid

    #178090

    Purpose

    Expresses the LMNA G2 sgRNA in combination with FLAGless eSpCas9(1.1). This vector can be used in combination with LMNA_mScarlet-I_Donor to tag LMNA with mScarlet-I. pX330-like plasmid.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    LMNA G2 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAaePUb::Cas9-2A-Neo

    Plasmid

    #176680

    Purpose

    Expression of Drosophila codon optimized spCas9 under Ae. aegypti Polyubiquitin promoter (AAEL003888)

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Insert

    spCas9; NeoR (aminoglycoside phosphotransferase from Tn5)
    

    Use

    Crispr

    Tags

    Expression

    Insect

    Mutation

    Drosophila codon optimized

    Promoter

    Ae. aegypti poly-ubiquitin (AAEL003888)

    Available since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pINDUCER dCas9-TET1CDMut

    Plasmid

    #101920

    Purpose

    The dCas9-TET1CDMut fusion protein is cloned into the pINDUCER lentiviral backbone (Addgene plasmid# 46948). The TET1 catalytic domain is mutated to abolish catalytic function.

    Depositor

    Danwei Huangfu

    Article

    Verma et al Nat Genet. 2018 Jan;50(1):83-95. doi: 10

    Insert

    dCas9-TET1CD Mut
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Mutated TET1 catalytic domain

    Promoter

    Available since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTO135_pENO1-CaCas9-tCyc1

    Plasmid

    #171103

    Purpose

    Expresses Cas9 in Candida auris

    Depositor

    Teresa O'Meara

    Article

    Santana et al Nat Commun. 2021 Dec 10;12(1):7197. doi:

    Insert

    CaCas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ENO1

    Available since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. lenti-SpCas9 blast

    Plasmid

    #104997

    Purpose

    This lentiviral construct delivers hSpCas9 and blasticidin S resistance.

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Insert

    KpnI-XhoI-BsrGI-NheI
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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