We narrowed to 17,082 results for: emb

1. pEntry_SARS-CoV-1-Membrane

   Plasmid

   #168840

   Purpose

   Gateway-compatible Entry vector

   Depositor

   Pascal Falter-Braun

   Article

   Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

   Insert

   SARS-CoV-1-Membrane (M )
   

   Use

   Gateway-compatible entry vector

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. membrane-mCherry-SpVasa-C3

   Plasmid

   #185587

   Purpose

   to recruit Vasa-C3 to membrane

   Depositor

   Mamiko Yajima

   Article

   Fernandez-Nicolas et al Dev Dyn. 2019 Dec;248(12):1273-1285. doi

   Insert

   Vasa-C3
   

   Use

   In vitro transcription (mrna synthesis)

   Tags

   mCherry and membrane targeting sequence from Neur…

   Mutation

   C3 mutant

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. membrane-mCherry-SpVasa

   Plasmid

   #185586

   Purpose

   to recruit Vasa to membrane

   Depositor

   Mamiko Yajima

   Article

   Fernandez-Nicolas et al Dev Dyn. 2019 Dec;248(12):1273-1285. doi

   Insert

   Vasa
   

   Use

   In vitro transcription (mrna synthesis)

   Tags

   mCherry, S-Tag and membrane targeting sequence fr…

   Available Since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. membrane-HsPLK1-dead

   Plasmid

   #185572

   Purpose

   to see protein dynamics

   Depositor

   Mamiko Yajima

   Article

   Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

   Insert

   PLK1-dead (PLK1 Human)
   

   Use

   In vitro transcription (mrna synthesis)

   Tags

   membrane targeting sequence from Neuromodulin (ML…

   Mutation

   K82R kinase-dead mutant

   Available Since

   July 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Plasma membrane-targeted optoPLD

   Plasmid

   #140114

   Purpose

   Encodes CRY2-mCherry-PLD-P2A-CIBN-CAAX for mammalian expression

   Depositor

   Jeremy Baskin

   Article

   Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   Phospholipase D
   

   Tags

   CAAX, CIBN, CRY2, P2A, and mCherry

   Expression

   Mammalian

   Available Since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Plasma membrane-targeted optodPLD

   Plasmid

   #140061

   Purpose

   Encodes CRY2-mCherry-dPLD-P2A-CIBN-CAAX for mammalian expression

   Depositor

   Jeremy Baskin

   Article

   Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   Phospholipase D
   

   Tags

   CAAX, CIBN, CRY2, P2A, and mCherry

   Expression

   Mammalian

   Mutation

   H170A (catalytic dead)

   Available Since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSP64-membrane-mCherry-LOV

   Plasmid

   #135599

   Purpose

   Expression of membrane-mCherry-LOV mRNA for microinjection for use of optogenetics in S. purpuratus

   Depositor

   Mamiko Yajima

   Article

   Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

   Insert

   membrane-mCherry-LOV
   

   Use

   In vitro transcription (mrna synthesis)

   Tags

   LOV and mCherry

   Promoter

   Unknown

   Available Since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. cASIC1 concatemer - fully assembled

   Plasmid

   #233133

   Purpose

   To create cASIC1 channels of defined stoichiometry in mammalian cells

   Depositor

   David MacLean

   Article

   Purohit et al Biophys J. 2024 Oct 15;123(20):3507-3518

   Insert

   ASIC1 (ASIC1 Chicken)
   

   Expression

   Mammalian

   Available Since

   April 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PhOTO-N (pMTB:memb-Cerulean-2A-H2B-Dendra2)

   Plasmid

   #92400

   Purpose

   Constitutive expression of nuclear green-to-red photoconvertible Dendra2 and the membrane blue fluorescent protein Cerulean.

   Depositor

   Periklis Pantazis

   Article

   Dempsey et al PLoS One. 2012;7(3):e32888. doi: 10.1371

   Inserts

   Dendra2
   Cerulean
   

   Use

   Zebrafish expression

   Tags

   H2B tag and membrane tag

   Available Since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pPW3756 : CMVd3-ERmembrane-HaloTag-KKMP

    Plasmid

    #185678

    Purpose

    Low-level transient expression of a single HaloTag protein with a C-terminal KKMP ER retention signal, targeted to the ER membrane.

    Depositor

    Peter Walter

    Article

    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

    Insert

    ERmembrane-HaloTag-KKMP
    

    Expression

    Mammalian

    Available Since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PhOTO-M (pMTB:memb-Dendra2-2A-H2B-Cerulean)

    Plasmid

    #92401

    Purpose

    Constitutive expression of membrane green-to-red photoconvertible Dendra2 and the nuclear blue fluorescent protein Cerulean.

    Depositor

    Periklis Pantazis

    Article

    Dempsey et al PLoS One. 2012;7(3):e32888. doi: 10.1371

    Inserts

    Dendra2
    Cerulean
    

    Use

    Zebrafish expression

    Tags

    H2B tag and membrane tag

    Promoter

    bactin2

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Nano-lantern-plasma membrane/pcDNA3

    Plasmid

    #51975

    Purpose

    mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, targeted to the plasma membrane

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern
    

    Tags

    HIS and Lyn

    Expression

    Mammalian

    Mutation

    S257G in Rluc8

    Available Since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pPW3757 : CMVd3-ERmembrane-2xHaloTag-KKMP

    Plasmid

    #185679

    Purpose

    Low-level transient expression of two tandem HaloTag proteins targeted to the ER membrane.

    Depositor

    Peter Walter

    Article

    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

    Insert

    ERmembrane-2xHaloTag-KKMP
    

    Expression

    Mammalian

    Available Since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. HA-MAO-N Mitochondrial Membrane-Targeting Cloning Vector

    Plasmid

    #139015

    Purpose

    To express a protein of interest fused to the N-terminus of HA-MAO for mitochondrial outer membrane targeting

    Depositor

    Ken-Ichi Takemaru

    Type

    Empty backbone

    Tags

    HA-MAO

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPW3781 : CMVd3-ERmembrane-GST-HaloTag-KKMP

    Plasmid

    #185686

    Purpose

    Low-level transient expression of a HaloTag protein targeted to the ER membrane, fused with a GST for constitutive dimerization.

    Depositor

    Peter Walter

    Article

    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

    Insert

    ERmembrane-GST-HaloTag-KKMP
    

    Expression

    Mammalian

    Available Since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLVX-EF1a-EGFP-Membrin-IRES-Puromycin

    Plasmid

    #134865

    Purpose

    Expresses EGFP-tagged Golgi apparatus marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    Membrin (GOSR2 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMOS010: CheRiff-eGFP channelrhodopsin (plasma membrane)

    Plasmid

    #163044

    Purpose

    CheRiff-eGFP channelrhodopsin (plasma membrane)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    CheRiff
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPW3783 : CMVd3-ERmembrane-GST-2xHaloTag-KKMP

    Plasmid

    #185687

    Purpose

    Low-level transient expression of two tandem HaloTag proteins targeted to the ER membrane, fused with a GST for constitutive dimerization (making a HaloTag tetramer).

    Depositor

    Peter Walter

    Article

    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

    Insert

    ERmembrane-GST-2xHaloTag-KKMP
    

    Expression

    Mammalian

    Available Since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRK5 FLAG membrane-bound lipin-1 (TMD)

    Plasmid

    #120277

    Purpose

    Expression of ER/Golgi-tethered lipin-1 in mammalian cells

    Depositor

    Sirio Dupont

    Article

    Romani et al Nat Cell Biol. 2019 Mar;21(3):338-347. d

    Insert

    Lipin-1 (Lpin1 Mouse)
    

    Tags

    FLAG and in-frame fusion with the TMD Golgi-local…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pD649-HAsp-EMB-COMP5AP-AviTag-9xHis

    Plasmid

    #157244

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    EMB (EMB Human)
    

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge