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pDONR207 SARS-CoV-2 NSP3

Plasmid

#141257

Purpose

Gateway-compatible Entry vector, with insert of NSP3 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP3 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

12 Articles

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pNCS-mClover3

Plasmid

#74236

Purpose

Constitutive expression of mClover3 in bacteria

Depositor

Michael Lin

Publication

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Insert

mClover3

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLenti-MetGFP

Plasmid

#37560

Depositor

David Rimm

Insert

MET (MET Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

22 Articles

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pAAV-FLEX-GFP

Plasmid

#28304

Purpose

Cre-dependent AAV-mediated expression of GFP under the CAG promoter

Depositor

Edward Boyden

Insert

GFP

Use

AAV and Cre/Lox; Adeno-associated virus

Expression

Mammalian

Mutation

N/A

Promoter

CAG

Available since

July 7, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

47 Articles

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pcDNA3 Flag beta-1-adrenergic-receptor

Plasmid

#14698

Depositor

Robert Lefkowitz

Publication

Tang et al Proc Natl Acad Sci U S A. 1999 Oct 26. 9

Insert

B1AR (ADRB1 Human)

Tags

Flag

Expression

Mammalian

Available since

June 19, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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HDAC8 Flag

Plasmid

#13825

Depositor

Eric Verdin

Publication

Waltregny et al FASEB J. 2005 Jun . 19(8):966-8.

Insert

HDAC8 (HDAC8 Human)

Tags

flag

Expression

Mammalian

Available since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pGEXTK-Pak1 70-117

Plasmid

#12217

Depositor

Jonathan Chernoff

Insert

Pak1 (PAK1 Human)

Tags

GST

Expression

Bacterial

Mutation

PBD (p21-binding domain), aa 70-117

Available since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

26 Articles

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pLB

Plasmid

#11619

Purpose

pLB is a modification of pLL3.7 (Addgene#11795). Genetic elements known to prevent epigenetic silencing were added.

Depositor

Stephan Kissler

Publication

Kissler et al Nat Genet. 2006 Apr . 38(4):479-83.

Type

Empty backbone

Use

Cre/Lox, Lentiviral, and RNAi

Tags

GFP

Expression

Mammalian

Promoter

mouse U6

Available since

July 19, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

30 Articles

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Rictor_2 shRNA

Plasmid

#1854

Depositor

David Sabatini

Publication

Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

Insert

Rictor (RICTOR Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

April 7, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pMBP-LbCas12a

Plasmid

#113431

Purpose

Expresses 10xHis-MBP fused to LbCas12a

Depositor

Jennifer Doudna

Publication

Chen et al Science. 2018 Apr 27;360(6387):436-439.

Insert

LbCas12a

Tags

MBP

Expression

Bacterial

Promoter

T7

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

19 Articles

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pCLBW cox8 EGFP mCherry

Plasmid

#78520

Purpose

Fluorescent mitophagy reporter

Depositor

David Chan

Publication

Rojansky et al Elife. 2016 Nov 17;5. pii: e17896. doi:

Insert

none

Use

Retroviral

Tags

cox8 EGFP mCherry

Expression

Mammalian

Promoter

CMV

Available since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

32 Articles

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pAAV-flex-taCasp3-TEVp

Plasmid

#45580

Depositor

Nirao Shah, Jim Wells

Publication

Yang et al Cell. 2013 May 9;153(4):896-909. doi: 10

Has service

AAV1, AAV5, and AAV8

Inserts

Caspase 3 (CASP3 Synthetic, Human)
Tobacco Etch Virus protease

Use

AAV; Adeno associated viral vector

Mutation

Linker replaced with a TEV protease cleavage site

Promoter

EF1a

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

74 Articles

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pEGFP-IQGAP1

Plasmid

#30112

Depositor

David Sacks

Publication

Ren et al J Biol Chem. 2005 Oct 14. 280(41):34548-

Insert

IQGAP1 (IQGAP1 Human)

Tags

tandem EGFP

Expression

Mammalian

Promoter

CMV

Available since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pAAV-Ef1a-DIO EYFP

Plasmid

#27056

Depositor

Karl Deisseroth

Has service

AAV Retrograde, AAV1, AAV2, AAV5, and AAV9

Insert

EYFP

Use

AAV

Expression

Mammalian

Available since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

203 Articles

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pENTR4-FLAG (w210-2)

Plasmid

#17423

Purpose

Gateway entry vector for N-term FLAG tag

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning

Tags

FLAG

Expression

Mammalian

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

19 Articles

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p3258 pCMV hUBC9 wt HA

Plasmid

#14438

Depositor

Peter Howley

Publication

Yasugi et al Nucleic Acids Res. 1996 Jun 1. 24(11):20

Insert

UBC9 (UBE2I Human)

Tags

HA

Expression

Mammalian

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pX330 p53

Plasmid

#59910

Purpose

pX330 backbone expressing sgRNA targeting p53 to edit mouse p53. Expresses Cas9 from CBh promoter

Depositor

Tyler Jacks

Publication

Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1

Insert

sgRNA targeting p53 (Trp53 Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

32 Articles

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pcDNA5-FRT/TO-eGFP-53BP1

Plasmid

#60813

Purpose

mammalian expression vector

Depositor

Daniel Durocher

Publication

Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

Insert

53BP1 (TP53BP1 Human)

Tags

eGFP

Expression

Mammalian

Available since

Dec. 9, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

18 Articles

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IF-GFP-ATRX

Plasmid

#45444

Purpose

ATRX expression vector

Depositor

Michael Dyer

Publication

Valle-Garcia et al Springerplus. 2014 May 2;3:222. doi: 10.

Insert

ATRX (ATRX Mouse, Human)

Tags

GFP and HA

Expression

Bacterial and Mammalian

Mutation

isoform 2, missing codon E124

Promoter

PCMV

Available since

May 24, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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HA-hULK1

Plasmid

#31963

Depositor

Do-Hyung Kim

Publication

Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

Insert

ULK1 (ULK1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV, SP6

Available since

Sept. 1, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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