We narrowed to 13,473 results for: crispr cas9

1. pLPhygCAS9

   Plasmid

   #63555

   Purpose

   Expresses CAS9 in Leishmania

   Depositor

   Greg Matlashewski

   Article

   Zhang et al MBio. 2015 Jul 21;6(4). pii: e00861-15.

   Insert

   Humanized Streptococcus pyogenes Cas9 from PX330 (Addgene)
   

   Use

   Leishmania expression

   Tags

   flag

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. BPK4410 - human expression plasmid for SpCas9 Cluster 1 (HypaCas9)

   Plasmid

   #101178

   Purpose

   Human expression plasmid for SpCas9 Cluster 1 variant (HypaCas9): CMV-T7-hSpCas9-Cluster1(N692A, M694A, Q695A, H698A)-NLS(SV40)-3xFLAG

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   hSpCas9-Cluster1(N692A/M694A/Q695A/H698A)
   

   Use

   Tags

   3x FLAG and NLS (SV40)

   Expression

   Mammalian

   Mutation

   N692A/M694A/Q695A/H698A

   Promoter

   CMV

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGW_RUBY_EggCas9

   Plasmid

   #225990

   Purpose

   To clone entry vector with CRISPR guide RNA via Gateway cloning; it has an Egg cell-specific expression of Cas9 and the plant is selected by the red color due to constitutive expression of RUBY

   Depositor

   Imran Siddiqi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Oct. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. XClone_Cas9_KRAB_T2A_BFP

   Plasmid

   #141208

   Purpose

   Tunable and temporal expression control of dCas9

   Depositor

   Rory Johnson

   Insert

   dCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEM-eCas9-D1135E

   Plasmid

   #154926

   Purpose

   aTc inducible eCas9 with D1135E mutation

   Depositor

   Michael Lynch

   Article

   Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

   Insert

   eCas9-D1135E
   

   Use

   CRISPR and Synthetic Biology

   Tags

   ssrA

   Expression

   Bacterial

   Mutation

   K848A, K1003A, R1060A, D1135E

   Promoter

   pTet

   Available Since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEM-OptiCas9-D1135E

   Plasmid

   #154928

   Purpose

   aTc inducible OptiCas9 with D1135E mutation

   Depositor

   Michael Lynch

   Article

   Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

   Insert

   OptiCas9-D1135E
   

   Use

   CRISPR and Synthetic Biology

   Tags

   ssrA

   Expression

   Bacterial

   Mutation

   R661A, K1003H, D1135E

   Promoter

   pTet

   Available Since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEM-HypaCas9-D1135E

   Plasmid

   #154930

   Purpose

   aTc inducible HypaCas9 with D1135E mutation

   Depositor

   Michael Lynch

   Article

   Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

   Insert

   HypaCas9-D1135E
   

   Use

   CRISPR and Synthetic Biology

   Tags

   ssrA

   Expression

   Bacterial

   Mutation

   N692A/M694A/Q695A/H698A/D1135E

   Promoter

   pTet

   Available Since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEM-Cas9-D1135E

   Plasmid

   #154931

   Purpose

   aTc inducible Cas9 with D1135E mutation

   Depositor

   Michael Lynch

   Article

   Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

   Insert

   Cas9-D1135E
   

   Use

   CRISPR and Synthetic Biology

   Tags

   ssrA

   Expression

   Bacterial

   Mutation

   D1135E

   Promoter

   pTet

   Available Since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Cas9-sgRNA-A

   Plasmid

   #149369

   Purpose

   Expresses SpCas9 and sgRNA targeting the lenti-CDDR reporter

   Depositor

   Tarek Abbas

   Article

   Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592

   Inserts

   Cas9
   sgRNA-A
   Puromycin resistance
   

   Use

   CRISPR

   Tags

   3xFLAG, Nucleoplasmin NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CBh, PGK, and U6

   Available Since

   Nov. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJSC173 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9) variant

    Plasmid

    #101218

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9) variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant N692A/M694A/Q695A/H698A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    N692A, M694A, Q695A and H698A

    Promoter

    T7

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p15A_J23108_SpydCas9_CmR

    Plasmid

    #119163

    Purpose

    Expresses S. pyogenes dCas9

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    dCas9 from S. pyogenes
    

    Use

    Crispr

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCas9cur

    Plasmid

    #71538

    Purpose

    Constitutive expression of Cas9 gene and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.

    Depositor

    Tao Chen

    Article

    Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10

    Inserts

    Cas9
    Plasmid curing system
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available Since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pX459V2.0-HypaCas9

    Plasmid

    #108294

    Purpose

    pX459 V2.0 (Plasmid #62988) with the N692A, M694A, Q695A, and H698A mutations

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pX330-HypaCas9

    Plasmid

    #108302

    Purpose

    pX330 (Plasmid #42230) with the N692A, M694A, Q695A, and H698A mutations

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Cas9 NGFR

    Plasmid

    #155279

    Purpose

    Lentiviral Cas9 expression with NGFR marker

    Depositor

    Martin Carroll

    Insert

    spCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Cas9 CFP

    Plasmid

    #155278

    Purpose

    Lentiviral Cas9 expression with CFP marker

    Depositor

    Martin Carroll

    Insert

    spCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEM-Cas9HF1-D1135E

    Plasmid

    #154924

    Purpose

    aTc inducible Cas9HF1 with D1135E mutation

    Depositor

    Michael Lynch

    Article

    Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

    Insert

    Cas9HF1-D1135E
    

    Use

    CRISPR and Synthetic Biology

    Tags

    ssrA

    Expression

    Bacterial

    Mutation

    N497A/R661A/Q695A/Q926A/D1135E and stop codon on …

    Promoter

    pTet

    Available Since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHS26_10xHis_MBP_TEV_VRER-Cas9

    Plasmid

    #244829

    Purpose

    Bacterial expression of VRER Cas9 for protein purification

    Depositor

    Jennifer Doudna

    Article

    Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

    Insert

    VRER Cas9
    

    Use

    CRISPR

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Mutation

    D1135V/G1218R/R1335E/T1337R

    Promoter

    T7

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHS16_10xHis_MBP_TEV_xPBA-Cas9

    Plasmid

    #244830

    Purpose

    Bacterial expression of xPBA Cas9 for protein purification

    Depositor

    Jennifer Doudna

    Article

    Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

    Insert

    xPBA Cas9
    

    Use

    CRISPR

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHS25_10xHis_MBP_TEV_EQR-Cas9

    Plasmid

    #244828

    Purpose

    Bacterial expression of EQR Cas9 for protein purification

    Depositor

    Jennifer Doudna

    Article

    Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

    Insert

    EQR Cas9
    

    Use

    CRISPR

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Mutation

    D1135E/R1335Q/T1337R

    Promoter

    T7

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge