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  3. pCSD_FKBP-C-SpCas9MT3-574-1368-NLS-3XHA-NLS-ZFPTS3-3xFLAG-2xNLS

    Plasmid
    #107300
    Purpose
    Expresses C terminal (574-1368 aa) R1335K mutant SpCas9 fused to FKBP and ZFP-VEGFA-TS3 in mammalian cells
    Depositor
    Scot Wolfe
    Insert
    Truncated SpCas9 (aa 574-1368) fused to FKBP
    Use
    CRISPR
    Tags
    3x Flag, 2x NLS, FKBP, SV40NLS, 3xHA, c-Myc NLS, …
    Expression
    Mammalian
    Mutation
    Truncated SpCas9 (aa 574-1368) with R1335K mutati…
    Promoter
    CMV IE94
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107300-plasmid-map-sequence-id-204631

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  4. pCSD_FKBP-C-SpCas9MT3-574-1368-NLS-3XHA-NLS-ZFPTS2-3xFLAG-2xNLS

    Plasmid
    #107299
    Purpose
    Expresses C terminal (574-1368 aa) R1335K mutant SpCas9 fused to FKBP and ZFP-VEGFA-TS2 in mammalian cells
    Depositor
    Scot Wolfe
    Insert
    Truncated SpCas9 (aa 574-1368) fused to FKBP
    Use
    CRISPR
    Tags
    3x Flag, 2x NLS, FKBP, SV40NLS, 3xHA, c-Myc NLS, …
    Expression
    Mammalian
    Mutation
    Truncated SpCas9 (aa 574-1368) with R1335K mutati…
    Promoter
    CMV IE94
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107299-plasmid-map-sequence-id-204615

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  8. pHL2073

    Plasmid
    #62837
    Purpose
    Lim Lab plasmid: AmpR + PconShortNoHind::glmZ::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1m9::glmS::gfp::T1T2 terminator + ColE1
    Depositor
    Han Lim
    Article
    Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv
    Inserts
    glmZ leader
    T710 hfq leader
    gfp
    Use
    Synthetic Biology
    Tags
    m9::glmS
    Expression
    Bacterial
    Mutation
    Promoter
    pConShortNoHind, pLlacO-1, and pLtetO-1
    Available since
    Aug. 14, 2015
    Availability
    Academic Institutions and Nonprofits only

  9. pHL2075

    Plasmid
    #62838
    Purpose
    Lim Lab plasmid: AmpR + PconShortNoHind::glmZ::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1::glmS::gfp::T1T2 terminator + ColE1
    Depositor
    Han Lim
    Article
    Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv
    Inserts
    glmZ leader
    T710 hfq leader
    gfp
    Use
    Synthetic Biology
    Tags
    glmS
    Expression
    Bacterial
    Mutation
    Promoter
    pConShortNoHind, pLlacO-1, and pLtetO-1
    Available since
    Aug. 14, 2015
    Availability
    Academic Institutions and Nonprofits only

  10. pHL2085

    Plasmid
    #62840
    Purpose
    Lim Lab plasmid: AmpR + PconShortNoHindM8::glmZ::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1::glmS::gfp::T1T2 terminator + ColE1
    Depositor
    Han Lim
    Article
    Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv
    Inserts
    m8::glmZ leader
    T710 hfq leader
    gfp
    Use
    Synthetic Biology
    Tags
    glmS
    Expression
    Bacterial
    Mutation
    Promoter
    pConShortNoHind, pLlacO-1, and pLtetO-1
    Available since
    Aug. 14, 2015
    Availability
    Academic Institutions and Nonprofits only

  11. pHL2117

    Plasmid
    #62842
    Purpose
    Lim Lab plasmid: AmpR + PconNoHindM8::ryhB::Asp terminator + PLtetO-1m9::sodB::gfp::T1T2 terminator + ColE1
    Depositor
    Han Lim
    Article
    Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv
    Inserts
    M8::ryhB leader
    gfp
    Use
    Synthetic Biology
    Tags
    m9::sodB leader
    Expression
    Bacterial
    Mutation
    Promoter
    pConShortNoHind and pLtetO-1
    Available since
    Aug. 14, 2015
    Availability
    Academic Institutions and Nonprofits only

  12. pHL2084

    Plasmid
    #62839
    Purpose
    Lim Lab plasmid: AmpR + PconShortNoHindM8::glmZ::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1m9::glmS::gfp::T1T2 terminator + ColE1
    Depositor
    Han Lim
    Article
    Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv
    Inserts
    m8::glmZ leader
    T710 hfq leader
    gfp
    Use
    Synthetic Biology
    Tags
    m9::glmS
    Expression
    Bacterial
    Mutation
    Promoter
    pConShortNoHind, pLlacO-1, and pLtetO-1
    Available since
    May 19, 2015
    Availability
    Academic Institutions and Nonprofits only

  16. His6-pAG-RTase

    Plasmid
    #214932
    Purpose
    Express fusion proteins of Protein A/G and MMLV reverse transcriptase (25-497) in bacterial systems
    Depositor
    Chuan He
    Article
    Xiao et al Nat Methods. 2024 Jan 10. doi: 10.1038/s
    Insert
    fusion protein of protein A/G and MMLV reverse transcriptase (25-497)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    MMLV RTase (H8Y, D200N, T306K, W313F, T330P, D524…
    Promoter
    T7 promoter
    Available since
    April 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    214932-plasmid-map-sequence-id-426479

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Showing: 1181 - 1200 of 32216 results