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Showing: 3051 - 3100 of 3206 results

  3. pBA950

    Plasmid
    #122239
    Purpose
    CROP-seq vector optimized for sgRNA expression and CRISPRi activity. This vector expresses a eGFP-NT2 sgRNA with modified U6 promotor and sgRNA constant region.
    Depositor
    Jonathan Weissman
    Article
    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.
    Insert
    BFP selectable marker, plus a modified mU6 promoter and sgRNA constant region for optimized CRISPRi activity
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    122239-plasmid-map-sequence-id-292012

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  9. CIRTS-control

    Plasmid
    #213788
    Purpose
    CIRTS RNA targeting system for targeted knockdown in neurons. CIRTS-Calm3 and scramble control guide RNA is expressed under human Syn1 and U6 promoter, respectively.
    Depositor
    Timothy Bredy
    Article
    Liau et al Nat Commun. 2023 Nov 22;14(1):7616. doi:
    Insert
    CIRTS-Calm3-U6-control gRNA
    Use
    Lentiviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Syn1, U6
    Available since
    Feb. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    213788-plasmid-map-sequence-id-424349

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  10. CIRTS-Gas5

    Plasmid
    #213172
    Purpose
    CIRTS RNA targeting system for targeted knockdown in neurons. CIRTS-Calm3 and Gas5 guide RNA is expressed under human Syn1 and U6 promoter, respectively.
    Depositor
    Timothy Bredy
    Article
    Liau et al Nat Commun. 2023 Nov 22;14(1):7616. doi:
    Insert
    CIRTS-Calm3-U6-Gas5 gRNA
    Use
    Lentiviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Syn1, U6
    Available since
    Feb. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    213172-plasmid-map-sequence-id-424348

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  11. pLV-SFFV-PIK3CA-WPRE-UbC-Emerald

    Plasmid
    #203797
    Purpose
    Lentiviral vector expressing human phosphoinositide 3-kinase catalytic subunit alpha (PIK3CA) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Bart Nieuwenhuis, Richard Eva
    Article
    Nieuwenhuis et al EMBO Mol Med. 2020 Aug 7;12(8):e11674. d
    Inserts
    PIK3CA (PIK3CA Human)
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    203797-plasmid-map-sequence-id-401572

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  13. pLV-SFFV-PIK3CA H1047R-WPRE-UbC-Emerald

    Plasmid
    #203798
    Purpose
    Lentiviral vector expressing human PIK3CA H1047R under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Bart Nieuwenhuis, Richard Eva
    Article
    Nieuwenhuis et al EMBO Mol Med. 2020 Aug 7;12(8):e11674. d
    Inserts
    PIK3CA (PIK3CA Human)
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    H1047R
    Promoter
    Available since
    July 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    203798-plasmid-map-sequence-id-401509

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  14. CIRTS-YTHDF2-control

    Plasmid
    #216857
    Purpose
    CIRTS RNA targeting system for targeted knockdown of m6A-containing transcript at the synapse. CIRTS-YTHDF2-Calm3 and scramble gRNA is expressed under human Syn1 and U6 promoter, respectively.
    Depositor
    Timothy Bredy
    Article
    Madugalle et al J Neurosci. 2023 Oct 25;43(43):7084-7100
    Insert
    CIRTS-YTHDF2-Calm3-U6-control gRNA
    Use
    Lentiviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Syn1, U6
    Available since
    Oct. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    216857-plasmid-map-sequence-id-448432

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  15. CIRTS-YTHDF2-Malat1

    Plasmid
    #216858
    Purpose
    CIRTS RNA targeting system for targeted knockdown of m6A-containing Malat1 at the synapse. CIRTS-YTHDF2-Calm3 and Malat1 gRNA is expressed under human Syn1 and U6 promoter, respectively.
    Depositor
    Timothy Bredy
    Article
    Madugalle et al J Neurosci. 2023 Oct 25;43(43):7084-7100
    Insert
    CIRTS-YTHDF2-Calm3-U6-Malat1 gRNA
    Use
    Lentiviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Syn1, U6
    Available since
    Aug. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216858-plasmid-map-sequence-id-443348

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  16. pLKO.3G

    Plasmid
    #14748
    Depositor
    Christophe Benoist, Diane Mathis
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 5, 2007
    Availability
    Academic Institutions and Nonprofits only
    14748-plasmid-map-sequence-id-352429

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  17. pAlbumin

    Plasmid
    #22037
    Depositor
    Didier Trono
    Insert
    partial sequence of human albumine
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2009
    Availability
    Academic Institutions and Nonprofits only
    22037-plasmid-map-sequence-id-323904

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  18. pTitin

    Plasmid
    #26428
    Depositor
    Didier Trono
    Insert
    partial sequence of mouse Titin
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 18, 2010
    Availability
    Academic Institutions and Nonprofits only
    26428-plasmid-map-sequence-id-11494

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  19. pLLU2G

    Plasmid
    #21620
    Depositor
    David Garbers
    Type
    Empty backbone
    Use
    Cre/Lox, Lentiviral, and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 16, 2009
    Availability
    Academic Institutions and Nonprofits only
    21620-plasmid-map-sequence-id-9928

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  20. pLLU2G-Dazl1

    Plasmid
    #21621
    Depositor
    David Garbers
    Insert
    Dazl shRNA-1
    Use
    Cre/Lox, Lentiviral, and RNAi
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 25, 2009
    Availability
    Academic Institutions and Nonprofits only
    21621-plasmid-map-sequence-id-9929

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  21. pLLU2G-Dazl2

    Plasmid
    #21622
    Depositor
    David Garbers
    Insert
    Dazl shRNA-2
    Use
    Cre/Lox, Lentiviral, and RNAi
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 25, 2009
    Availability
    Academic Institutions and Nonprofits only
    21622-plasmid-map-sequence-id-9930

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  22. pCIG3_mCherry_Rab5_DN

    Plasmid
    #75111
    Purpose
    expression of mCherry-Rab5 DN and EGFP
    Depositor
    Felicia Goodrum
    Insert
    RAB5A (RAB5A Human)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    DN S34N
    Promoter
    CMV
    Available since
    May 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    75111-plasmid-map-sequence-id-142031

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  23. pRosettav2

    Plasmid
    #115557
    Purpose
    To determine correct antibiotic selection conditions in mammalian cells; standard for titering experiments
    Depositor
    John Doench
    Insert
    Hygromycin-Blasticidin-Puromycin-EGFP
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    115557-plasmid-map-sequence-id-226246

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  24. pDECKO_mCherry_AAVS1

    Plasmid
    #141212
    Purpose
    pDECKO_mCherry_AAVS1_186. Targeting, non-essential locus control
    Depositor
    Rory Johnson
    Insert
    gRNA
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    141212-plasmid-map-sequence-id-299605

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  25. CROPseq-EFS-SpCas9-P2A-blast

    Plasmid
    #106477
    Purpose
    single-vector CROPseq system with blasticidin resistance selection for use in the CROPseq CRISPR knockout screens
    Depositor
    Alex Hewitt
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Available since
    April 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    106477-plasmid-map-sequence-id-207983

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  26. pCDH-hMALAT1 deletion 11 PARP1 binding sites

    Plasmid
    #159327
    Purpose
    Mammalian cell expression of human MALAT1 with deleted PARP1 binding sites. Contains CopGFP marker.
    Depositor
    Jianjun Zhao
    Insert
    MALAT1 (MALAT1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Deletion 11 PARP1 binding sites
    Promoter
    Available since
    Sept. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    159327-plasmid-map-sequence-id-311872

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  27. pCVL TLR 3.1 (Sce target) Ef1a iRFP Puro

    Plasmid
    #50417
    Purpose
    Fluorescent reporter for DNA cleavage/repair in mammalian cells expressing iRFP with selectable puromycin marker.
    Depositor
    Andrew Scharenberg
    Inserts
    eGFP with I-Sce I TS
    +3 mCherry
    Use
    Lentiviral
    Tags
    T2A dislinker and iRFP
    Expression
    Mammalian
    Mutation
    embedded I-Sce I TS from 163-185
    Promoter
    SFFV
    Available since
    Jan. 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    50417-plasmid-map-sequence-id-75349

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  31. pHRSV_CHARMS

    Plasmid
    #233920
    Purpose
    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS
    Depositor
    Marcus Taylor
    Article
    Lichtenstein et al bioRxiv 2024.05.08.593169
    Insert
    CHARMS
    Use
    Lentiviral
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    SV40
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233920-plasmid-map-sequence-id-469222

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  34. pHRSV_CHARMS-TIR

    Plasmid
    #233919
    Purpose
    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS): CHARMS-TIR
    Depositor
    Marcus Taylor
    Article
    Lichtenstein et al bioRxiv 2024.05.08.593169
    Insert
    CHARMS-TIR
    Use
    Lentiviral
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    SV40
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233919-plasmid-map-sequence-id-469221

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  40. pHRSV_CHARMS-3xAla

    Plasmid
    #233921
    Purpose
    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-3xAla (mutated TRAF6 binding site)
    Depositor
    Marcus Taylor
    Article
    Lichtenstein et al bioRxiv 2024.05.08.593169
    Insert
    CHARMS-3xAla
    Use
    Lentiviral
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    SV40
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233921-plasmid-map-sequence-id-469220

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  41. pHRSV_CHARMS-DHF

    Plasmid
    #233922
    Purpose
    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-DHF (Designed Helical Filament)
    Depositor
    Marcus Taylor
    Article
    Lichtenstein et al bioRxiv 2024.05.08.593169
    Insert
    CHARMS-DHF
    Use
    Lentiviral
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    SV40
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233922-plasmid-map-sequence-id-469215

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  42. pHRSV_CHARMS-1x

    Plasmid
    #233923
    Purpose
    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-1x which contains only 1 TRAF6 binding motif
    Depositor
    Marcus Taylor
    Article
    Lichtenstein et al bioRxiv 2024.05.08.593169
    Insert
    CHARMS-1x
    Use
    Lentiviral
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    SV40
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233923-plasmid-map-sequence-id-469219

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  43. pHRSV_CHARMS-3x

    Plasmid
    #233925
    Purpose
    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-5x which contains 3 TRAF6 binding motifs
    Depositor
    Marcus Taylor
    Article
    Lichtenstein et al bioRxiv 2024.05.08.593169
    Insert
    CHARMS-3x
    Use
    Lentiviral
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    SV40
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233925-plasmid-map-sequence-id-469216

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  44. pHRSV_CHARMS-5x

    Plasmid
    #233927
    Purpose
    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-5x which contains 5 TRAF6 binding motifs
    Depositor
    Marcus Taylor
    Article
    Lichtenstein et al bioRxiv 2024.05.08.593169
    Insert
    CHARMS-5x
    Use
    Lentiviral
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    SV40
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233927-plasmid-map-sequence-id-469202

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  45. pHRSV_CHARMS_bDD

    Plasmid
    #233931
    Purpose
    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-dDD contains the bacterial death domain from Nostoc.
    Depositor
    Marcus Taylor
    Article
    Lichtenstein et al bioRxiv 2024.05.08.593169
    Insert
    CHARMS-bDD
    Use
    Lentiviral
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    SV40
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233931-plasmid-map-sequence-id-469205

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  46. pHRSV_CHARMS-11xTA

    Plasmid
    #233932
    Purpose
    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-11xTAcontains 11 TA repeats in place of the death domain
    Depositor
    Marcus Taylor
    Article
    Lichtenstein et al bioRxiv 2024.05.08.593169
    Insert
    CHARMS-11xTA
    Use
    Lentiviral
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    SV40
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233932-plasmid-map-sequence-id-469197

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  47. pHRSV_CHARMS-15xTA

    Plasmid
    #233933
    Purpose
    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-15xTAcontains 15 TA repeats in place of the death domain
    Depositor
    Marcus Taylor
    Article
    Lichtenstein et al bioRxiv 2024.05.08.593169
    Insert
    CHARMS-15xTA
    Use
    Lentiviral
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    SV40
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233933-plasmid-map-sequence-id-469214

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  48. pHRSV_CHARMS-17xTA

    Plasmid
    #233934
    Purpose
    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-17xTAcontains 17 TA repeats in place of the death domain
    Depositor
    Marcus Taylor
    Article
    Lichtenstein et al bioRxiv 2024.05.08.593169
    Insert
    CHARMS-17xTA
    Use
    Lentiviral
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    SV40
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233934-plasmid-map-sequence-id-469193

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  49. pHRSV_CHARMS-20xTA

    Plasmid
    #233935
    Purpose
    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-10xTAcontains 20 TA repeats in place of the death domain
    Depositor
    Marcus Taylor
    Article
    Lichtenstein et al bioRxiv 2024.05.08.593169
    Insert
    CHARMS-20xTA
    Use
    Lentiviral
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    SV40
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233935-plasmid-map-sequence-id-469213

    Enlarge

Showing: 3051 - 3100 of 3206 results