We narrowed to 2,449 results for: PORA

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APEX2-SOPP3-TM

Plasmid

#229077

Purpose

Expresses APEX2-SOPP3 in the cell surface

Depositor

Weijun Pan

Article

Qu et al Cell Res. 2025 Feb;35(2):149-152. doi: 1

Insert

Express APEX2-SOPP3 in the cell surface (APEX2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sACt-mCherry-NES

Plasmid

#138215

Purpose

Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; cytosol targeted.

Depositor

Jin Zhang

Article

Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

Insert

sACt-mCherry-NES (Adcy10 Rat)

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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hSyn-Aion-citrine

Plasmid

#192569

Purpose

AAV-vector for optogenetic long-term silencing of neurons

Depositor

Simon Wiegert

Article

Rodriguez-Rozada et al Commun Biol. 2022 Jul 9;5(1):687. doi: 1

Insert

Aion

Use

AAV

Tags

Citrine

Expression

Mammalian

Mutation

T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…

Promoter

human synapsin

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pSF3-Flag-Cbir-Cdc25C WT_Myc-Nbir-14-3-3e

Plasmid

#90010

Purpose

Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Cdc25C and N-terminally tagged NBir[2-256]-14-3-3e

Depositor

Julien Béthune

Article

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

CBirA*-Cdc25C WT
NBirA*-14-3-3e

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Y1214F-VEGFR2-mCherry

Plasmid

#139609

Purpose

Expresses a mutant (Y1214F) VEGFR2-mCherry fusion protein in mammalian cells

Depositor

Michael Welsh

Article

Pietila et al Cells. 2019 Dec 15;8(12). pii: cells8121

Insert

KDR (KDR Human)

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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eSpCas9-EF-5Nanog_2

Plasmid

#174871

Purpose

CRISPR vector for generating Nanog STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

sgRNA for mouse Nanog (Nanog Synthetic)

Use

CRISPR

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-ChroME-ST-P2A-H2B-mRuby3

Plasmid

#170161

Purpose

Cation channelrhodopsin ChroME targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

Depositor

Hillel Adesnik

Article

Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

Insert

ChroME-P2A-H2B-mRuby3

Use

AAV

Expression

Mammalian

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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hSyn-Aion-ts-citrine-ER

Plasmid

#192575

Purpose

AAV-vector for optogenetic long-term silencing of neurons

Depositor

Simon Wiegert

Article

Rodriguez-Rozada et al Commun Biol. 2022 Jul 9;5(1):687. doi: 1

Insert

Aion

Use

AAV

Tags

ts-Citrine-ER

Expression

Mammalian

Mutation

T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…

Promoter

human synapsin

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pX330-NL-DHFR-SpCas9

Plasmid

#124522

Purpose

Expresses SpCas9 fused to DHFR domains on both the N-terminus and an internal loop in mammalian cells

Depositor

Amit Choudhary

Article

Manna et al Angew Chem Int Ed Engl. 2019 May 6;58(19

Insert

NL-DHFR-SpCas9

Use

AAV

Tags

Destabilized domain of E.coli dihydrofolate reduc…

Expression

Mammalian

Promoter

Cbh

Available Since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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eSpCas9-EF-5Pou5f1

Plasmid

#174872

Purpose

CRISPR vector for generating Pou5f1 STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

sgRNA for mouse Pou5f1 (Pou5f1 Synthetic)

Use

CRISPR

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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PDZb-beta2(616-951)-mCherry

Plasmid

#100736

Purpose

To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

AP2B1 (AP2B1 Human)

Tags

PDZb and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pAL130

Plasmid

#22475

Purpose

Expresses N terminal domain of PIF3 fused to mYFP, for red-light-induced binding to Phy

Depositor

Christopher Voigt

Article

Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

Insert

PIF3NT (PIF3 Mouse, Mustard Weed)

Use

Synthetic Biology

Tags

mYFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pCK314

Plasmid

#110545

Purpose

Modified (delta CRP-binding site) rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites. Allows precise & sustained gene expression in cyanobacteria

Depositor

John Heap

Article

Kelly et al ACS Synth Biol. 2018 Apr 2. doi: 10.1021

Inserts

Modified PrhaBAD (CRP-binding sites removed)
yfp
rhaS

Expression

Bacterial

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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FastLightR-bRaf-mVenus

Plasmid

#162155

Purpose

Expresses FastLightR-bRaf-mVenus in mammalian cells

Depositor

Andrei Karginov

Article

Shaaya et al Elife. 2020 Sep 23;9. pii: 60647. doi: 1

Insert

FastLightR-bRaf-mVenus

Tags

mVenus

Expression

Mammalian

Mutation

Valine 600 is substituted with Glutamic acid

Promoter

CMV

Available Since

Dec. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-GRAB_r5-HT1.0

Plasmid

#208725

Purpose

Expresses the red 5-HT sensor GRAB_r5-HT1.0 in neurons in the presence of Cre recombinase

Depositor

Yulong Li

Article

Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

Insert

Red fluorescent 5-HT sensor GRAB_r5-HT1.0

Use

AAV

Promoter

EF1a

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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mcherry Gamma 9 ---pcDNA3.1

Plasmid

#64203

Purpose

G protein Gamma cone(9) subunit tagged with mcherry on the N terminus

Depositor

Narasimhan Gautam

Article

Karunarathne et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

Insert

G protein Gamma 9 (GNGT2 Human)

Tags

6xHis and mcherry

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only
pSF3-Flag-Cbir-Ago2_Myc-Nbir-TNRC6C

Plasmid

#90014

Purpose

Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-TNRC6C

Depositor

Julien Béthune

Article

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

CBirA*-Ago2
NBirA*-TNRC6C

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pBEST-p70a-UTR1-NTerm 6xHis-zmADH-T500

Plasmid

#92228

Purpose

Expression of zmADH using the p70a promoter and UTR1

Depositor

Lital Alfonta

Article

Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

Insert

NTerm 6xHis Tagged zmADH

Use

Synthetic Biology; Cell free protein synthesis (c…

Tags

6xHis

Expression

Bacterial

Promoter

p70a

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-GRAB_g5-HT3.0mut

Plasmid

#208724

Purpose

Expresses the green 5-HT sensor GRAB_g5-HT3.0mut in neurons in the presence of Cre recombinase

Depositor

Yulong Li

Article

Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

Insert

Green fluorescent 5-HT sensor GRAB_g5-HT3.0mut

Use

AAV

Promoter

EF1a

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-pmEMBer

Plasmid

#174441

Purpose

Plasma membrane-targeted ERK monobody binder for local inhibition of ERK activity in live cells.

Depositor

Jin Zhang

Article

Keyes et al eLife. 2020 May 26;9. pii: 57410. doi: 1

Insert

pmEMBer

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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