We narrowed to 7,580 results for: Ski;

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pNL4-3 envFS eGFP gag2xFS

Plasmid

#101341

Purpose

1st frameshift is CG nucleotide insertion in MA at nt 832. 2nd is a CTAG addition in CA at nt 1508.

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pUAS-Cre

Plasmid

#50797

Purpose

Expression of Cre recombinase under the control of the UAS-promoter

Depositor

Connie Cepko

Article

Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

Insert

Cre recombinase

Use

Multi-species

Promoter

UAS-hsp70

Available Since

March 20, 2014

Availability

Academic Institutions and Nonprofits only

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pMJ824

Plasmid

#39314

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

GST and His6

Expression

Bacterial

Promoter

T7

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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mEGFP-biRhoBAST

Plasmid

#196348

Purpose

Expression of mEGFP mRNA with biRhoBAST in 3'UTR

Depositor

Murat Sunbul

Article

Buhler et al Nat Chem Biol. 2023 Apr;19(4):478-487. d

Insert

mEGFP-biRhoBAST

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pRS413-CUP1pr

Plasmid

#221109

Purpose

Copper-inducible gene expression

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Expression

Yeast

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pBAD-luxKOk

Plasmid

#229944

Purpose

Bacterial expression of a de novo designed, FRET-based neoLux1.2-mKOk fusion bioluminescent probe (max. Em=562nm)

Depositor

Andy Yeh

Article

Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

Insert

luxKOk

Tags

His-tag

Expression

Bacterial

Promoter

araBAD

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pBAD-luxGold

Plasmid

#229943

Purpose

Bacterial expression of a de novo designed, FRET-based neoLux1.2-mGold fusion bioluminescent probe (max. Em=530nm)

Depositor

Andy Yeh

Article

Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

Insert

luxGold

Tags

His-tag

Expression

Bacterial

Promoter

araBAD

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-Ace2_D92N_E199V-mNeon

Plasmid

#129256

Purpose

Express Ace2_D92N_E199V-mNeon in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Ace2_D92N_E199V-mNeon

Expression

Mammalian

Mutation

D92N, E199V

Promoter

CAG

Available Since

Oct. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 Stu2 1-306

Plasmid

#38315

Depositor

Anthony Hyman

Article

Widlund et al Mol Biol Cell. 2012 Sep 19.

Insert

5' 918 bp from STU2 ORF (STU2 Budding Yeast)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pGL3-LRF

Plasmid

#66936

Purpose

Destination vector; gateway cassette cloned upstream of basal promoter driving luciferase. Forward orientation (attR1, attR2).

Depositor

Alvaro Monteiro

Article

Buckley et al Nat Protoc. 2016 Jan;11(1):46-60. doi: 1

Type

Empty backbone

Use

Luciferase

Expression

Mammalian

Promoter

SV-40

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only
pMito.iGABASnFR.F102Y.Y137L

Plasmid

#112177

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR.F102Y.Y137L

Expression

Mammalian

Mutation

F102Y.Y137L

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_mitoNanobody

Plasmid

#136622

Purpose

Plasmid encoding outer mitochondrial localization of the Enhancer nanobody for GFP as a SNAP-Tag-fusion

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

NTOM20-SNAP-Tag-EnhancerNb

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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EGFP-Septin 11 isoform II

Plasmid

#120317

Purpose

Septin 11 isoform II mammalian expression (EGFP-tag at the N-terminus)

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 (Sept11 Rat)

Tags

EGFP

Expression

Mammalian

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-Positron-ST

Plasmid

#129254

Purpose

Express soma-localized Positron in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Positron-ST

Expression

Mammalian

Mutation

D92N, E199V

Promoter

CAG

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pHPyV7-713a

Plasmid

#24728

Depositor

Christopher Buck

Article

Schowalter et al Cell Host Microbe. 2010 Jun 25;7(6):509-

Insert

Full genome of Human polyomavirus 7

Use

Viral clone

Available Since

June 8, 2010

Availability

Academic Institutions and Nonprofits only

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pBW842_pCAG-pExpr-FP-DECODER

Plasmid

#87551

Purpose

Expresses BFP, GFP, iRFP, and mRuby in response to combinations of Cre and Flp recombinases

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

mtagBFP, EGFP, iRFP720, mRuby2

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pHPyV6-607a

Plasmid

#24727

Depositor

Christopher Buck

Article

Schowalter et al Cell Host Microbe. 2010 Jun 25;7(6):509-

Insert

Full genome of Human polyomavirus 6

Use

Viral clone

Available Since

June 8, 2010

Availability

Academic Institutions and Nonprofits only

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pKPY514

Plasmid

#62598

Purpose

Encodes Thr251Gly-EcPheRS Under IPTG-Inducible (PT5) Control

Depositor

David Tirrell

Article

Yuet et al Proc Natl Acad Sci U S A. 2015 Feb 17. p

Insert

Thr251Gly-EcPheRS

Tags

6xHis

Expression

Bacterial

Mutation

Thr251Gly

Promoter

T5

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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Tol2-krt4-RFP

Plasmid

#97149

Purpose

As a control of miRNA sponge

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Type

Empty backbone

Use

Zebrafish

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pUASp YFP Rabx6 WT

Plasmid

#53479

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rabx6 (RabX6 Fly)

Tags

YFP

Expression

Insect

Promoter

GAL4

Available Since

May 12, 2014

Availability

Academic Institutions and Nonprofits only
pMito.iGABASnFR.F102G

Plasmid

#112176

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR.F102G

Expression

Mammalian

Mutation

F102G

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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Myo 1b-myc

Plasmid

#135064

Purpose

Expression of rat Myo 1b (Myr 1) in mammalian cells. C-terminal myc-tag.

Depositor

Martin Bähler

Article

Ruppert et al J Cell Biol. 1993 Mar;120(6):1393-403. d

Insert

Myo 1b with 5' UTR (Myo1b Rat)

Tags

Linker and Myc

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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SEPT5 WT

Plasmid

#27272

Depositor

Harish Pant

Article

Amin et al J Neurosci. 2008 Apr 2. 28(14):3631-43.

Insert

Septin 5 (SEPTIN5 Human)

Tags

Myc (MASMQKLISEEDL)

Expression

Mammalian

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pCDNA3.1-Muc2D1D2D3cysD1

Plasmid

#155214

Purpose

Expresses human MUC2 residues 21-1397

Depositor

Deborah Fass

Article

Javitt et al Cell. 2020 Oct 29;183(3):717-729.e16. do

Insert

Muc2 D1D2D3cysD1 (MUC2 Human)

Tags

6xHistadine

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pTriEx-NTOM20-mVenus-Zdk3

Plasmid

#81012

Purpose

Expression of Zdk1 anchored to mitochondria

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

Zdk3

Tags

6x His tag, FLAG, NTOM20, and mVenus

Expression

Bacterial, Insect, and Mamm…

Available Since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-Ace2_D92N_E199V-mRuby3

Plasmid

#129257

Purpose

Express Ace2_D92N_E199V-mRuby3 in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Ace2_D92N_E199V-mRuby3

Expression

Mammalian

Mutation

D92N, E199V

Promoter

CAG

Available Since

Aug. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMB1-A-pBAD-dCas9

Plasmid

#190132

Purpose

Level 1 MoClo plasmid containing an arabinose inducible dCas9 for CRISPRi

Depositor

Veronica Godoy-Carter

Article

Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

Insert

dCas9

Use

CRISPR

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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ER-GCaMP6f-AAV

Plasmid

#182501

Purpose

GFAP-ER-GCaMP6f AAV: An AAV5 constructed for ER-GCaMP6f with astrocyte specific GFAP promotor

Depositor

Chris Richards

Article

Aryal et al Anal Chem. 2022 Jan 21. doi: 10.1021/acs

Insert

ER localized P450

Use

AAV

Available Since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2 xDvl-RFP

Plasmid

#29683

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

dishevelled (dvl2.L Frog)

Tags

RFP

Expression

Mammalian

Available Since

June 9, 2011

Availability

Academic Institutions and Nonprofits only
pCAG-GFPmG1

Plasmid

#50444

Purpose

Expression of a EGFP variant in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

Insert

GFPmG1

Expression

Mammalian

Mutation

E143K, N147Q

Promoter

CAG

Available Since

March 20, 2014

Availability

Academic Institutions and Nonprofits only

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pEIAV-SIN6.1 CBLacZ-LucWm1A

Plasmid

#44167

Depositor

John Olsen

Article

Patel et al J Gene Med. 2013 Jan;15(1):51-62. doi: 1

Inserts

LacZ
firefly luciferase

Use

Lentiviral and Luciferase

Promoter

CB, CMV enhancer/chicken beta-actin promoter

Available Since

April 24, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pCA_e-2

Plasmid

#202097

Purpose

Receiver cloning vector for even-2 level constructs. Contains lacZ for blue/white screening.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pET42a-lambdaN+-L+-GSH

Plasmid

#98894

Purpose

Expresses lambdaN+-L+-GST fusion protein for affinity purification with ARiBo method

Depositor

Pascale Legault

Article

Di Tomasso et al Nucleic Acids Res. 2011 Feb;39(3):e18. d

Insert

lambdaN+-L+

Tags

GST tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pT2-Tbait-UAS-Positron-ST

Plasmid

#129266

Purpose

UAS-driven expression of Positron in zebrafish

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Positron-ST

Use

Zebrafish expression

Mutation

D92N, E199V

Available Since

Aug. 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-ClopHensor-H148G-V224L

Plasmid

#46366

Depositor

Daniele Arosio

Article

Mukhtarov et al Front Mol Neurosci. 2013 Apr 18;6:9. doi

Insert

ClopHensor-H148G-V224L

Tags

Strep-II

Expression

Mammalian

Mutation

E2GFP-H148G-V224L is EGFP-T203Y-H148G-V224L

Available Since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only
MAC-C-GFP

Plasmid

#172973

Purpose

MAC-tagged GFP expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

GFP

Expression

Bacterial and Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMB1-A-ptet-dCas9

Plasmid

#190129

Purpose

Level 1 MoClo plasmid containing an aTC inducible dCas9 for CRISPRi

Depositor

Veronica Godoy-Carter

Article

Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

Insert

dCas9

Use

CRISPR

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-luxGold

Plasmid

#229948

Purpose

Mammalian expression of a de novo designed, FRET-based neoLux1.2-mGold fusion bioluminescent probe (max. Em=530nm)

Depositor

Andy Yeh

Article

Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

Insert

luxGold

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-Ace2_D92N_E199V-mNeon-ST

Plasmid

#129261

Purpose

Express soma-localized Ace2_D92N_E199V-mNeon in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Ace2_D92N_E199V-mNeon-ST

Expression

Mammalian

Mutation

D92N, E199V

Promoter

CAG

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMito.iGABASnFR.mRuby3

Plasmid

#112179

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR

Tags

mRuby3

Expression

Mammalian

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 7,580 results for: Ski;