We narrowed to 9,389 results for: Coli

1. pBSMrnaSeph

   Plasmid

   #67507

   Purpose

   Express the tRNAMet scaffold with the sephadex aptamer tag

   Depositor

   Luc Ponchon

   Article

   Ponchon et al Nat Protoc. 2009;4(6):947-59. doi: 10.10

   Insert

   modified tRNAMet with sephadex aptamer
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lpp

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pDEST-periHisMBP

   Plasmid

   #11086

   Depositor

   David Waugh

   Article

   Nallamsetty et al Protein Sci. 2005 Dec . 14(12):2964-71.

   Type

   Empty backbone

   Use

   Tags

   6xHis, MBP, and MBP signal peptide

   Expression

   Bacterial

   Mutation

   Promoter

   Tac (lactose/IPTG inducible)

   Available since

   Feb. 14, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. ALPaGA

   Plasmid

   #175272

   Purpose

   Lactate biosensor in anaerobic conditions

   Depositor

   Jerome Bonnet

   Article

   Zuniga et al ACS Synth Biol. 2021 Dec 1. doi: 10.1021

   Insert

   LldR_Synthetic_promoter_sfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pST50Trc1-HISNDHFR

   Plasmid

   #63940

   Purpose

   Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

   Depositor

   Song Tan

   Article

   Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

   Type

   Empty backbone

   Use

   Tags

   6xHis; TEV cleavable

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PT7lacO-RF1-His6

   Plasmid

   #224372

   Purpose

   Express RF1 protein for his-tagged purification

   Depositor

   Richard Murray

   Article

   Zhang et al bioRxiv 2024.06.19.599772

   Insert

   RF1 (prfA E. coli)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pST50Trc3-HISNDHFR

   Plasmid

   #63976

   Purpose

   Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

   Depositor

   Song Tan

   Article

   Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

   Type

   Empty backbone

   Use

   Tags

   6xHis; TEV cleavable

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pST50Trc4-HISNDHFR

   Plasmid

   #63994

   Purpose

   Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

   Depositor

   Song Tan

   Article

   Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

   Type

   Empty backbone

   Use

   Tags

   6xHis; TEV cleavable

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pST50Trc2-HISNDHFR

   Plasmid

   #63958

   Purpose

   Position 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

   Depositor

   Song Tan

   Article

   Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

   Type

   Empty backbone

   Use

   Tags

   6xHis; TEV cleavable

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAC574

   Plasmid

   #127975

   Purpose

   AMA1 plasmid with hygB selection marker, and USER cassette (PacI/Nt.BbvCI)

   Depositor

   Uffe Mortensen

   Article

   Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

   Insert

   hygB (hygromycin resistance marker)
   

   Use

   Fungal expression

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Aspergillus nidulans trpC promoter

   Available since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. SJZ5-HPH

    Plasmid

    #228258

    Purpose

    Template plasmid for yeast gene knockout, confers antibiotic resistance to hygromycin B

    Depositor

    Zhiping Xie

    Article

    Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

    Insert

    hph
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SSA1 S.c.

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTKDP-neo

    Plasmid

    #71320

    Purpose

    Donor plasmid - kanamycin resistant donor cassette

    Depositor

    Thomas Kuhlman

    Article

    Tas et al PLoS One. 2015 Sep 2;10(9):e0136963. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTKDP-dhfr

    Plasmid

    #71323

    Purpose

    Donor plasmid - trimethoprim resistant donor cassette

    Depositor

    Thomas Kuhlman

    Article

    Tas et al PLoS One. 2015 Sep 2;10(9):e0136963. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pKL11-GCaMP6f bb100

    Plasmid

    #158983

    Purpose

    Constitutive expression (sigma 70 promoter) of GCaMP6f fluorophore in E. coli.

    Depositor

    Joel Kralj

    Article

    Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

    Insert

    GCaMP6f
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    codon optimized for e. coli from Addgene#100833

    Promoter

    Bba_J23100 (sigma 70)

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAN3938

    Plasmid

    #74697

    Purpose

    Circuit plasmid: 0xF6

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

    Insert

    Circuit 0xF6
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSEVA3b61

    Plasmid

    #58310

    Purpose

    Shuttle vector: pUC ori, pTHT15 ori for use in E. coli/B. subtilis. Cannot modify orientation of cat gene; promoter too weak in E. coli, probably transcribed via repB promoter in bicistronic manner.

    Depositor

    Tom Ellis

    Article

    Wright et al ACS Synth Biol. 2014 May 13.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pzntA_LacZ

    Plasmid

    #216325

    Purpose

    PZntA promoter driving expression of lacZ

    Depositor

    Mark Styczynski

    Article

    McNerney et al Sci Adv. 2019 Sep 25;5(9):eaax4473. doi:

    Insert

    b-Galactosidase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pT7_zntR

    Plasmid

    #216324

    Purpose

    T7 promoter driving expression of zntR

    Depositor

    Mark Styczynski

    Article

    McNerney et al Sci Adv. 2019 Sep 25;5(9):eaax4473. doi:

    Insert

    Zinc-responsive activator
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pTAKN2_J23119-sgRNA

    Plasmid

    #108552

    Purpose

    Bacterial sgRNA expression vector

    Depositor

    Akihiko Kondo

    Article

    Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

    Insert

    2x Streptococcus pyogenes sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHV738

    Plasmid

    #117476

    Purpose

    Expression of NMT1

    Depositor

    Richard Kahn

    Article

    Van Valkenburgh et al Methods Enzymol. 2002;344:186-93.

    Insert

    NMT1 (NMT1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDisplay-AP-CFP-TM

    Plasmid

    #20861

    Depositor

    Alice Ting

    Article

    Howarth et al Proc Natl Acad Sci U S A. 2005 May 24. 1

    Insert

    AP-CFP-TM
    

    Use

    Tags

    CFP, HA, and myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pPL5071_TEF1*-Cre_URA3

    Plasmid

    #60930

    Purpose

    Constitutive expression vector for Cre-recombinase containing URA3 selection marker.

    Depositor

    Robert Piper

    Article

    MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

    Insert

    Cre
    

    Use

    Tags

    Driven from a mutant TEF1 promoter with 0.16x act…

    Expression

    Yeast

    Mutation

    Promoter

    mutant TEF1

    Available since

    Feb. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAC5.1-bgal-V5His

    Plasmid

    #136238

    Purpose

    Protein expression of Beta-galactosidase with C-terminal V5 and His tags

    Depositor

    Steven Reppert, Aziz Sancar

    Article

    Zhu et al Curr Biol. 2005 Dec 6;15(23):R953-4. doi

    Insert

    Beta-galactosidase
    

    Use

    Tags

    His and V5

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pJPC210

    Plasmid

    #234532

    Purpose

    Int13 Recombinase switch; plant T-DNA

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Physiol. 2025 Mar 1;197(3):kiaf073

    Insert

    RUBY
    

    Use

    Tags

    Expression

    Plant

    Mutation

    none

    Promoter

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pJPC430

    Plasmid

    #234529

    Purpose

    CinH Recombinase switch; plant T-DNA

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Physiol. 2025 Mar 1;197(3):kiaf073

    Insert

    RUBY
    

    Use

    Tags

    Expression

    Plant

    Mutation

    none

    Promoter

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV-FLEX-tTA

    Plasmid

    #149363

    Purpose

    AAV-mediated and Cre-dependent expression of tTA under the CAG promoter.

    Depositor

    Takeshi Imai

    Article

    Leiwe et al bioRxiv 2022.10.20.512984

    Insert

    tTA2
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pPL5606_TEF1*-Cre_ADE2

    Plasmid

    #60932

    Purpose

    Constitutive expression vector for Cre-recombinase containing ADE2 selection marker.

    Depositor

    Robert Piper

    Article

    MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

    Insert

    Cre
    

    Use

    Tags

    Driven from a mutant TEF1 promoter with 0.16x act…

    Expression

    Yeast

    Mutation

    Promoter

    mutant TEF1

    Available since

    Feb. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBSK

    Plasmid

    #67504

    Purpose

    Express the tRNALys scaffold

    Depositor

    Luc Ponchon

    Article

    Ponchon et al Nat Protoc. 2009;4(6):947-59. doi: 10.10

    Insert

    modified tRNALys human
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lpp

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

28. pTW357b

    Plasmid

    #227930

    Purpose

    Low-stringency accessory plasmid for selection

    Depositor

    Tina Wang

    Article

    Yang et al Nat Chem Biol. 2025 Jan 13. doi: 10.1038

    Inserts

    gIII (recoded)
    T7c
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    R632S

    Promoter

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGK401red-­rtetTAK8N, L131L

    Plasmid

    #165968

    Purpose

    Expresses rTetTA (K8N, L131L) mutant

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

    Insert

    tetR
    

    Use

    Synthetic Biology

    Tags

    three copies of VP16 (VP48)

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. PT7lacO-His6-IF2

    Plasmid

    #224367

    Purpose

    Express IF2 protein for his-tagged purification

    Depositor

    Richard Murray

    Article

    Zhang et al bioRxiv 2024.06.19.599772

    Insert

    IF2 (infB E. coli)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. PT7lacO-RF3-His6

    Plasmid

    #224373

    Purpose

    Express RF3 protein for his-tagged purification

    Depositor

    Richard Murray

    Article

    Zhang et al bioRxiv 2024.06.19.599772

    Insert

    RF3 (prfB E. coli)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. PT7lacO-His6-AsnRS

    Plasmid

    #224363

    Purpose

    Express AsnRS protein for his-tagged purification

    Depositor

    Richard Murray

    Article

    Zhang et al bioRxiv 2024.06.19.599772

    Insert

    AsnRS (asnS E. coli)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. PT7lacO-RRF-His6

    Plasmid

    #224374

    Purpose

    Express RRF protein for his-tagged purification

    Depositor

    Richard Murray

    Article

    Zhang et al bioRxiv 2024.06.19.599772

    Insert

    RRF (frr E. coli)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. PT7lacO-His6-IF3

    Plasmid

    #224368

    Purpose

    Express IF3 protein for his-tagged purification

    Depositor

    Richard Murray

    Article

    Zhang et al bioRxiv 2024.06.19.599772

    Insert

    IF3 (infC E. coli)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. PT7lacO-His6-IF1

    Plasmid

    #224366

    Purpose

    Express IF1 protein for his-tagged purification

    Depositor

    Richard Murray

    Article

    Zhang et al bioRxiv 2024.06.19.599772

    Insert

    IF1 (infA E. coli)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. PT7lacO-His6-ThrRS

    Plasmid

    #224365

    Purpose

    Express ThrRS protein for his-tagged purification

    Depositor

    Richard Murray

    Article

    Zhang et al bioRxiv 2024.06.19.599772

    Insert

    ThrRS (thrS E. coli)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. PT7lacO-His6-NDK

    Plasmid

    #224376

    Purpose

    Express NDK protein for his-tagged purification

    Depositor

    Richard Murray

    Article

    Zhang et al bioRxiv 2024.06.19.599772

    Insert

    NDK (ndk E. coli)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pJLG039

    Plasmid

    #192982

    Purpose

    BHR plasmid (IncP ori) expressing T7 polymerase under control of the lac promoter

    Depositor

    Clay Fuqua

    Article

    Greenwich et al Microbiol Resour Announc. 2023 Feb 16;12

    Insert

    T7 polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pPL5608_TEF1*-Cre_TRP1

    Plasmid

    #60931

    Purpose

    Constitutive expression vector for Cre-recombinase containing TRP1 selection marker.

    Depositor

    Robert Piper

    Article

    MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

    Insert

    Cre
    

    Use

    Tags

    Driven from a mutant TEF1 promoter with 0.16x act…

    Expression

    Yeast

    Mutation

    Promoter

    Mutant TEF1

    Available since

    Feb. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pDR-GW FLII12Pglu-700μδ6

    Plasmid

    #28002

    Depositor

    Wolf Frommer

    Article

    Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

    Insert

    FLII12Pglu-700μδ6
    

    Use

    Tags

    eCFP and eYFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only