We narrowed to 14,460 results for: ung

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fck-Jaws-KGC-GFP-ER2

Plasmid

#65012

Purpose

Lentivirus-mediated expression of Jaws under the CaMKII promoter.

Depositor

Edward Boyden

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Jaws-KGC-GFP-ER2

Use

Lentiviral

Tags

ER2, GFP, and KGC

Expression

Mammalian

Mutation

K200R W214F

Promoter

CaMKII

Available Since

July 14, 2015

Availability

Academic Institutions and Nonprofits only

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pGAPTrap-eGFP-IRESMygro

Plasmid

#82503

Purpose

Vector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH. Hygromycin B resistance is encoded by the Mygro gene.

Depositor

Ed Stanley

Article

Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

Insert

eGFP-IRESMygro

Use

Synthetic Biology

Expression

Mammalian

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLEX301-RFP-GGA3 NGAT

Plasmid

#162031

Purpose

Expresses NGAT sequence of GGA3

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Insert

GGA3 NGAT (GGA3 Human)

Use

Lentiviral

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-NFIB-T2A-miRFP670

Plasmid

#187222

Purpose

Expresses human NFIB and miRFP670 via T2A linker under control of a CMV promoter.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Insert

Nuclear Factor I B (NFIB Human)

Tags

Inserted T2A-miRFP670 at 3' terminal of MCS.

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR-HLA-B0702-His

Plasmid

#104849

Purpose

HLA-B0702 his tagged

Depositor

Sébastien Walchli

Article

Lampen et al Immunohorizons. 2018 Jul 2;2(6):172-184.

Insert

major histocompatibility complex, class I, B (HLA-B Human)

Use

Entry vector for gateway system

Tags

his

Promoter

none

Available Since

Dec. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2plus-mVenus1-155-I152L-GGGS

Plasmid

#162613

Purpose

Allows for transcription of improved control mVenus I152L fluorophore half for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

mVenus

Expression

Mammalian

Mutation

1-155-I152L

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003 sgKEAP1-1

Plasmid

#74985

Purpose

sgKEAP1-1, puromycin selection

Depositor

William Hahn

Article

Krall et al Elife. 2017 Feb 1;6:e18970. doi: 10.7554

Insert

KEAP1 (KEAP1 Human)

Use

CRISPR

Expression

Mammalian

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pFastBac (GST-SNAP-Shp2)

Plasmid

#177897

Purpose

Baculo expression of GST tag fused to SNAP tag and human Shp2

Depositor

Ron Vale

Article

Hui et al Science. 2017 Mar 31;355(6332):1428-1433

Insert

Shp2 (PTPN11 Human)

Tags

TEV-GST-PreScission cut site-SNAP tag

Expression

Insect

Promoter

Polyhedrin

Available Since

Dec. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flt3-Y591F/Y919F

Plasmid

#74497

Purpose

Overexpression of FLT3 Y591F/Y919F mutant in mammalian cells

Depositor

Lars Rönnstrand

Article

Kazi et al J Biol Chem. 2012 Oct 19;287(43):36509-1

Insert

FLT3 (FLT3 Human)

Expression

Mammalian

Mutation

Y591F/Y919F, T227M

Promoter

CMV

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
pBabe-TetCMV-puro-mVenus-PA-Rac1-C450M

Plasmid

#22034

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

mVenus-PA-Rac1-C450M (RAC1 Human, Avena sativa (oat))

Use

Retroviral

Expression

Mammalian

Mutation

The LOV domain contains the mutation C450M.Rac1 s…

Available Since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only
pET-NCBD + ACTR

Plasmid

#99335

Purpose

Bacterial coexpression of CBP NCBD and NCoA3 binding domains

Depositor

Peter Wright

Article

Demarest et al Nature. 2002 Jan 31;415(6871):549-53.

Inserts

CBP NCBD (2059-2117) (CREBBP Mouse)
SRC3 (1023-1093) (NCOA3 Human)

Expression

Bacterial

Promoter

T7

Available Since

Sept. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pGL

Plasmid

#119952

Purpose

Expresses GPPS and LimS, for the production of GPP and (S)-limonene, in Escherichia coli.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.102

Inserts

gpps
limS

Expression

Bacterial

Mutation

N-terminal truncation of 56 amino acid residues (…

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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p3xFLAG CMV14 / BRAF-D594N

Plasmid

#131713

Purpose

Mammalian Expression of Flag tagged BRAF

Depositor

Jacques De Grève

Article

Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

Insert

BRAF (BRAF Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

D594N

Promoter

CMV

Available Since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pLEX304-GFP-v3 IQSEC1 WT

Plasmid

#162018

Purpose

Expression of IQSEC1 splice variant 3

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Insert

IQSEC1 (IQSEC1 Human)

Use

Lentiviral

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX304-GFP-v4 IQSEC1 WT

Plasmid

#162019

Purpose

Expression of IQSEC1 splice variant 4

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Insert

IQSEC1 (IQSEC1 Human)

Use

Lentiviral

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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PLUNC-bio-His

Plasmid

#53428

Purpose

Expresses full-length Palate lung and nasal epithelium clone protein precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

PLUNC (BPIFA1 Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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U6-hGRIN2B-CAG-ps-SaCas9

Plasmid

#102853

Purpose

A single-chain light-controllable dSaCas9 with pdDronpa1 domains for hGRIN2B gene editing

Depositor

Michael Lin

Article

Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

Insert

sa-hGRIN2B-sgRNA; dSaCas9; pdDronpa1 (GRIN2B Human, S. aureus, Synthetic)

Use

CRISPR

Tags

3X Flag and NLS

Expression

Mammalian

Promoter

U6 promoter;

Available Since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

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Myc-Nix-delta TM

Plasmid

#100755

Purpose

Mammalian expression of Nix dominant negative

Depositor

Joseph Gordon

Article

Mughal et al Cell Death Differ. 2018 Mar 6. pii: 10.1

Insert

Nix-_TM (BNIP3L Human)

Tags

Myc

Expression

Mammalian

Mutation

TM deletion

Promoter

CMV

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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MBP-rPKM2 WT

Plasmid

#107158

Purpose

MBP-rPKM2 WT for production and purification of rat PKM2 WT

Depositor

Jesse Rinehart

Article

Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

Insert

MBP-tagged rat pyruvate kinase M2 isoform (Pkm Rat)

Tags

C-term 6-his and N-term MBP

Expression

Bacterial

Promoter

PBAD

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PRKD1

Plasmid

#116778

Purpose

Lentiviral expression of PRKD1

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PRKD1 (PRKD1 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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