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Plasmid#129719PurposeAAVS1 safe harbor targeting vector expressing miniIAA7-mEGFP tagged SeipinDepositorInsertSeipin (BSCL2 Human)
UseCRISPR and TALEN; Human safe harbor locus (a avs1…TagsminiIAA7-mEGFPExpressionMammalianMutationsilent mutationsPromoterEF1aAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-MPAct-mRuby3-IRES-Puro
Plasmid#155231Purposelentiviral MPAct constructDepositorInsertLentiviral expression of MPAct-mRuby sensor
UseLentiviralTagsmRuby3ExpressionMutationPromoterAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG PSAM4 5HT3 LC IRES GFP
Plasmid#123066PurposeChemogenetic activator expression plasmidDepositorInsertPSAM4 5HT3 LC IRES GFP (Htr3a Mouse)
UseTagsExpressionMammalianMutationL131G Q139L Y217FPromoterCAGAvailable sinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS306-URA3-PHIS3-PfV-GS-Sapphire
Plasmid#116932PurposeYeast integrative vector for the exression of the 40nm GEM under the HIS3 promoterDepositorInsertPfv
UseTagsSapphireExpressionYeastMutationPromoterHIS3Available sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
MSCV-CMV-DsRed-IRES-d1EGFP
Plasmid#41942PurposeRetroviral expression of DsRed in mammalian cellsDepositorInsertDsRed
UseRetroviralTagsExpressionMammalianMutationPromoterTREAvailable sinceMay 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPW3755 : HDR-HsIRE1a-10xGS-HaloTag
Plasmid#185677PurposeHDR template used for endogenous CRISPR editing of ERN1 gene, inserting a C-terminal HaloTag.DepositorInsertERN1 homology arms (ERN1 Synthetic, Human)
UseCRISPRTagsHaloTagExpressionMutationPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS305-Leu2-PINO4-PfV-GS-Sapphire
Plasmid#116930PurposeYeast integrative vector for the exression of the 40nm GEM under the INO4 promoterDepositorInsertPfv
UseTagsSapphireExpressionYeastMutationPromoterINO4Available sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-dCasRx-RBM25-IRES-BleoR
Plasmid#221002PurposeStable expression of dCasRx-RBM25 when co-transfected with piggyBac transposase. Zeocin selection. CAG promoter. Use with lentiviral gRNA delivery (Addgene #138150) to stably modulate splicing.DepositorInsertdCasRx-RBM25-IRES-BleoR
UseCRISPRTagsNLS and NLS-HAExpressionMammalianMutationPromoterCAGAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-human-Caspase-4
Plasmid#183356PurposeExpression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorInsertCaspase-4 (CASP4 Human)
UseRetroviralTagsMycExpressionMutationPromoterMESVAvailable sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-HA-KORD-IRES-mCitrine
Plasmid#65418PurposeAAV packaging vector for CamKIIa driven KOR DREADD expressionDepositorInsertCaMKIIa-HA-KORD-IRES-mCitrine (OPRK1 Human)
UseAAVTagsHA and mCitrineExpressionMutationPromoterCaMKIIaAvailable sinceJune 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPW3754 : pUC-SpCas9_gRNA-HsIRE1-Cterm
Plasmid#185676PurposeSpCas9 and gRNA targeting the C-terminus of HsIRE1DepositorInsertERN1 gRNA (ERN1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
hPAX7-3xHA-IRES-hrGFP (#400)
Plasmid#78338PurposeOverexpression of human PAX7 ORF with a 3xHA C-terminal tag and an IRES-hrGFP reporterDepositorInsertPAX7 (PAX7 Human)
UseTags3x HA tag and IRES-hrGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
Gβ-2A-cpV-Gγ2-IRES-Gαi2-mTq2
Plasmid#69624PurposeA FRET sensor for Galphai2 activation, encoded on a single plasmid.DepositorInsertsGbeta-2A-cpV-Ggamma2
Galphai2-mTurquoise2
UseTagsciruclar permutated Venus (cp173V) and mTurquoise…ExpressionMammalianMutationPromoterCMV and CMV (IRES)Available sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB_ATP1.0mut-IRES-mCherry-CAAX
Plasmid#167583PurposeExpress the ATP-insensitive control sensor GRAB_ATP1.0mut in mammalian cellsDepositorInsertATP-insensitive sensor GRAB_ATP1.0mut (control sensor) (P2RY1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
TurboID-MCS MSCV-IRES-GFP
Plasmid#207958PurposeRetroviral vector for cloning a gene of interest with a glycine-serine linker and TurboID on the N-terminusDepositorInsertTurboID
UseRetroviral; Turboid biotin proximity ligaseTagsTurboIDExpressionMammalianMutationPromoterAvailable sinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWPI-IRES-Bla-Ak-ACE2-TMPRSS2
Plasmid#154983PurposeLentiviral vector expressing Human ACE2 and TMPRSS2DepositorUseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-IRESWT-Gbetagamma ONE-GO
Plasmid#204339PurposeGPCR/G protein BRET biosensorDepositorInsertGbetagamma ONE-GO
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
TRE-KRAB-dCas9-IRES-GFP
Plasmid#85556Purpose2nd generation lentiviral vector expressing a KRAB-dCas9 fusion protein and GFPDepositorInsertKRAB-dCas9-IRES-GFP
UseCRISPR and LentiviralTags2x NLS, HA Tag, and KRAB domainExpressionMammalianMutationPromoterTRE3GAvailable sinceSept. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLVX-IRES-hygro-EGFP-DFCP1
Plasmid#174651PurposeVector for overexpression of EGFP-tagged DFCP1 with IRES for hygromycin selection.DepositorInsertEGFP-DFCP1 (ZFYVE1 Human)
UseLentiviralTagsEGFPExpressionMutationPromoterCMVAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOTTC385 - pAAV CMV-IE IRES EGFP
Plasmid#102936PurposeAn AAV vector that expresses IRES EGFP under the CMV-IE promoterDepositorInsertEGFP
UseAAVTagsExpressionMammalianMutationPromoterCMV-IEAvailable sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only