We narrowed to 5,173 results for: pcDNA3.1+

1. XE256 pCDNA3.1(zeo)-hDsh3-deltaCTER

   Plasmid

   #16753

   Depositor

   Randall Moon

   Article

   Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

   Insert

   FLAG-hDsh3-deltaCTER (DVL3 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   delta CTER

   Promoter

   Available since

   March 14, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1/NDUFS2 _34-78/myc-His

   Plasmid

   #127496

   Purpose

   Expresses myc-tagged _34-78 NDUFS2 in mammalian cells

   Depositor

   Shashi Jain, Dieter Wolf

   Article

   Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

   Insert

   NDUFS2 _34-78 (NDUFS2 Human)
   

   Use

   Tags

   myc-His

   Expression

   Mammalian

   Mutation

   NDUFS2 _34-78

   Promoter

   CMV

   Available since

   July 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. Human NCKX4-AS-short-FlagTag (pcDNA3.1+)

   Plasmid

   #75212

   Purpose

   Mammalian expression of SLC24A4

   Depositor

   Jonathan Lytton

   Article

   Li et al J Biol Chem. 2002 Dec 13;277(50):48410-7

   Insert

   SLC24A4 (SLC24A4 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   alternatively spliced exon absent

   Promoter

   CMV

   Available since

   June 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(+)mGAT1-577-CFP-578CT

   Plasmid

   #41682

   Depositor

   Henry Lester

   Article

   Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

   Insert

   Mus musculus GABA transporter 1 (Slc6a1 Synthetic, Mouse)
   

   Use

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   “Monomerizing” CFP A206K mutation; CFP inserted d…

   Promoter

   CMV

   Available since

   April 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1(+)mGAT1-577-YFP-578CT

   Plasmid

   #41681

   Depositor

   Henry Lester

   Article

   Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

   Insert

   Mus musculus GABA transporter 1 (Slc6a1 Synthetic, Mouse)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   “Monomerizing” YFP A206K mutation; YFP inserted d…

   Promoter

   CMV

   Available since

   April 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. Human NCKX2-AS-short-FlagTag (pcDNA3.1+)

   Plasmid

   #75202

   Purpose

   Mammalian expression of SLC24A2

   Depositor

   Jonathan Lytton

   Article

   Visser et al J Biol Chem. 2007 Feb 16;282(7):4453-62.

   Insert

   SLC24A2 (SLC24A2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   alternatively spliced exon absent

   Promoter

   CMV

   Available since

   June 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-FLAG-SBP-STAG2 (S1215X)

   Plasmid

   #73970

   Purpose

   STAG2 cDNA (CCDS43990) S1215X

   Depositor

   Todd Waldman

   Article

   Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

   Insert

   STAG2 (STAG2 Human)
   

   Use

   Tags

   FLAG-SBP

   Expression

   Mammalian

   Mutation

   S1215X

   Promoter

   Available since

   March 14, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3.1-FLAG-SBP-STAG2 (Q1117X)

   Plasmid

   #73968

   Purpose

   STAG2 cDNA (CCDS43990) Q1117X

   Depositor

   Todd Waldman

   Article

   Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

   Insert

   STAG2 (STAG2 Human)
   

   Use

   Tags

   FLAG-SBP

   Expression

   Mammalian

   Mutation

   Q1117X

   Promoter

   Available since

   March 14, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3.1-FLAG-SBP-STAG2 (S1075X)

   Plasmid

   #73967

   Purpose

   STAG2 cDNA (CCDS43990) S1075X

   Depositor

   Todd Waldman

   Article

   Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

   Insert

   STAG2 (STAG2 Human)
   

   Use

   Tags

   FLAG-SBP

   Expression

   Mammalian

   Mutation

   S1075X

   Promoter

   Available since

   March 14, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3.1-FLAG-SBP-STAG2 (S653X)

    Plasmid

    #73965

    Purpose

    STAG2 cDNA (CCDS43990) S653X

    Depositor

    Todd Waldman

    Article

    Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

    Insert

    STAG2 (STAG2 Human)
    

    Use

    Tags

    FLAG-SBP

    Expression

    Mammalian

    Mutation

    S653X

    Promoter

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3.1 2xFLAG-2xSTREP-Emsy (mouse)

    Plasmid

    #236419

    Purpose

    transient overexpression of EMSY in mammalian cells

    Depositor

    Michele Pagano

    Article

    Marzio et al Cell. 2022 Jan 6;185(1):169-183.e19. doi

    Insert

    EMSY (2210018M11Rik Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1 2xFLAG-2xSTREP-EMSY (human)

    Plasmid

    #236420

    Purpose

    transient overexpression of EMSY in mammalian cells

    Depositor

    Michele Pagano

    Article

    Marzio et al Cell. 2022 Jan 6;185(1):169-183.e19. doi

    Insert

    EMSY (EMSY Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1 2xFLAG-2xSTREP-Keap1 (mouse)

    Plasmid

    #236421

    Purpose

    transient overexpression of KEAP1 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Marzio et al Cell. 2022 Jan 6;185(1):169-183.e19. doi

    Insert

    KEAP1 (Keap1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1 2xFLAG-2xSTREP-KEAP1 (human)

    Plasmid

    #236422

    Purpose

    transient overexpression of KEAP1 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Marzio et al Cell. 2022 Jan 6;185(1):169-183.e19. doi

    Insert

    KEAP1 (KEAP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMJ1.19 (AAVS1 RNA donor in pCDNA3.1)

    Plasmid

    #238007

    Purpose

    300bp donor RNA complementary to Cas9 cut site at AAVS1 (Addgene plasmid #72833) with intronic sequence intervening the 3bp insertion signature (ATC). Can be used to measure RT-DSBR in human cells.

    Depositor

    Simon Powell, Agnel Sfeir

    Article

    Jalan et al Nat Commun. 2025 May 10;16(1):4349. doi:

    Insert

    AAVS1 (AAVS1, PPP1R12C Human)
    

    Use

    Rna donor to study rt-dsbr

    Tags

    Expression

    Mammalian

    Mutation

    3bp insertion signature (ATC) at Cas9 cut site in…

    Promoter

    CMV

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3-lk-HiBiT

    Plasmid

    #233064

    Purpose

    Expresses mouse ADGRL3 (UniProt Q80TS3-3). An N-terminal AFLA-tag (SRLEEELRRRLTE) and HiBiT tag (VSGWRLFKKIS) follow the endogenous ADGRL3 signal peptide. Also includes a C-terminal HiBiT tag.

    Depositor

    Jonathan Javitch

    Article

    Perry-Hauser et al J Biol Chem. 2025 Jan 9:108174. doi: 10.

    Insert

    ADGRL3 (Adgrl3 Mouse)
    

    Use

    Tags

    ALFA and HiBiT

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3-T923G

    Plasmid

    #233063

    Purpose

    Expresses cleavage-deficient mouse ADGRL3-T923G (UniProt Q80TS3-3). An N-terminal AFLA-tag (SRLEEELRRRLTE) and HiBiT tag (VSGWRLFKKIS) follow the endogenous ADGRL3 signal peptide.

    Depositor

    Jonathan Javitch

    Article

    Perry-Hauser et al J Biol Chem. 2025 Jan 9:108174. doi: 10.

    Insert

    ADGRL3 (Adgrl3 Mouse)
    

    Use

    Tags

    ALFA and HiBiT

    Expression

    Mammalian

    Mutation

    Threonine 923 to Glycine

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1 Cas9-P2A-dTomato-PS9

    Plasmid

    #231911

    Purpose

    For packaging spCas9, dTomato mRNA into SARS-CoV-2 virus-like particles (VLPs) with a 'PS9' packaging signal

    Depositor

    Sriram Neelamegham

    Article

    Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

    Insert

    Cas9-P2A-dTomato
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1 Cas9-P2A-dTomato-T20

    Plasmid

    #231912

    Purpose

    For packaging spCas9, dTomato mRNA into SARS-CoV-2 virus-like particles (VLPs) with a 'T20' packaging signal

    Depositor

    Sriram Neelamegham

    Article

    Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

    Insert

    Cas9-P2A-dTomato
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1 T21R-Nb139-T2A-mCherry

    Plasmid

    #225589

    Purpose

    Mammalian expression vector for TRIM21 RING-Nb139 anti-p53 degrader with a self-cleaving T2A-mCherry fluorescent expression reporter

    Depositor

    William McEwan

    Article

    Benn et al Science. 2024 Aug 30;385(6712):1009-1016

    Insert

    T21R-Nb139-T2A-mCherry
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3.1-myc:Skp1(dC111):gs10:FRB:HA

    Plasmid

    #228532

    Purpose

    A fragment of human Skp1 protein in fusion with FRB domain through a flexible glycine-serine linker.

    Depositor

    Roman Jerala

    Article

    Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80

    Insert

    myc:Skp1(dC111):gs10:FRB:HA (SKP1 Synthetic, Human)
    

    Use

    Tags

    HA and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3.1-HA-GTSE1-S91, 262 ,724A

    Plasmid

    #224737

    Purpose

    For expression of HA-tagged GTSE1 protein with S91, 262 ,724A mutation

    Depositor

    Michele Pagano

    Article

    García-Vázquez et al bioRxiv 2024.06.26.600797

    Insert

    GTSE1 (GTSE1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Serine 91, 262 ,724 to Alanine

    Promoter

    CMV

    Available since

    Dec. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3.1-SLC25A46-P2A-Electra1 (pAH9)

    Plasmid

    #227614

    Purpose

    Overexpression of SLC25A46 in mammalian cell lines

    Depositor

    Luke Chao

    Article

    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

    Insert

    SLC25A46 (SLC25A46 Human)
    

    Use

    Tags

    P2A-mElectra1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.1-SLC25A46[∆2-83]-P2A-Electra1 (pAH14)

    Plasmid

    #227619

    Purpose

    Overexpression of SLC25A46 lacking its N-terminal region in mammalian cell lines

    Depositor

    Luke Chao

    Article

    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

    Insert

    SLC25A46 (SLC25A46 Human)
    

    Use

    Tags

    P2A-mElectra1

    Expression

    Mammalian

    Mutation

    deleted aa 2-83

    Promoter

    CMV, T7

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3.1-CMV-SV40NLS-OgeuIscBE193A-3XHA

    Plasmid

    #222861

    Purpose

    This plasmid codes for the OgeuIscB nickase

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Insert

    OgeuIscB Nickase
    

    Use

    CRISPR

    Tags

    3X HA and Nucleoplasmin NLS and ITR2

    Expression

    Mammalian

    Mutation

    E193A for nickase mutation

    Promoter

    CMV

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.1-myc-GFP-TNRC6A-SD-CIM1-WT

    Plasmid

    #215898

    Purpose

    Expression of TNRC6A-CIM1 domain

    Depositor

    Kumiko Ui-Tei

    Article

    Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    myc-GFP

    Expression

    Mammalian

    Mutation

    contain aa1224-1528

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3.1-myc-GFP-TNRC6A-SD-CIM2-WT

    Plasmid

    #215899

    Purpose

    Expression of TNRC6A-CIM2 domain

    Depositor

    Kumiko Ui-Tei

    Article

    Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    myc-GFP

    Expression

    Mammalian

    Mutation

    contain aa1529-1709

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.1(+) EGFP-Rac1(G12C, K96H)

    Plasmid

    #224281

    Purpose

    Cell transfection and expression of Rac1 (G12C, K96H)

    Depositor

    Kevan Shokat

    Article

    Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

    Insert

    Rac1 (RAC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G12C, K96H

    Promoter

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3.1(+) EGFP-Rap1A(G12C, L96F)

    Plasmid

    #224277

    Purpose

    Cell transfection and expression of Rap1A (G12C, L96F)

    Depositor

    Kevan Shokat

    Article

    Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

    Insert

    Rap1A (RAP1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G12C, L96F

    Promoter

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3.1-Myc-BirA*-RNF8-C403S

    Plasmid

    #221685

    Purpose

    Mammalian expression of Myc-tagged BirA*-RNF8 (C403S) fusion protein for BioID

    Depositor

    Derrick Sek Tong Ong

    Article

    Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

    Insert

    RNF8 (RNF8 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C403S

    Promoter

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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