We narrowed to 8,394 results for: Dos;

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pCMV-Myc-GFP

Plasmid

#83375

Purpose

Expression of Myc tagged GFP

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

Myc-GFP

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pHIP_RNA1

Plasmid

#61259

Purpose

Orsay virus RNA1 genome segment expression plasmid for virus reverse genetics

Depositor

David Wang

Article

Jiang et al J Virol. 2014 Oct 15;88(20):11774-81. do

Insert

Orsay RNA1

Expression

Worm

Promoter

heat shock promoter

Available Since

Jan. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pHIP_RNA2

Plasmid

#61260

Purpose

Orsay virus RNA2 genome segment expression plasmid for virus reverse genetics

Depositor

David Wang

Article

Jiang et al J Virol. 2014 Oct 15;88(20):11774-81. do

Insert

Orsay RNA2

Expression

Worm

Promoter

heat shock promoter

Available Since

Jan. 8, 2015

Availability

Academic Institutions and Nonprofits only

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His6TEV-UbG76C

Plasmid

#75299

Purpose

Bacteria expression plasmid for Ubiquitin G76C with removable His6 tag by TEV protease

Depositor

Cynthia Wolberger

Article

Morgan et al Science. 2016 Feb 12;351(6274):725-8. do

Insert

human Ubiquitin mutant G76 to C

Tags

His

Expression

Bacterial

Mutation

changed Glycine 76 to Cystine

Promoter

T7

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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H3K9me3Probe_FullLength

Plasmid

#186463

Purpose

Expresses GFP tagged H3K9me3 recognition motif in mammalian cells.

Depositor

Chongli Yuan

Article

Mendonca et al Cytometry A. 2022 Apr;101(4):339-350. do

Insert

dimeric CDY1 (CDY1 Human)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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VV073: 1xCox8 - GCaMP5G in fubi

Plasmid

#58509

Purpose

Expresses the calcium sensor GCaMP5G in the mitochondria

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

1xCox8 - GCaMP5G

Use

Lentiviral

Expression

Mammalian

Promoter

a-CamKII

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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hUbc13

Plasmid

#51131

Purpose

human ubc13, E2 for synthesis k63 linked ubiquitin chain

Depositor

Cynthia Wolberger

Article

Wiener et al Nature. 2012 Feb 22;483(7391):618-22. do

Insert

ubc13 (UBE2N Human)

Tags

His6 and SUMO

Expression

Bacterial

Promoter

T7

Available Since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
pHR SFFVp Phy-mCherry-CAAX

Plasmid

#50839

Purpose

Optogenetic PI3K activation system; membrane component

Depositor

Wendell Lim

Article

Toettcher et al Nat Methods. 2011 Sep 11;8(10):837-9. do

Insert

PhyB 1-908

Use

Lentiviral and Synthetic Biology

Tags

mCherry

Promoter

SFFV

Available Since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only

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ecAT3.10

Plasmid

#107215

Purpose

Mammalian expression plasmid to target the ecAT3.10 extracellular ATP sensor to the cell surface.

Depositor

Mathew Tantama

Article

Conley et al PLoS One. 2017 Nov 9;12(11):e0187481. do

Insert

ecAT3.10

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pAd5-B6/7 ∆E3-GFP

Plasmid

#179202

Purpose

A block for the AdenoBuilder genome assembly system. The insert consolidates the regions present in pAd5-B6∆E3-GFP and pAd5-B7.

Depositor

Fred Bunz

Article

Jang et al STAR Protoc. 2022 Jan 20;3(1):101123. do

Insert

Modified block 6/7, with deletion in E3 and insertion of GFP cassette

Use

Adenoviral

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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His6-SUMO-pET28a-EfaCas1

Plasmid

#112793

Purpose

To express enterococcus faecalis Cas1 protein in E. coli

Depositor

Ailong Ke

Article

Xiao et al Nature. 2017 Oct 5;550(7674):137-141. do

Insert

EfaCas1

Tags

His6-SUMO

Expression

Bacterial

Available Since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

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Lenti-EF1alpha-dCas9-p300_Blast

Plasmid

#192653

Purpose

3rd generation lenti vector encoding dCas9-p300 with 2A Blast resistance marker

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

dCas9-p300

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A; H840A in Cas9

Promoter

EF1a

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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Anep8_Cas9

Plasmid

#117169

Purpose

Expressed Cas9 in Aspergillus with LIC tags for easy gRNA cloning

Depositor

Adrian Tsang

Article

Song et al PLoS One. 2018 Aug 24;13(8):e0202868. do

Type

Empty backbone

Use

CRISPR and Synthetic Biology; Filamentous fungi e…

Promoter

Pki

Available Since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pSR83

Plasmid

#90211

Purpose

Expresses the response regulator OmpR, and expresses mCherry from the Cph8-OmpR regulated PompF-146 promoter

Depositor

Jeffrey Tabor

Article

Olson et al Mol Syst Biol. 2017 Apr 24;13(4):926. do

Inserts

OmpR (ompR E. coli)
mCherry

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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PEP101E

Plasmid

#97387

Purpose

binary construct of cytosolic sfGFP1-10 for plant

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

sfGFP1-10OPT

Expression

Plant

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pFLAG/HA-MOV10

Plasmid

#10976

Depositor

Thomas Tuschl

Article

Meister et al Curr Biol. 2005 Dec 6;15(23):2149-55. do

Insert

MOV10 (MOV10 Human)

Tags

FLAG and HA

Expression

Mammalian

Available Since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

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MXS_PGK::ZeoR-bGHpA

Plasmid

#62442

Purpose

MXS_chaining vector with PGK::ZeoR-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

resistance cassette against Zeocin with PGK Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pUC19-TgKI-MCS-eGFP_Y40N-MCS-polyA

Plasmid

#174025

Purpose

This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

Depositor

Michel Bagnat

Article

Levic et al Development. 2021 Sep 8. pii: 272090. do

Insert

eGFP

Use

Zebrafish knock-in tagging

Mutation

Y40N

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pET11a+CYP119

Plasmid

#66131

Purpose

Expresses the wild type of CYP119

Depositor

Teruyuki Nagamune

Article

Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

Insert

CYP119 (cyp119 )

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pSTARR-seq_human

Plasmid

#71509

Purpose

humanSTARR-seq screening vector with SCP1 core promoter

Depositor

Alexander Stark

Article

Arnold et al Science. 2013 Mar 1;339(6123):1074-7. do

Type

Empty backbone

Use

Humanstarr-seq screening vector

Tags

sgGFP (SuperGlo, Qbiogene, Inc)

Expression

Mammalian

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only