2,339 results

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pEGFP-Sec22b-P33-shR

Plasmid

#208359

Purpose

Mammalian expression of fluorescent N-terminally tagged shRNA resistant Sec22b-P33 mutant

Depositor

Paula Nunes-Hasler

Article

Criado Santos et al Commun Biol. 2023 Oct 4;6(1):1008. doi:

Insert

Sec22b (Sec22b Rat)

Tags

EGFP

Expression

Mammalian

Mutation

four silent mutations (shRNA resistance), 33 aa p…

Promoter

CMV

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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FUp95GW (B7)

Plasmid

#74016

Purpose

lentiviral expression of shRNA resistant Psd95-EGFP fusion

Depositor

Robert Malenka, Oliver Schluter, Weifeng Xu

Article

Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

Insert

Psd95 (DLG4 Rat)

Use

Lentiviral

Expression

Mammalian

Promoter

hUbC

Available Since

April 1, 2016

Availability

Academic Institutions and Nonprofits only

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pORANGE CACNG2-GFP KI

Plasmid

#131504

Purpose

Endogenous tagging of Tarpγ2/Stargazin: Intramolecular (amino acid position: S265)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Cacng2, Cacng2 Mouse, Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-sACt-mCherry

Plasmid

#138216

Purpose

Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; plasma membrane targeted.

Depositor

Jin Zhang

Article

Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

Insert

Lyn-sACt-mCherry (Adcy10 Rat)

Tags

N-terminal targeting sequence from Lyn kinase and…

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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Lyn-iTrkA-mCh-CRY2

Plasmid

#106169

Purpose

mammalian expression of Lyn-tagged intracellular domain of TrkA (aa 450-799) fused to mCherry and CRY2PHR (aa 1-498)

Depositor

Bianxiao Cui

Article

Duan et al ACS Synth Biol. 2018 Jul 20;7(7):1685-16

Insert

Lyn iTrkA mCherry CRY2PHR (CRY2 Rat, Mustard Weed)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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CRY2-mCherry-iTrkA

Plasmid

#106168

Purpose

mammalian expression of CRY2PHR (aa 1-498) fused to mCherry and intracellular domain of TrkA (aa 450-799)

Depositor

Bianxiao Cui

Article

Duan et al ACS Synth Biol. 2018 Jul 20;7(7):1685-16

Insert

CRY2PHR mCherry iTrkA (CRY2 Rat, Mustard Weed)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 VAMP2 R125TAG

Plasmid

#69876

Purpose

express in mammalian cells for unnatural amino acid incorporation into VAMP2-GFP

Depositor

Silvio Rizzoli

Article

Vreja et al ACS Nano. 2015 Nov 24;9(11):11034-41. do

Insert

VAMP2-GFP (Vamp2 Rat)

Use

Synthetic Biology

Tags

GFP

Expression

Mammalian

Mutation

Amber stop codon in the linker between VAMP2 and …

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only
XE117 CAMKII-CS2+

Plasmid

#16737

Depositor

Randall Moon

Article

Kühl et al J Biol Chem. 2000 Apr 28. 275(17):12701-

Insert

CAMKII (Camk2a Rat)

Use

Xenopus expression

Available Since

March 14, 2008

Availability

Academic Institutions and Nonprofits only
RSV CREB M1

Plasmid

#22395

Depositor

Marc Montminy

Article

Gonzalez et al Cell. 1989 Nov 17. 59(4):675-80.

Insert

CREB (Creb1 Rat)

Expression

Mammalian

Mutation

Changed Serine 133 to Alanine

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
pCR3-FLAG-Gephyrin GC

Plasmid

#68818

Purpose

Flag tagged mammalian expression of GC domain

Depositor

Shiva Tyagarajan

Article

Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

Insert

Gephyrin (Gphn Rat)

Tags

3xFLAG

Expression

Mammalian

Mutation

NIL

Promoter

CMV

Available Since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-ERK2 L232A

Plasmid

#8976

Depositor

John Blenis

Article

Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

Insert

ERK2 (Mapk1 Rat)

Tags

HA

Expression

Mammalian

Mutation

L232A

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only
pNCX2

Plasmid

#67148

Purpose

Expresses mRNA of NCX2 (Rat brain)

Depositor

Michela Ottolia, Kenneth Philipson

Article

Li et al J Biol Chem. 1994 Jul 1;269(26):17434-9.

Insert

NCX2

Available Since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

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EYFP-Lysosomes-20

Plasmid

#56595

Purpose

Localization: Lysosome Membrane, Excitation: 513, Emission: 527

Depositor

Michael Davidson

Insert

LAMP1

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pORANGE WASF1-GFP KI

Plasmid

#131502

Purpose

Endogenous tagging of WASP1/WAVE1: C-terminal (amino acid position: STOP codon)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Wasf1 Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pFSW Doc2alpha WT

Plasmid

#128812

Purpose

To make virus expressing Doc2alpha WT

Depositor

Edwin Chapman

Article

Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

Insert

Doc2alpha (Doc2a Rat)

Use

Lentiviral

Tags

IRES-EGFP

Promoter

hSyn (human synapsin I promoter)

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pFSW Doc2beta WT

Plasmid

#128814

Purpose

To make virus expressing Doc2beta WT

Depositor

Edwin Chapman

Article

Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

Insert

Doc2beta (Doc2b Rat)

Use

Lentiviral

Tags

IRES-EGFP

Promoter

hSyn (human synapsin I promoter)

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-LMO4 (L4)

Plasmid

#22964

Depositor

Anirvan Ghosh

Insert

LMO4 (Lmo4 Rat)

Tags

HA

Expression

Mammalian

Available Since

Jan. 29, 2010

Availability

Academic Institutions and Nonprofits only

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pG1-GR526

Plasmid

#1124

Depositor

Susan Lindquist

Article

Li et al Science 2000 Jan 28;287(5453):661-4.

Insert

GR526 (Nr3c1 Rat)

Expression

Yeast

Mutation

GR 526 is a truncated, constitutively active form…

Available Since

June 3, 2005

Availability

Academic Institutions and Nonprofits only
Cav2.2e[Δa10, Δ18a, Δ24a, 31a, 37b, 46]

Plasmid

#26570

Depositor

Diane Lipscombe

Article

Lin et al Neuron. 1997 Jan . 18(1):153-66.

Insert

calcium channel alpha-1B subunit (Cacna1b Rat)

Tags

6XHis and V5

Expression

Mammalian

Mutation

Deletion of exons a10, 18a, and 24a. Contains …

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only
pBactin-Nedd1-mNeonGreen

Plasmid

#196864

Purpose

Expression of neural precursor cell expressed developmentally down-regulated 1 (Need1) fused to mNeonGreen

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

Nedd1-mNeonGreen (Nedd1 Rat)

Expression

Mammalian

Promoter

Chicken bactin (plus Chicken bactin intron)

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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