8,720 results

1. pCasPA

   Plasmid

   #113347

   Purpose

   Bacterial expression of Cas9 nuclease and λ-Red system in Pseudomonas aeruginosa

   Depositor

   Quanjiang Ji

   Article

   Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-ATF4 mScarlet NLS

   Plasmid

   #115969

   Purpose

   ER-stress sensor - ATF4 translation at ORF3 fluorescent reporter with mScarlet-I

   Depositor

   David Andrews

   Article

   Nougarede et al Assay Drug Dev Technol. 2018 Aug 8. doi:

   Insert

   activating transcription factor 4 (ATF4 Human, Synthetic)
   

   Use

   Lentiviral

   Tags

   HA tag, c-myc NLS, and mScarlet-I

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTRKH3-ermGFP

   Plasmid

   #27169

   Depositor

   Michela Lizier

   Article

   Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

   Insert

   erythromycin ribosomal methylase promoter region fused with modified green fluorescent protein 5
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   presence of an additional 183-bp region at N term…

   Promoter

   Available since

   Feb. 23, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. PresentER-SIINFEKL (mCherry)

   Plasmid

   #102945

   Purpose

   PresentER minigene encoding H2-Kb ligand SIINFEKL (mCherry)

   Depositor

   David Scheinberg

   Article

   Gejman et al Elife. 2018 Nov 30;7. pii: 41090. doi: 1

   Insert

   SIINFEKL (OVAL Chicken, Synthetic)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   OVA257-264

   Promoter

   LTR

   Available since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

5. AbVec1.1-IGKC

   Plasmid

   #80796

   Purpose

   Expression of immunoglobulin light chains in mammalian cells, human kappa constant region

   Depositor

   Hedda Wardemann

   Article

   Tiller et al J Immunol Methods. 2008 Jan 1;329(1-2):1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hCMV IE1 gene promoter

   Available since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGWB401

   Plasmid

   #74795

   Purpose

   Gateway cloning compatible binary vector for simple cloning of gene.

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   No Promoter

   Available since

   July 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLKO5.sgRNA.EFS.GFP

   Plasmid

   #57822

   Purpose

   3rd generation lentiviral Vector for Sp sgRNA delivery without SpCas9, GFP, EFS Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   sgRNA
   EFS
   eGFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR_PGK

   Plasmid

   #79120

   Purpose

   Lentiviral vector for constitutive expression of genes (PGK promoter)

   Depositor

   Wendell Lim

   Article

   Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. p201N Cas9

   Plasmid

   #59175

   Purpose

   Cas9 driven by double 35S, nptII for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

   Depositor

   Wayne Parrott

   Article

   Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

   Inserts

   Cas9
   nptII
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   2x35S and StUbi-3P

   Available since

   Sept. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. dCas9-KRAB

    Plasmid

    #110820

    Purpose

    Conventional dCas9 repressor-dCas9-KRAB

    Depositor

    George Church, Alejandro Chavez

    Article

    Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

    Insert

    dCas9-KRAB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Promoter

    CMV

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3β-FLAG-CBP-HA

    Plasmid

    #32908

    Depositor

    Tso-Pang Yao

    Article

    Zhao et al Mol Cell Biol. 2005 Oct;25(19):8456-64.

    Insert

    CBP (Crebbp Mouse)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAS9counter

    Plasmid

    #107192

    Purpose

    Expresses CAS9 and sgRNA for targeted counterselection in S. aureus

    Depositor

    Steve Salipante

    Article

    Penewit et al MBio. 2018 Feb 20;9(1). pii: mBio.00067-

    Inserts

    Cas9
    sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    prab17

    Available since

    Feb. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNestin-EGFP

    Plasmid

    #38777

    Depositor

    Wei Cui

    Article

    Noisa et al Stem Cell Rev. 2010 Sep;6(3):438-49.

    Insert

    nestin 2nd intron enhancer with TK mini promoter (NES Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRK5-HA-Ubiquitin-KO

    Plasmid

    #17603

    Purpose

    Mammalian expression of HA tagged ubiquitin carrying zero lysines (K0)

    Depositor

    Ted Dawson

    Article

    Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    KO: all lysines mutated to arginines

    Promoter

    Available since

    March 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. mCherry-hALIX

    Plasmid

    #21504

    Depositor

    James Hurley

    Article

    Lee et al Science. 2008 Oct 24. 322(5901):576-80.

    Insert

    ALIX (PDCD6IP Human)
    

    Use

    Tags

    Cherry

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    Sept. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC57-LbNOX

    Plasmid

    #75285

    Purpose

    This plasmid contains human codon optimized sequence of LbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.

    Depositor

    Vamsi Mootha

    Article

    Titov et al Science. 2016 Apr 8;352(6282):231-5. doi

    Insert

    LbNOX
    

    Use

    Tags

    C-terminal FLAG tag with linker

    Expression

    Mutation

    Human codon optimized

    Promoter

    Available since

    May 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRepCap6

    Plasmid

    #110770

    Purpose

    pRepCap6 contains both rep and cap genes from the AAV6 genome, but lacks the viral terminal repeats (Rutledge et al., 1998). It lacks adenovirus helper functions required for stock production.

    Depositor

    David Russell

    Article

    Rutledge et al J Virol. 1998 Jan;72(1):309-19.

    Insert

    AAV6 cap, AAV6 rep genes
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. LifeAct-mNeonGreen

    Plasmid

    #98877

    Purpose

    In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv 160374

    Insert

    LifeAct
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAcBac2.tR4-OMeYRS/GFP*

    Plasmid

    #50831

    Purpose

    baculovirus-based delivery system that enables the efficient incorporation of unnatural amino acids into proteins in mammalian cells

    Depositor

    Peter Schultz

    Article

    Chatterjee et al Proc Natl Acad Sci U S A. 2013 Jul 16;11

    Inserts

    two-copy Tyr tRNA cassette
    EGFP*
    OMeYRS
    two copy Tyr tRNA cassette
    

    Use

    Baculoviral

    Tags

    His, Myc-6xHis, and WPRE

    Expression

    Insect

    Mutation

    Y39TAG and able to charge various unnatural amino…

    Promoter

    CAG, CMV, and U6 and H1

    Available since

    June 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDisCoTune

    Plasmid

    #173713

    Purpose

    Promotes expression of disulfide-rich protein in E. coli T7 expression system. The plasmid encodes expression of the folding factors Erv1p and hPDI controlled by the Ptac promoter.

    Depositor

    Morten Norholm

    Article

    Bertelsen et al Microb Biotechnol. 2021 Nov;14(6):2566-2

    Inserts

    ERV1 (ERV1 Budding Yeast)
    PDI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge