2,162 results
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Plasmid#140639PurposeHeat shock promoter driven expression of human APOB48-mCherry. The construct has tol2 elements, and is designed to use in zebrafish.DepositorUseZebrafish expressionTagsmCherryExpressionMutationAPOB48: amino acid 1-2180Promoterhsp70lAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only
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pCS2-Opto-Acvr1ba-P2A-Acvr2ba
Plasmid#232640PurposeOptogenetic Nodal activators from Sako et al. 2016 (Cell Reports) separated by a P2A peptide for singe-transcript expressionDepositorInsertAcvr1ba + VfLOV domain and Acvr2ba + VfLOV domain
UseTagsExpressionMutationPromoterAvailable sinceApril 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSCAC-69
Plasmid#31241PurposeMitochondrial localization of EGFP, for real-time imaging of mitochondria in transgenic zebrafishDepositorInsertmitochondrial localization enhanced green fluorescent protein
UseTagsExpressionMutationPromoterAvailable sinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
p5E-2.3k-fabp2
Plasmid#84927Purpose–2.3kb promoter of zebrafish fabp2/ifabp gene in Gateway 5’ Entry vectorDepositorInsert–2.3kb promoter of zebrafish fabp2/ifabp gene (fabp2 Zebrafish)
UseTagsNOExpressionBacterialMutationPromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTol2p2A--3mnx1:mTagBFP-NS-MCS
Plasmid#78250PurposeCan be used to subclone in gene of interest into blue motor neuron expression vector.DepositorInsertsUseZebrafish motor neuron expressionTagsExpressionMutationPromoter-3mnx1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-gfap(Intron1/5'/Exon1-zebrafish)
Plasmid#39761DepositorInsertzebrafish gfap promoter- EGFP (gfap Zebrafish)
UseZebrafish expressionTagsExpressionMutationPromotergfapAvailable sinceAug. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-actinb-kcna1a-IRES-EGFP
Plasmid#164965PurposeTol2 construct: actinb (actb2) promoter driven zebrafish kcna1a and EGFPDepositorInsertkcna1a (kcna1a Zebrafish)
UseTol2 transposon destination vectorTagsIRES-EGFPExpressionMutationPromoteractinb (actb2) promoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2+cPLA2-mKate2
Plasmid#162068PurposeUsed to provide a steady-state readout of cPLA2 activation in live cells. Amino-terminally fused to mKate2 for red fluorescence.DepositorInsertcytosolic phospholipase a2 (pla2g4aa Zebrafish)
UseAvian, xenopus, zebrafishTagsmKate2ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestGFP_RNAseH1
Plasmid#65784Purposeexpresses GFP-tagged RNAseH1 in mammalian cellsDepositorInsertRNaseH1 (RNASEH1 Zebrafish, Human)
UseTagsGFP and V5ExpressionMammalianMutationPromoterCMV, 2x TetAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
p5'Entry-lck-7.4kb
Plasmid#58891Purposeprovides zebrafish lck promoter (7.4 kb) for GateWay construction of Tol2 transgenesDepositorInsertzebrafish lymphocyte-specific protein tyrosine kinase (lck) promoter, 7.4 kb
UseGateway 5' entry cloneTagsExpressionMutationmutated single nucleotide (T-->C, 1874th nucle…Promoterlymphocyte-specific protein tyrosine kinase (lck)…Available sinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
p3E_he1a:eYFP
Plasmid#113879Purposep3E entry vector for Three Multisite GatewayDepositorInserthe1a (he1.1 Zebrafish)
UseZebrafish danio expressionTagseYFPExpressionMutationPromoterAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pME-Trpa1b-2A-mCherry
Plasmid#106291PurposeGateway middle entry construct containing zebrafish Trpa1b, 2A self-cleaving peptide and mCherryDepositorInsertTrpa1b (trpa1b Zebrafish)
UseGateway middle entryTags2A-mCherryExpressionMutationPromoterAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
p5E-3x125bp-mnx1
Plasmid#169043Purpose5' entry vector for Gateway cloning for expression in spinal motor neurons using 3 tandem repeats of the zebrafish mnx1 enhancer.DepositorInsertMnx1 enhancer (3x) (mnx1 Zebrafish)
UseGateway entry cloning vectorTagsExpressionMutationPromoter3xMnx1Available sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVC-Tol2-Pax3a_enh5-E1b:eGFP-Tol2
Plasmid#119061PurposeFluorescent vector with zebrafish pax3a enhancer 5; Tol2 versionDepositorInsertpax3a_enh5-E1b:eGFP
UseTagsExpressionBacterialMutationPromoterE1b minimal promoterAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pME Integrin beta 1b
Plasmid#105976Purposeentry clone for gateway cloning, Full length Integrin beta 1b without stopDepositorInsertIntegrinbeta1b
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-FLAG3-drAgo2DY-EF
Plasmid#221716PurposeExpression of zebrafish Ago2 with D651E and Y680F mutations and N-terminal 3xFLAG tag in zebrafish embryos.DepositorInsertdrAgo2DY-EF (ago2 Zebrafish)
UseFish expressionTags3xFLAGExpressionMutationD651E and Y680F in active sitePromotersimian CMV IE94Available sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-FLAG3-drAgo2
Plasmid#221712PurposeExpression of zebrafish Ago2 with N-terminal 3xFLAG tag in zebrafish embryos.DepositorInsertdrAgo2 (ago2 Zebrafish)
UseFish expressionTags3xFLAGExpressionMutationPromoterAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST mfap4:turquoise2
Plasmid#135218PurposeCyan tagged macrophagesDepositorInsertmfap4 promoter and mTurquoise2
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
punc503GFP
Plasmid#64021Purpose503-bp promoter of zebrafish unc-45b drives muscle-specific GFP expressionDepositorInsertunc-45b: GFP (unc45b Zebrafish)
UseGatewayTagsExpressionMutationPromoter503-bp promoter of zebrafish unc-45bAvailable sinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
Zed_vector_tol2_-2.1prox1a_1:EGFP_XCA:DsRed2
Plasmid#218205PurposeZebrafish -2.1prox1a enhancer reporter in the lymphatic valve from 3 dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows the high background typical of the ZED vectorDepositorInsert-2.1prox1a
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only