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pDB.His.MBP.3C NLRP1 CARD WT

Plasmid

#164008

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 CARD

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 (NLRP1 Human)

Use

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A FLAG-TEV-DPP9S LL100EE

Plasmid

#166798

Purpose

Mammalian cell expression vector for FLAG-tagged DPP9S. LL100EE mutant is loss of CARD8 and NLRP1 binding.

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Use

Tags

FLAG-TEV

Expression

Mammalian

Mutation

LL100EE

Promoter

CMV

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-mScarlet-MAVS-5'UTR

Plasmid

#184639

Purpose

Fluorescent reporter for mammalian cells: Expression of mScarlet with MAVS 5'UTR

Depositor

Hao Wu

Article

Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

mScarlet

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 2, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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PGL3Basic-Mdm2-T1

Plasmid

#32365

Depositor

Hong Wu

Article

Chang et al J Biol Chem. 2004 Jul 9;279(28):29841-8.

Insert

Mdm2 promoter (Mdm2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 6B CARD8 UPA-CARD-mCherry WT

Plasmid

#164011

Purpose

Mammalian expression vector encoding CARD8 UPA-CARD with a C-terminal mCherry tag for imaging

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3Basic-Mdm2-T2

Plasmid

#32366

Depositor

Hong Wu

Article

Chang et al J Biol Chem. 2004 Jul 9;279(28):29841-8.

Insert

Mdm2 promoter (Mdm2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
pET28a-DDX43-K292A

Plasmid

#134575

Purpose

Express human DDX43 mutant (K292A, ATPase dead) in E. coli

Depositor

Yuliang Wu

Article

Talwar et al J Biol Chem. 2017 Jun 23;292(25):10429-1

Insert

DDX43-K292A (DDX43 Human)

Use

Tags

His

Expression

Bacterial

Mutation

full-length (K292A)

Promoter

T7

Available since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 6A NLRP1 CARD-FLAG WT

Plasmid

#163978

Purpose

Mammalian expression vector encoding human NLRP1 CARD with a C-terminal FLAG tag

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 (NLRP1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pET29a-hNABP1-C

Plasmid

#128311

Purpose

Express hNABP1 C-terminus (125–204 aa) in E. coli with a C-terminal His-tag

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

Insert

Human nucleic acid-binding protein 1 (hNABP1)-C terminus (125-204 aa) (NABP1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pGL3Basic-Mdm-P1

Plasmid

#32367

Depositor

Hong Wu

Article

Chang et al J Biol Chem. 2004 Jul 9;279(28):29841-8.

Insert

Mdm2 promoter (Mdm2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
pUASTP2

Plasmid

#13843

Depositor

Ting Wu

Article

Bateman et al Genetics. 2006 Jun . 173(2):769-77.

Insert

mini-white (w Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 LIC 6A NLRP1-FLAG E1322R

Plasmid

#166809

Purpose

Mammalian cell expression vector for FLAG-tagged NLRP1. The E1322R mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

E1322R

Promoter

CMV

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-SARS-CoV-2 SL1 alone-Luciferase

Plasmid

#191482

Purpose

Luciferase reporter for SARS-CoV-2 5' UTR SL1 alone

Depositor

Hao Wu

Article

Shi et al bioRxiv. 2020 Sep 18. doi: 10.1101/2020.

Insert

SARS-CoV-2 5' UTR SL1 alone

Use

Lentiviral and Luciferase

Tags

Expression

Mutation

Contains only the stem-loop1 region of SARS-CoV-2…

Promoter

Available since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pUASTP2.1

Plasmid

#13842

Depositor

Ting Wu

Article

Bateman et al Genetics. 2006 Jun . 173(2):769-77.

Insert

mini-white (w Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only

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pFastBac-HS-NLRC4Δcard

Plasmid

#177276

Purpose

Purification of mouse NLRC4 (90-1024) from insect cells

Depositor

Hao Wu

Article

Zhang et al Science. 2015 Oct 23;350(6259):404-9. do

Insert

NLRC4 (Nlrc4 Mouse)

Use

Tags

HIS-SUMO-PreScission

Expression

Insect

Mutation

I586T, N761S, M885V, K915R and F972L- please see …

Promoter

polyhedrin

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-INTS3-N

Plasmid

#128416

Purpose

Express INTS3 N-termini (1-513 aa) in E. coli with a GST tag (N-terminal)

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

Insert

INTS3 (INTS3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

N-terminal region (1–513 aa)

Promoter

tac promoter

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-INTS3-C

Plasmid

#128417

Purpose

Express INTS3 C-termini (514-1042 aa) in E. coli with a GST tag (N-terminal)

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

Insert

INTS3 (INTS3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

INTS3 C-terminal region (514-1042 aa)

Promoter

Tac promoter

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pDB-His-MBP-SARM1

Plasmid

#195762

Purpose

Purification of SARM1 from E.coli

Depositor

Hao Wu

Insert

SARM1 (SARM1 Human)

Use

Tags

HIS-MBP-TEV

Expression

Bacterial

Mutation

truncation of first 19 residues

Promoter

T7

Available since

Feb. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A FLAG-TEV-DPP9S S730A

Plasmid

#166801

Purpose

Mammalian cell expression vector for FLAG-tagged DPP9S. The S730A mutant is catalytically dead.

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Use

Tags

FLAG-TEV

Expression

Mammalian

Mutation

S730A

Promoter

CMV

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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TRBV12-4

Plasmid

#123446

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRBV12-4 (TRBV12-4 Human)

Use

Tags

Expression

Bacterial

Mutation

WT

Promoter

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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