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pLentiCRISPRv2-Stuffer-HepmCherry

Plasmid

#192825

Purpose

Contains stuffer sequence into which sgRNAs can be cloned and expresses mCherry from hepatocyte-specific promoter

Depositor

Kristin Knouse

Article

Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

Insert

mCherry

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Hepatocyte-specific promoter (HS-CRM8-TTRmin modu…

Available since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pLX-AS

Plasmid

#188870

Purpose

Compact T-DNA binary vector of the pLX series suitable for assembly of plant virus infectious clones and their Agrobacterium-mediated delivery to plants

Depositor

Fabio Pasin

Article

Pasin et al STAR Protoc. 2022 Dec 16;3(4):101716. do

Insert

none

Use

Synthetic Biology; Binary t-dna vector

Tags

Expression

Plant

Mutation

Promoter

cauliflower mosaic virus (CaMV) 35S promoter

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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His6-SUMO-pET28a-EfaCas1

Plasmid

#112793

Purpose

To express enterococcus faecalis Cas1 protein in E. coli

Depositor

Ailong Ke

Article

Xiao et al Nature. 2017 Oct 5;550(7674):137-141. do

Insert

EfaCas1

Use

Tags

His6-SUMO

Expression

Bacterial

Mutation

Promoter

Available since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO SwiChRca-TS-EYFP

Plasmid

#55631

Purpose

Cre-Dependent Chloride Channel

Depositor

Karl Deisseroth

Article

Berndt et al Science. 2014 Apr 25;344(6182):420-4. do

Insert

iC1C2-TS-EYFP

Use

AAV and Cre/Lox

Tags

EYFP

Expression

Mammalian

Mutation

C128A

Promoter

Ef1a

Available since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pET30b-7Y-SrtA

Plasmid

#105601

Purpose

expresses 7Y sortase A variant in E.coli

Depositor

Stephanie Dougan

Article

Jeong et al PLoS One. 2017 Dec 4;12(12):e0189068. do

Insert

S.aureus Sortase A, 7Y mutant

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pDIV296

Plasmid

#204687

Purpose

AMA1 plasmid with nat1 resistance marker

Depositor

Uffe Mortensen

Article

Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

Insert

nat1

Use

Fungal expression

Tags

Expression

Bacterial

Mutation

Promoter

Aspergillus nidulans trpC promoter

Available since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pCR-U6-gRNA-TRE3G-TdTomato

Plasmid

#192658

Purpose

A plasmid encoding TRE3G sgRNA and TRE3G-promoter-TdTomato

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

TdTomato

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6, TRE3G

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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iMbody_pSANG10-3F

Plasmid

#184496

Purpose

Expresses iMbody-6xHis-3xFLAG in BL21(DE3) bacteria

Depositor

Marcel Dinger

Article

Zeraati et al STAR Protoc. 2023 Nov 21;4(4):102729. do

Insert

iMbody

Use

Tags

3xFLAG, 6xHis, and pelB leader

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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MXS_CMV::PuroR-bGHpA

Plasmid

#62439

Purpose

MXS_chaining vector with CMV::PuroR-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

resistance cassette against Puromycin with CMV Promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pTORGm43GWR

Plasmid

#112905

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin. Contains additional cassette for constitutive expression of tdTomato.

Depositor

Sebastian Schornack

Article

Evangelisti et al BMC Microbiol. 2019 Nov 27;19(1):265. do

Type

Empty backbone

Use

Oomycete expression

Tags

Expression

Mutation

Promoter

Available since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pTORGm43GWC

Plasmid

#112903

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin. Contains additional cassette for constitutive expression of mTFP1.

Depositor

Sebastian Schornack

Article

Evangelisti et al BMC Microbiol. 2019 Nov 27;19(1):265. do

Type

Empty backbone

Use

Oomycete expression

Tags

Expression

Mutation

Promoter

Available since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pTORGm43GWF

Plasmid

#112904

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin. Contains additional cassette for constitutive expression of mWasabi.

Depositor

Sebastian Schornack

Article

Evangelisti et al BMC Microbiol. 2019 Nov 27;19(1):265. do

Type

Empty backbone

Use

Oomycete expression

Tags

Expression

Mutation

Promoter

Available since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pUC19-TgKI-MCS-eGFP_Y40N-MCS-polyA

Plasmid

#174025

Purpose

This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

Depositor

Michel Bagnat

Article

Levic et al Development. 2021 Sep 8. pii: 272090. do

Insert

eGFP

Use

Zebrafish knock-in tagging

Tags

Expression

Mutation

Y40N

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-EF1alpha-scFv-VP64_Blast

Plasmid

#192655

Purpose

3rd generation lenti vector encoding scFv-VP64 with 2A Blast resistance marker

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

scFv-VP64

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAC14

Plasmid

#197408

Purpose

Honey bee gut symbiont broad-host range vector with constitutive GFP, ampR and pTFC-FC2 replicon

Depositor

Yolanda Schaerli

Article

Chhun et al PLoS Biol. 2024 Mar 5;22(3):e3002523. do

Insert

pTF-FC2 replicon

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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sgDonor_lentiGuide

Plasmid

#184168

Purpose

LentiGuide-puro vector expressing the sgRNA necessary for linearization of the donor cassette plasmid.

Depositor

Ophir Shalem

Article

Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

Insert

sgDonor

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.Control

Plasmid

#128749

Purpose

Expresses control gRNA

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

Control sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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p3E-APEX2

Plasmid

#108894

Purpose

Multisite gateway vector for 3' tagging with APEX2

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

APEX2

Use

Multisite gateway

Tags

Expression

Mutation

Promoter

Available since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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pMMB67EH-YFP

Plasmid

#90104

Purpose

YFP control expression plasmid

Depositor

Samuel Miller

Article

Christen et al Science. 2010 Jun 4;328(5983):1295-7. do

Insert

YFP

Use

Broad-host range

Tags

Expression

Bacterial

Mutation

Promoter

Ptac

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pDEST mfap4:tdTomato

Plasmid

#135214

Purpose

Red tagged macrophages

Depositor

David Tobin

Article

Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

Insert

mfap4 promoter and tdTomato

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Available since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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