We narrowed to 5,300 results for: pcDNA3.1+

1. pcDNA3.1alphaFEM1CalphaFLAG

   Plasmid

   #236462

   Purpose

   transient overexpression of FEM1C in mammalian cells

   Depositor

   Michele Pagano

   Article

   Dankert et al Cell Cycle. 2017 Mar 19;16(6):556-564. d

   Insert

   FEM1C (FEM1C Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCDNA3.1_WiChR_TS_mScarlet_ER

   Plasmid

   #195190

   Purpose

   High efficient, K+ selective channelrhodopsin for the inhibition of excitable cells

   Depositor

   Peter Hegemann, Johannes Vierock

   Article

   Vierock et al Sci Adv. 2022 Nov 16:eadd7729.

   Insert

   Wobblia lunata inhibitory Channelrhodopsin
   

   Use

   Tags

   ER-export sequence, Kir2.1 trafficking motif, and…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1_rSERT_WT

   Plasmid

   #190743

   Purpose

   For expression of wild-type rat SERT in mammalian cells

   Depositor

   Gary Rudnick

   Article

   Singh et al bioRxiv 2022.06.13.495991

   Insert

   SLC6A4 Serotonin Transporter (Slc6a4 Rat)
   

   Use

   Tags

   FLAG, His6, and c-myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. HcKCR2_mCherry_pcDNA3.1

   Plasmid

   #177337

   Purpose

   Expresses KCR2 in mammalian cells

   Depositor

   John Spudich

   Article

   Govorunova et al Nat Neurosci. 2022 Jun 20. pii: 10.1038/

   Insert

   HcKCR2
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   mammalian codon-adapted

   Promoter

   Available since

   Dec. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1_PsChR2_EYFP

   Plasmid

   #69057

   Purpose

   High-efficiency blue-shifted cation channelrhodopsin

   Depositor

   John Spudich

   Article

   Govorunova et al J Biol Chem. 2013 Oct 11;288(41):29911-2

   Insert

   PsChR2
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1_ionRD

   Plasmid

   #172931

   Purpose

   Ionic strength FRET sensor with mCerulean3 and mCitrine for mammalian cell expression.

   Depositor

   Arnold Boersma

   Article

   Liu et al ACS Chem Biol. 2017 Oct 20;12(10):2510-2

   Insert

   IonicStrengthSensorRD
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1-HA-14-3-3 ϵ

   Plasmid

   #48797

   Purpose

   expresses human myc-14-3-3ϵ in mammalian cells

   Depositor

   Huda Zoghbi

   Article

   Chen et al Cell. 2003 May 16;113(4):457-68.

   Insert

   YWHAE 14-3-3 ϵ (YWHAE Human)
   

   Use

   Tags

   haemagglutinin

   Expression

   Mammalian

   Mutation

   Promoter

   cmv

   Available since

   June 4, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3.1hSIRT3_H248Y_HA

   Plasmid

   #24492

   Depositor

   Eric Verdin

   Article

   Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

   Insert

   Human Sirtuin 3 catalytically inactive mutant (SIRT3 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   H248Y

   Promoter

   Available since

   April 23, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1hSIRT4_HA

   Plasmid

   #24482

   Depositor

   Eric Verdin

   Article

   Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

   Insert

   Sirtuin 4 (SIRT4 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 23, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1_ionRE

    Plasmid

    #172932

    Purpose

    Ionic strength FRET sensor with mCerulean3 and mCitrine for mammalian cell expression.

    Depositor

    Arnold Boersma

    Article

    Liu et al ACS Chem Biol. 2017 Oct 20;12(10):2510-2

    Insert

    IonicStrengthSensorRE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1.H1-NP

    Plasmid

    #134367

    Purpose

    Nanoluc complementation assay. Expression of histamine receptor H1 fused at C termimus with Natural peptide (NP) of NanoLuc. Addition of the Flag epitope at N terminus of H1 receptor.

    Depositor

    Julien Hanson

    Article

    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

    Insert

    H1-NP (HRH1 Human)
    

    Use

    Tags

    Flag and natural peptide of nanoluciferase

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    T7

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1_27F3_hFc

    Plasmid

    #199619

    Purpose

    Encodes DARPin-hFc 27F3 for mammalian expression and column purification

    Depositor

    Shiva Tyagarajan

    Article

    Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

    Insert

    DARPin-hFc 27F3
    

    Use

    Tags

    hFc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1_hMed25

    Plasmid

    #206958

    Purpose

    The ORF of human Med25 cDNA amplified from the RT human liver RNA was cloned into pGEMTE vector and then subclone at XhoI/XbaI sites of the pcDNA3.2(+), the mammalian expression vector.

    Depositor

    Catharine Ross, Reza Zolfaghari

    Insert

    Homo sapiens mediator complex subunit 25 (MED25), transcript variant 1, mRNA (MED25 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1_hE2F7mg

    Plasmid

    #73073

    Purpose

    human E2F7 minigene (exons 11,12,13/introns cassette)

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

    Insert

    E2F7 (E2F7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    partial gene, exons 11,12,13 spaced by introns

    Promoter

    CMV

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1dsRluc

    Plasmid

    #68053

    Purpose

    expresses destabilized Renilla Luciferase

    Depositor

    Christopher Sullivan

    Article

    Seo et al J Virol. 2008 Oct;82(20):9823-8. doi: 10

    Insert

    Renilla luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 11, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

16. HfACR1_EYFP_pcDNA3.1

    Plasmid

    #161026

    Purpose

    Expresses strongly red-shifted HfACR1 in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al Proc Natl Acad Sci U S A. 2020 Sep 15;11

    Insert

    HfACR1
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCDNA3.1_CoumRS

    Plasmid

    #64882

    Purpose

    Expresses Coumarinyl- tRNA synthetase

    Depositor

    Sebastián Brauchi

    Article

    Steinberg et al bioRxiv, 10.1101/065227

    Insert

    Coum_RS
    

    Use

    Tags

    Flag

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. A1ACR1_EYFP_pcDNA3.1

    Plasmid

    #161025

    Purpose

    Expresses strongly red-shifted A1ACR1 in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al Proc Natl Acad Sci U S A. 2020 Sep 15;11

    Insert

    A1ACR1
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CRAF_S259A_pcDNA3.1

    Plasmid

    #86505

    Purpose

    Expresses CRAF_S259A in mammalian cells

    Depositor

    Dustin Maly

    Article

    Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

    Insert

    CRAF (RAF1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S259A

    Promoter

    CMV

    Available since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1hLCAD_FLAG

    Plasmid

    #24491

    Depositor

    Eric Verdin

    Article

    Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

    Insert

    Long chain acyl-CoA dehydrogenase (ACADL Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 23, 2010

    Availability

    Academic Institutions and Nonprofits only

21. pcDNA3.1_hSMAD4

    Plasmid

    #212710

    Purpose

    The ORF of human SMAD4, variant 1 cDNA amplified from the RT human liver RNA was cloned at the NheI/HindIII sites of the pcDNA3.1(+), the mammalian expression vector.

    Depositor

    Catharine Ross, Reza Zolfaghari

    Insert

    Homo sapiens SMAD4 (SMAD4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3.1mLCAD_FLAG

    Plasmid

    #24484

    Depositor

    Eric Verdin

    Article

    Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

    Insert

    Long chain acyl-CoA dehydrogenase (Acadl Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 23, 2010

    Availability

    Academic Institutions and Nonprofits only

23. pcDNA3.1_27B3_hFc

    Plasmid

    #199618

    Purpose

    Encodes DARPin-hFc 27B3 for mammalian expression and column purification

    Depositor

    Shiva Tyagarajan

    Article

    Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

    Insert

    DARPin-hFc 27B3
    

    Use

    Tags

    hFc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.1_SNORD27h

    Plasmid

    #73066

    Purpose

    expression clone for human SNORD27 (C/D box snoRNA U27)

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

    Insert

    SNORD27 (SNORD27 Human)
    

    Use

    Tags

    no tag

    Expression

    Mammalian

    Mutation

    expression cassette for SNORD27

    Promoter

    CMV

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3.1dsLuc2CP

    Plasmid

    #68054

    Purpose

    expresses destabilized Firefly Luciferase

    Depositor

    Christopher Sullivan

    Article

    Seo et al J Virol. 2008 Oct;82(20):9823-8. doi: 10

    Insert

    Firefly luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

26. AlACR1_EYFP_pcDNA3.1

    Plasmid

    #161022

    Purpose

    Expresses strongly red-shifted AlACR1 in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al Proc Natl Acad Sci U S A. 2020 Sep 15;11

    Insert

    AlACR1
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. T1ACR1_EYFP_pcDNA3.1

    Plasmid

    #161027

    Purpose

    Expresses strongly red-shifted T1ACR1 in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al Proc Natl Acad Sci U S A. 2020 Sep 15;11

    Insert

    T1ACR1
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.1_SNORD27h_2ntmut

    Plasmid

    #73067

    Purpose

    expression clone for human mutated SNORD27 (C/D box snoRNA U27)

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

    Insert

    SNORD27 (E2F7 Human)
    

    Use

    Tags

    no tag

    Expression

    Mammalian

    Mutation

    2 nt mutation in AS box that binds E2F7 pre-mRNA

    Promoter

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

29. pcDNA3.1 PA-NL

    Plasmid

    #113445

    Purpose

    ProteinA-NanoLuc hybrid fusion control expression vector (for BRET and LuC normalization)

    Depositor

    Erich Wanker

    Article

    Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

    Insert

    ProteinA-NanoLuc
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. CRAF_S29A_pcDNA3.1

    Plasmid

    #86506

    Purpose

    Expresses CRAF_S29A in mammalian cells [Edit]

    Depositor

    Dustin Maly

    Article

    Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

    Insert

    CRAF (RAF1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S29A

    Promoter

    CMV

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA3.1_SNORD27h_115mut

    Plasmid

    #73068

    Purpose

    expression clone for human mutated SNORD27 (C/D box snoRNA U27)

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

    Insert

    SNORD27 (SNORD27 Human)
    

    Use

    Tags

    no tag

    Expression

    Mammalian

    Mutation

    expression cassette for SNORD27 with AS box from …

    Promoter

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

32. RaCCR1_EYFP_pcDNA3.1

    Plasmid

    #159122

    Purpose

    This plasmid encodes RaCCR1 rhodopsin domain

    Depositor

    John Spudich

    Article

    Sineshchekov et al mBio. 2020 Apr 21;11(2). pii: mBio.00657

    Insert

    RaCCR1
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA3.1mLCAD_K42R_FLAG

    Plasmid

    #24486

    Depositor

    Eric Verdin

    Article

    Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

    Insert

    Long chain acyl-CoA dehydrogenase (Acadl Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Lys42Arg

    Promoter

    Available since

    April 30, 2010

    Availability

    Academic Institutions and Nonprofits only

34. PgACR1_EYFP_pcDNA3.1

    Plasmid

    #161028

    Purpose

    Expresses PgACR1 in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al Proc Natl Acad Sci U S A. 2020 Sep 15;11

    Insert

    PgACR1
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA3.1_hE2F7mg_2ntmut

    Plasmid

    #73074

    Purpose

    human E2F7 minigene (exons 11,12,13/introns cassette) with mutated snord27 binding site

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

    Insert

    E2F7 (E2F7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    partial gene, exons 11,12,13 spaced by introns, 2…

    Promoter

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

36. pcDNA3.1_hE2F7mg_115mut

    Plasmid

    #73077

    Purpose

    human E2F7 minigene (exons 11,12,13/introns cassette) with inserted SNORD115 binding site

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

    Insert

    E2F7 (E2F7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    partial gene, exons 11,12,13 spaced by introns, m…

    Promoter

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3.1mLCAD_8KR_FLAG

    Plasmid

    #24485

    Depositor

    Eric Verdin

    Article

    Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

    Insert

    Long chain acyl-CoA dehydrogenase (Acadl Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Lys42, 156, 189, 240, 254, 318, 322, 358Arg (8KR)

    Promoter

    Available since

    April 30, 2010

    Availability

    Academic Institutions and Nonprofits only

38. AnsACR_mCherry_pcDNA3.1

    Plasmid

    #232598

    Purpose

    Expresses AnsACR_mCherry in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al eLife14:RP106508. doi: 10.7554/eLife.106

    Insert

    AnsACR
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. FtACR_mCherry_pcDNA3.1

    Plasmid

    #232599

    Purpose

    Expresses FtACR_mCherry in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al eLife14:RP106508. doi: 10.7554/eLife.106

    Insert

    FtACR
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. NlChR_mCherry_pcDNA3.1

    Plasmid

    #232600

    Purpose

    Expresses NlChR_mCherry in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al eLife14:RP106508. doi: 10.7554/eLife.106

    Insert

    NlChR
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pcDNA3.1alphaFEM1AalphaFLAG

    Plasmid

    #236460

    Purpose

    transient overexpression of FEM1A in mammalian cells

    Depositor

    Michele Pagano

    Article

    Dankert et al Cell Cycle. 2017 Mar 19;16(6):556-564. d

    Insert

    FEM1A (FEM1A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pcDNA3.1alphaFEM1BalphaFLAG

    Plasmid

    #236461

    Purpose

    transient overexpression of FEM1B in mammalian cells

    Depositor

    Michele Pagano

    Article

    Dankert et al Cell Cycle. 2017 Mar 19;16(6):556-564. d

    Insert

    FEM1B (FEM1B Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. mAMCase_CatD_6xHis_pcDNA3.1

    Plasmid

    #200229

    Purpose

    Expresses mouse Acidic Mammalian Chitinase catalytic domain with C-terminal 6xHis tag in pcDNA 3.1 vector

    Depositor

    James S Fraser

    Article

    Diaz et al Elife. 2024 Jun 17;12:RP89918. doi: 10.7

    Insert

    Acidic Mammalian Chitinase (Chia1 Mouse)
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    S354F

    Promoter

    CMV

    Available since

    Sept. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. HcCCR_mCherry_pcDNA3.1

    Plasmid

    #201687

    Purpose

    expresses HcCCR_mCherry fusion in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al mBio. 2022 Dec 20;13(6):e0303922. doi: 1

    Insert

    HcCCR
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. CovKCR1_mCherry_pcDNA3.1

    Plasmid

    #201688

    Purpose

    expresses CovKCR1_mCherry fusion in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al mBio. 2022 Dec 20;13(6):e0303922. doi: 1

    Insert

    CovKCR1
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pCDNA3.1_B1ChR2_TS_mScarlet_ER

    Plasmid

    #195191

    Purpose

    Highly K+ selective channelrhodopsin

    Depositor

    Peter Hegemann, Johannes Vierock

    Article

    Vierock et al Sci Adv. 2022 Nov 16:eadd7729.

    Insert

    Bilabrum sp. K+ selective channelrhodopsin 2
    

    Use

    Tags

    ER-export sequence, Kir2.1 trafficking motif, and…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pcDNA3.1_rSERT_C109A_Y107C

    Plasmid

    #190174

    Purpose

    For expression of a SERT mutant in mammalian cells and measuring accessibility in the SERT extracellular pathway

    Depositor

    Gary Rudnick

    Article

    Jacobs et al J Biol Chem. 2007 Oct 5;282(40):29441-7.

    Insert

    SLC6A4 Serotonin Transporter (Slc6a4 Rat)
    

    Use

    Tags

    FLAG, His6, and c-myc

    Expression

    Mammalian

    Mutation

    changed Cys109 to alanine, changed tyr107 to cyst…

    Promoter

    CMV

    Available since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pcDNA3.1_rSERT_X5C_S277C

    Plasmid

    #190177

    Purpose

    For mammalian cell expression of rat SERT and measurement of cytoplasmic pathway accessibility

    Depositor

    Gary Rudnick

    Article

    Zhang et al J Biol Chem. 2006 Nov 24;281(47):36213-2

    Insert

    rat serotonin transporter (Slc6a4 Rat)
    

    Use

    Tags

    FLAG, His6, and c-myc

    Expression

    Mammalian

    Mutation

    Cys15Ala, Cys21Ala, Cys109Ala, Ser277Cys, Cys357I…

    Promoter

    CMV

    Available since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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49. pcdna3.1_mouseST6galI

    Plasmid

    #178435

    Purpose

    murine St6gal1 expression plasmid

    Depositor

    Deborah Fass

    Article

    Ilani et al EMBO J. 2022 Oct 17:e111869. doi: 10.152

    Insert

    St6gal1 (St6gal1 Mouse)
    

    Use

    Tags

    6xHIS-TEV Cleavage Site

    Expression

    Mammalian

    Mutation

    Coding sequnce for amino acids 85-403

    Promoter

    CMV

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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50. mApple_pcDNA3.1+

    Plasmid

    #182855

    Purpose

    Monomer expression vector

    Depositor

    Salvatore Chiantia

    Article

    Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

    Insert

    mApple
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge