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pCDNA3 Flag Bmf (S74D)

Plasmid

#24259

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

Bmf (S87D) (BMF Human)

Tags

Flag

Expression

Mammalian

Mutation

changed Serine 74 to Aspartic Acid

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag Bmf (S74A)

Plasmid

#24258

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

Bmf (S87A) (BMF Human)

Tags

Flag

Expression

Mammalian

Mutation

changed Serine 74 to Alanine

Available Since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
PX458_MBD1_iso1_1

Plasmid

#86303

Purpose

Encodes gRNA for 3' target of human MBD1_iso1

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against MBD1_iso1 (MBD1 Human)

Use

CRISPR

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pVenus-N1 cyclin B1 L45A (1324)

Plasmid

#61851

Purpose

mammalian expression of cyclin B1 L45 A mutant fused to Venus

Depositor

Jonathon Pines

Article

Matsusaka et al Biol Open. 2014 Sep 12;3(10):904-12. doi

Insert

cyclin B1 (CCNB1 Human)

Tags

Venus

Expression

Mammalian

Mutation

L45A

Promoter

CMV

Available Since

Feb. 4, 2015

Availability

Academic Institutions and Nonprofits only
pIW069

Plasmid

#134133

Purpose

Retroviral expression of GFP-Ska1

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

GFP-human Ska1 CRISPR/Cas9 hardened (SKA1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

CRISPR resistant

Available Since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag BimL (S58D)

Plasmid

#24239

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (S58D) (BCL2L11 Human)

Tags

Flag

Expression

Mammalian

Mutation

Changed serine 58 to aspartic acid

Available Since

May 10, 2010

Availability

Academic Institutions and Nonprofits only
pSAH0016

Plasmid

#79824

Purpose

Dual selection plasmid with lac operator, selection for binding with spectinomycin, for non-binding with chloramphenicol

Depositor

Katja Arndt

Article

Hoffmann et al Nucleic Acids Res. 2016 Jun 2;44(10):e95

Inserts

cat
aadA

Promoter

AATTCAAAAAAATGCTTGACAATTAATCATCGGCTCGTATAATGTGTGG…

Available Since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pCSDest2-SpCas9-MT3-NLS-3XHA-NLS

Plasmid

#69223

Purpose

Expresses R1335K mutant SpCas9 in mammalian cell

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

SpCas9

Use

CRISPR

Tags

NLS-3XHA-NLS

Expression

Mammalian

Mutation

Arginine 1335 is changed to Lysine

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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Rae1-pDONR221

Plasmid

#124240

Purpose

Gateway entry clone of Drosophila Rae1 cDNA.

Depositor

Peter Vilmos

Article

Kristo et al Biochim Biophys Acta Mol Cell Res. 2017

Insert

Drosophila Rae1 (Rae1 Fly)

Expression

Insect

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pFETCh_ZNF652

Plasmid

#86270

Purpose

Donor vector for 3' FLAG tag of human ZNF652

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZNF652 homology arms (ZNF652 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mETV3

Plasmid

#33374

Depositor

Peter Murray

Article

El Kasmi et al J Immunol. 2007 Dec 1;179(11):7215-9.

Insert

Etv3 (Etv3 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 20, 2011

Availability

Academic Institutions and Nonprofits only
pMX Vav1 Y3F/SH2* (R696K) uGFP

Plasmid

#14565

Depositor

Joan Brugge

Article

Wilsbacher et al Cell Commun Signal. 2006 May 18;4:5. doi

Insert

Vav1 (VAV1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Y3F/SH2* (R696K)

Available Since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
pAdTrackCMV-PSM-Rb

Plasmid

#37107

Depositor

Joseph Nevins

Article

Angus et al Oncogene. 2012 Jan 16. doi: 10.1038/onc.

Insert

Rb (RB1 Human)

Use

Adenoviral

Expression

Mammalian

Mutation

aa 379-928 only; large pocket of human Rb with aa…

Promoter

CMV

Available Since

May 31, 2012

Availability

Academic Institutions and Nonprofits only
pGex2t SadBs

Plasmid

#66882

Purpose

Bacterial expression vector encoding N-terminally GST-tagged short isoform of human SadB

Depositor

Maria Alvarado-Kristensson

Article

Eklund et al J Biol Chem. 2014 Aug 1;289(31):21360-73

Insert

SadB (BRSK1 Human)

Tags

GST

Expression

Bacterial

Available Since

June 25, 2015

Availability

Academic Institutions and Nonprofits only
pCAE-MIBP1

Plasmid

#51873

Purpose

Expresses full length rat MIBP1 (cloned in pCAGGS)

Depositor

Kenshi Hayashi, Tomoko Tahira

Article

Fukuda et al J Biochem. 2002 Mar;131(3):349-57.

Insert

MIBP1 (Hivep2 Rat)

Tags

none

Expression

Mammalian

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only
pMX Vav1 Y3F/CSH3* (P833L) uGFP

Plasmid

#14566

Depositor

Joan Brugge

Article

Wilsbacher et al Cell Commun Signal. 2006 May 18;4:5. doi

Insert

Vav1 (VAV1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Y3F/CSH3* (P833L)

Available Since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
RAB14 3'UTR D1

Plasmid

#61490

Purpose

luciferase reporter containing RAB14 3' UTR with the first predicted MiR144 binding site deleted

Depositor

Curt Civin

Article

Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111

Insert

RAB14 3' UTR (RAB14 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

contains position 837-1637 (numbering relative to…

Promoter

PGK

Available Since

Jan. 9, 2015

Availability

Academic Institutions and Nonprofits only
pFA6a-kanMX6-Purg1-GST

Plasmid

#39352

Depositor

Jurg Bahler

Article

Watt et al PLoS One. 2008 Jan 16;3(1):e1428.

Insert

urg1 promoter (urg1, urg1 Fission Yeast)

Use

Yeast genomic targeting

Tags

GST and kanMX6 module - reverse orientation

Available Since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only
pGEX2T merlin 308-579

Plasmid

#11632

Depositor

Vijaya Ramesh

Article

Gonzalez-Agosti et al J Biol Chem. 1999 Nov 26. 274(48):34438-

Insert

merlin (NF2 Human)

Tags

GST

Expression

Bacterial

Mutation

Carboxy-terminal common domain of merlin.

Available Since

May 3, 2006

Availability

Academic Institutions and Nonprofits only
pKDsgRNA-trmI

Plasmid

#89955

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting trmI.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

trmI gRNA

Expression

Bacterial

Promoter

pTet

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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