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pRSETB/mStayGold

Plasmid

#212017

Purpose

mStayGold has an n1 adaptor at its N-terminus and is useful for C-terminal tagging. This construct is used also for expression of mStayGold itself.

Depositor

Atsushi Miyawaki

Publication

Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Type

Empty backbone

Tags

6xHis, T7 tag, Xpress tag, mStayGold

Expression

Bacterial

Promoter

T7

Available since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pSicoR-Ef1a-mCh-Puro

Plasmid

#31845

Purpose

Cre-regulated lentiviral shRNA vector. Cre addition causes both mCherry and shRNA to be recombined out of the construct, turning OFF shRNA expression. Includes puromycin selection.

Depositor

Bruce Conklin

Publication

Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

Type

Empty backbone

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

U6 for shRNA; Ef1alpha for mCherry-puromycin

Available since

Jan. 3, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

19 Articles

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Human ICAM-1

Plasmid

#8632

Depositor

Timothy Springer

Publication

Staunton et al Cell 1988 Mar 25;52(6):925-33.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ICAM-1 (ICAM1 Human)

Expression

Mammalian

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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BFP-Sec61 beta

Plasmid

#49154

Purpose

expression of Sec61 beta fused to TagBFP, used as a general ER marker

Depositor

Gia Voeltz

Publication

Zurek et al Traffic. 2011 Jan;12(1):28-41. doi: 10.1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Sec61 beta (SEC61B Human)

Tags

TagBFP

Expression

Mammalian

Promoter

CMV

Available since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pAAV-cFos-tTA-pA

Plasmid

#66794

Purpose

Expresses TetActivator protein under the control of the c-fos promoter for use in TetTaging

Depositor

William Wisden

Publication

Zhang et al Nat Neurosci. 2015 Apr;18(4):553-61. doi

Insert

cfos promoter linked to the tet activator gene

Use

AAV

Tags

no

Expression

Mammalian

Available since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pEZT-BM

Plasmid

#74099

Purpose

Multi-purpose rapid titer bacmam expression vector

Depositor

Ryan Hibbs

Publication

Morales-Perez et al Structure. 2016 Mar 24. pii: S0969-2126(

Type

Empty backbone

Use

Bacmam, baculovirus

Tags

EGFP following p10 promoter

Expression

Mammalian

Promoter

CMV, p10

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

31 Articles

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pMSP1E1

Plasmid

#20062

Depositor

Stephen Sligar

Publication

Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

Insert

MSP1E1 (APOA1 synthetic gene)

Tags

6-His

Expression

Bacterial

Mutation

"extended" MSP1; contains repeat of hel…

Available since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pBAC-BA-lacZ

Plasmid

#13423

Depositor

Keith Joung

Publication

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

BA

Use

Cre/Lox

Expression

Bacterial

Available since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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Nanog5P reporter

Plasmid

#16337

Depositor

Austin Cooney

Publication

Gu et al Mol Cell Biol. 2005 Oct . 25(19):8507-19

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Nanog promoter (Nanog Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Promoter

Mouse Nanog promoter

Available since

Dec. 6, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pST1374

Plasmid

#13426

Depositor

Keith Joung

Publication

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Type

Empty backbone

Tags

Flag

Expression

Mammalian

Available since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pCMV5B-Flag-Smurf1 wt

Plasmid

#11752

Depositor

Jeff Wrana

Publication

Zhu et al Nature. 1999 Aug 12. 400(6745):687-93.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SMURF1 (SMURF1 Human)

Tags

Flag

Expression

Mammalian

Available since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

7 Articles

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pEG BacMam

Plasmid

#160451

Purpose

vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

Depositor

Eric Gouaux

Publication

Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pHaloTag-EGFP

Plasmid

#86629

Purpose

Expresses HaloTag-eGFP tandem (cytosolic)

Depositor

Thomas Leonard, Ivan Yudushkin

Publication

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HaloTag

Expression

Mammalian

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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mEGFP-NbALFA

Plasmid

#159986

Purpose

For the expresson and purification of the EGFP-tagged anti-ALFA tag nanobody (NbALFA)

Depositor

Motoyuki Hattori

Publication

Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

Insert

mEGFP-NbALFA

Tags

8histidine-HRV3C protease cleavage site

Expression

Bacterial

Mutation

3GS linker between mEGFP and NbALFA

Available since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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LV-CFP

Plasmid

#25998

Depositor

Elaine Fuchs

Publication

Beronja et al Nat Med. 2010 Jul . 16(7):821-7.

Insert

H2B-CFP (H2BC21 Human, A. victoria)

Use

Lentiviral

Expression

Mammalian

Available since

Dec. 10, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pMXs-gw

Plasmid

#18656

Depositor

Shinya Yamanaka

Publication

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

Gateway cassette A

Use

Retroviral

Expression

Mammalian

Available since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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GFP-MAPPER

Plasmid

#117721

Purpose

MAPPER Selectively Labels ER-PM Junctions

Depositor

Jen Liou

Publication

Chang et al Cell Rep. 2013 Nov 14;5(3):813-25. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MAPPER

Tags

GFP

Expression

Mammalian

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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Cav1-GFP

Plasmid

#14433

Depositor

Ari Helenius

Publication

Tagawa et al J Cell Biol. 2005 Aug 29. 170(5):769-79.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Caveolin-1 (CAV1 Canis familalis)

Tags

EGFP

Expression

Mammalian

Available since

March 23, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pEGFP VAMP3

Plasmid

#42310

Depositor

Thierry Galli

Publication

Galli et al Mol Biol Cell. 1998 Jun;9(6):1437-48.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

VAMP 3 (VAMP3 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 28, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pMSCV-OPA1

Plasmid

#26047

Depositor

David Chan

Publication

Song et al J Cell Biol. 2007 Aug 27;178(5):749-55.

Insert

OPA1 (OPA1 Human)

Use

Retroviral

Expression

Mammalian

Available since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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