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Addgene

We narrowed to 9,223 results for: Coli

Showing: 1801 - 1830 of 9223 results
  1. pKP2032

    Plasmid
    #167651
    Purpose
    Express His6-ExbB S43A, S45A, S67A, S80A, S82A, S84A, S98A, S101A, T111A, S112A, S221A, S229A
    Depositor
    Insert
    his6-exbB S43A, S45A, S67A, S80A, S82A, S84A, S98A, S101A, T111A, S112A, S221A, S229A
    Use
    Tags
    Expression
    Bacterial
    Mutation
    ExbB S43A, S45A, S67A, S80A, S82A, S84A, S98A, S1…
    Promoter
    Available since
    July 9, 2021
    Availability
    Academic Institutions and Nonprofits only
  2. pKP372

    Plasmid
    #167593
    Purpose
    Express TonB frameshift of amphipathic helix
    Depositor
    Insert
    tonB fs
    Use
    Tags
    Expression
    Bacterial
    Mutation
    tonB with frameshift of amphipathic helix
    Promoter
    Available since
    May 26, 2021
    Availability
    Academic Institutions and Nonprofits only
  3. pREDCas9

    Plasmid
    #71541
    Purpose
    Constitutive expression of Cas9, inducible expression of the Red recombineering system, and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.
    Depositor
    Inserts
    Cas9
    lambda red genes
    plasmid curing system
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available since
    July 29, 2016
    Availability
    Academic Institutions and Nonprofits only
  4. pCRISPathBrick

    Plasmid
    #65006
    Purpose
    E. coli vector for expression of S. pyogenes dCas9, tracrRNA, and nontargeting CRISPR array with BsaI site for inserting user-defined spacer-repeat bricks
    Depositor
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Constitutive native promoters
    Available since
    May 27, 2015
    Availability
    Academic Institutions and Nonprofits only
  5. PatoL-OG241

    Plasmid
    #72841
    Purpose
    Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Bba_B0034 (iGEM). OG241 backbone.
    Depositor
    Insert
    PatoL
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PatoL
    Available since
    Aug. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
  6. p46Cpf1-OP2

    Plasmid
    #98592
    Purpose
    Produces lambda Red and Cpf1 for recombination and selection. The plasmid is combined with a donor plasmid (with crRNA and template) for rapid genome editing in E.coli.
    Depositor
    Insert
    FnCpf1
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Codon optimized for E. coli
    Promoter
    araBAD
    Available since
    Feb. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
  7. pM9-BetaGal-8C

    Plasmid
    #172489
    Purpose
    Plasmid encoding E. coli BetaGalactosidase with an N-terminal M9 nuclear localization sequence
    Depositor
    Insert
    M9-BetaGal-8C
    Use
    Tags
    6xHis and M9 nuclear localization sequence (from …
    Expression
    Bacterial
    Mutation
    BetaGal has two cysteine mutations (C77A, C1022S)…
    Promoter
    T7
    Available since
    March 7, 2022
    Availability
    Academic Institutions and Nonprofits only
  8. pCarCBADE_ProB(I69E)A

    Plasmid
    #117756
    Purpose
    ProB(I69E)A and codon optimized CarCBADE enzymes (in that order) in pBbaA5k backbone
    Depositor
    Insert
    Glutamate kinase (ProA), mutant pyrroline-5-carboxylate synthase (ProBI69E) from E. coli and codon optimised CarCBADE operon
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLac(UV5)
    Available since
    Dec. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
  9. pK1E-RNAP

    Plasmid
    #48144
    Purpose
    Shuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage K1E under control of the xylose inducible promoter PxylA
    Depositor
    Insert
    rna polymerase K1E
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 21, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 1801 - 1830 of 9223 results