We narrowed to 1,249 results for: vent

1. pCTCON2-GFP-TAG

   Plasmid

   #158131

   Purpose

   Single fluorescent protein reporter (GFP) for ncAA incorporation (TAG construct)

   Depositor

   James Van Deventer

   Article

   Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Introduced a stop codon in place of the tyrosine …

   Promoter

   GAL1-10

   Available since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDN43

   Plasmid

   #61346

   Purpose

   mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS11/PKIt NES)

   Depositor

   Barbara Di Ventura, Roland Eils

   Article

   Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

   Insert

   mCherry
   

   Use

   Tags

   GS linker-AsLov2-biNLS11 and PKIt NES

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKS-DTA miR-106b~25

   Plasmid

   #19030

   Depositor

   Tyler Jacks

   Article

   Ventura et al Cell. 2008 Mar 7. 132(5):875-86.

   Insert

   microRNA 106a-25 (Mir25 Mouse)
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBS-miR106a-363

   Plasmid

   #19028

   Depositor

   Tyler Jacks

   Article

   Ventura et al Cell. 2008 Mar 7. 132(5):875-86.

   Insert

   microRNA 106a-363 (Mir106a Mouse)
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 25, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCTCON2-BXG-altTAG

   Plasmid

   #158132

   Purpose

   Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

   Depositor

   James Van Deventer

   Article

   Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

   Insert

   BFP-GFP fragment
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Cloned in a TAG codon at the first serine residue…

   Promoter

   GAL1-10

   Available since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCTCON2-BXG-altTAG2

   Plasmid

   #158134

   Purpose

   Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

   Depositor

   James Van Deventer

   Article

   Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

   Insert

   BFP-GFP fragment
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Cloned in a TAG codon at the second serine residu…

   Promoter

   GAL1-10

   Available since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCTCON2-BXG-altTAG3

   Plasmid

   #158136

   Purpose

   Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

   Depositor

   James Van Deventer

   Article

   Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

   Insert

   BFP-GFP fragment
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Cloned in a TAG codon at the third alanine residu…

   Promoter

   GAL1-10

   Available since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCTCON2-BXG-altTAG4

   Plasmid

   #158137

   Purpose

   Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

   Depositor

   James Van Deventer

   Article

   Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

   Insert

   BFP-GFP fragment
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Cloned in a TAG codon at the third serine residue…

   Promoter

   GAL1-10

   Available since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pX333

   Plasmid

   #64073

   Purpose

   Vector for tandem expression of two sgRNAs from two independent U6 promoters. Cas9 is expressed by Cbh promoter.

   Depositor

   Andrea Ventura

   Article

   Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   3XFLAG-Cas9

   Expression

   Mammalian

   Mutation

   Promoter

   CBh; U6

   Available since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. trLATmEos3.2

    Plasmid

    #203745

    Purpose

    Encodes the transmembrane domain from human LAT with mEos3.2 fused on the C terminus. Used as a fluorescent plasma membrane probe.

    Depositor

    Sarah Veatch

    Article

    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

    Insert

    trLAT (LAT Human)
    

    Use

    Tags

    mEos3.2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. Adeno EA

    Plasmid

    #64071

    Purpose

    Adenovirus for the expression of gRNAs targeting intron 19 of murine Alk and intron 14 of Eml4

    Depositor

    Andrea Ventura

    Article

    Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

    Insert

    U6_sgRNA(Alk)_U6_sgRNA(Eml4)_CBh_FLAG-Cas9
    

    Use

    Adenoviral

    Tags

    FLAG-Cas9

    Expression

    Mutation

    Promoter

    U6 and CBh

    Available since

    July 23, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pBLADE(FP6**)-mCherry

    Plasmid

    #168049

    Purpose

    Expresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter. mCherry expression is controlled by the PBAD promoter.

    Depositor

    Barbara Di Ventura

    Article

    Romano et al Nat Chem Biol. 2021 Apr 26. pii: 10.1038

    Inserts

    Blue light-inducible AraC dimers in Escherichia coli
    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23101** and PBAD

    Available since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMGIB-3xFLAG-dyskerin

    Plasmid

    #53607

    Purpose

    Retroviral expression of human dyskerin

    Depositor

    Steven Artandi

    Article

    Venteicher et al Science. 2009 Jan 30;323(5914):644-8. do

    Insert

    dyskerin (DKC1 Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pBLADE(FP6*)-mCherry

    Plasmid

    #168048

    Purpose

    Expresses the synthetic gene BLADE FP6 under the constitutive J23101* promoter. mCherry expression is controlled by the PBAD promoter.

    Depositor

    Barbara Di Ventura

    Article

    Romano et al Nat Chem Biol. 2021 Apr 26. pii: 10.1038

    Inserts

    Blue light-inducible AraC dimers in Escherichia coli
    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23101* and PBAD

    Available since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMGIB-3xFLAG-pontin

    Plasmid

    #53608

    Purpose

    Retroviral expression of human pontin

    Depositor

    Steven Artandi

    Article

    Venteicher et al Cell. 2008 Mar 21;132(6):945-57. doi: 10

    Insert

    pontin (RUVBL1 Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    pontin CDS mutated to confer resistance to shRNA.…

    Promoter

    LTR

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pCTCON2-BXG-2TAG

    Plasmid

    #158138

    Purpose

    Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (2 TAG construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    BFP-GFP fragment
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Cloned in a TAG codon at the first serine residue…

    Promoter

    GAL1-10

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCIRCgp41-1

    Plasmid

    #74227

    Purpose

    Vector for SICLOPPS-mediated protein circularization using the gp41-1 intein.

    Depositor

    Barbara Di Ventura, Roland Eils

    Article

    Waldhauer et al Mol Biosyst. 2015 Nov 10;11(12):3231-43.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    gp41-1 C-intein and gp41-1 N-intein

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lac

    Available since

    April 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. TFORF3502

    Plasmid

    #144978

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    VENTX (VENTX Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCIRCNpu

    Plasmid

    #74226

    Purpose

    Vector for SICLOPPS-mediated protein circularization using the Nostoc punctiforme DnaE intein.

    Depositor

    Barbara Di Ventura, Roland Eils

    Article

    Waldhauer et al Mol Biosyst. 2015 Nov 10;11(12):3231-43.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Npu DnaE C-intein and Npu DnaE N-intein

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lac

    Available since

    April 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. gRNA-10Xcapture-PuroR

    Plasmid

    #211496

    Purpose

    Plasmid for the expression of SAM-compatible gRNA for CRISPRa with capturer sequence for 10X Genomics sequencing

    Depositor

    Antonella Fidanza, Lesley Forrester

    Article

    Petazzi et al Elife. 2024 Dec 23;13:RP94884. doi: 10.7

    Inserts

    U6-gRNA
    Ef1a-PuroR
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a and U6

    Available since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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