We narrowed to 13,482 results for: cas9 genes

1. pRS315e_pGal-dCas9-PmCDA1

   Plasmid

   #79615

   Purpose

   Expresses dCas9-PmCDA1 in yeast cells

   Depositor

   Akihiko Kondo

   Article

   Nishida et al Science. 2016 Aug 4. pii: aaf8729.

   Insert

   SpCas9
   

   Use

   Tags

   PmCDA1

   Expression

   Yeast

   Mutation

   D10A and H840A for nuclease deficient Cas9

   Promoter

   pGal1

   Available since

   July 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. SpRY dCas9 KRAB

   Plasmid

   #188501

   Purpose

   Expresses FLAG tagged SpRY dCas9 KRAB

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A/A61R/H840A/L1111R/D1135L/S1136W/G1218K/E1219…

   Promoter

   EF1a

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. LentiCas9-sgVRK1 #1-Blast

   Plasmid

   #199646

   Purpose

   Expresses Cas9 and sgRNA guide targeting VRK1

   Depositor

   William Hahn

   Article

   So et al JCI Insight. 2022 Oct 10;7(19):e158755.

   Insert

   N/A (VRK1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIVT-dCas9-mCherry

   Plasmid

   #174443

   Purpose

   in vitro transcription template for dCas9-mCherry

   Depositor

   Michael Lampson

   Article

   Kumon et al Cell. 2021 Sep 16;184(19):4904-4918.e11.

   Insert

   Cas9 Endonuclease Dead
   

   Use

   In vitro transcription template

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   T7 SP6

   Available since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. xCas9(3.6)-BE4

   Plasmid

   #108386

   Purpose

   Mammalian expression vector for xCas9(3.6)-BE4

   Depositor

   David Liu

   Article

   Hu et al Nature. 2018 Feb 28. pii: nature26155. d

   Insert

   xCas9(3.6)-BE4
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

   Promoter

   Available since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-Cas9-NRRH

   Plasmid

   #136924

   Purpose

   Expresses Cas9-NRRH in mammalian cells

   Depositor

   David Liu

   Article

   Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

   Insert

   Cas9-NRRH
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   see manuscript

   Promoter

   CMV

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-Cas9-NRTH

   Plasmid

   #136925

   Purpose

   Expresses Cas9-NRTH in mammalian cells

   Depositor

   David Liu

   Article

   Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

   Insert

   Cas9-NRTH
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   see manuscript

   Promoter

   CMV

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. xCas9(3.6)-BE3

   Plasmid

   #108385

   Purpose

   Mammalian expression vector for xCas9(3.6)-BE3

   Depositor

   David Liu

   Article

   Hu et al Nature. 2018 Feb 28. pii: nature26155. d

   Insert

   xCas9(3.6)-BE3
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

   Promoter

   Available since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNHEJ-Cas9-recX

   Plasmid

   #158715

   Purpose

   CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis. Helper plasmid expresses Cas9, NHEJ machinery of M. marinum and recX

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

   Insert

   cas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   TetO

   Available since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSpCas9(BB)-2A-GFP-G3BP1(2)

    Plasmid

    #136059

    Purpose

    G3BP1 gRNA (#2) inserted into the pSpCas9(BB)-2A-GFP plasmid (GTATTACACACTGCTGAACC)

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    G3BP1 (G3BP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330 LC-FKBP-Cas9

    Plasmid

    #162724

    Purpose

    Mammalian expression

    Depositor

    Amit Choudhary

    Article

    Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

    Insert

    FKBP F36V
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. CBh-dCas9-Mll3SET

    Plasmid

    #108452

    Purpose

    Expression vector of CRISPR/dCas9 fused with SET domain of mouse MLL3 gene, driven by CBh promoter

    Depositor

    Bing Ren

    Article

    Yan et al Cell Res. 2018 Jan 9. pii: cr20181. doi:

    Insert

    dCas9, MLL3 (Kmt2c Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHAGE-TO-Sp dCas9-mVenusN

    Plasmid

    #191396

    Purpose

    SpdCas9 fused to N-terminal part of mVenus

    Depositor

    Albert Jeltsch

    Article

    Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

    Insert

    N-terminal part of split mVenus
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330_TOMM20 sgRNA / hSpCas9

    Plasmid

    #172836

    Purpose

    Mammalian expression of a sgRNA targeting the intron 4 (last intron) of TOMM20 (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    sgRNA targeting intron 4 of TOMM20 under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. p221z-dCAS9p-t35s

    Plasmid

    #118387

    Purpose

    Entry clone containing dCAS9p and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway system

    Depositor

    Ari Pekka Mähönen

    Article

    Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

    Insert

    dCAS9p
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pnCas9-miniSdd6-PBE

    Plasmid

    #204858

    Purpose

    For cytosine base editing using miniSdd6 in monocotyledon plants (transient transformation)

    Depositor

    Caixia Gao

    Article

    Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

    Insert

    miniSdd6-nSpCas9(D10A)-UGI
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A in SpCas9

    Promoter

    ZmUbi-1

    Available since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSpCas9(BB)-2A-GFP-G3BP1(1)

    Plasmid

    #136058

    Purpose

    G3BP1 gRNA (#1) inserted into the pSpCas9(BB)-2A-GFP plasmid (GTAGTCCCCTGCTGGTCGGGC)

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    G3BP1 (G3BP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. dCas9-CRY2Clust-FUSN

    Plasmid

    #200967

    Purpose

    induction of phase-separated condensate mediated by FUSN-IDR

    Depositor

    Jian Xu

    Article

    Kim et al Sci Adv. 2023 Mar 31;9(13):eadg1123. doi

    Insert

    FUSN
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti6.3-efSaCas9-BlastR

    Plasmid

    #220971

    Purpose

    Expresses human codon-optimized efSaCas9

    Depositor

    Hyongbum Kim

    Article

    Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

    Insert

    efSaCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. TetO-dCas9-mD3A

    Plasmid

    #78255

    Purpose

    Expresses dead Cas9 (dCas9) fused to inactive DNMT3A catalytic domain (CD) linked to IRES-mCherry under the control of TetOp and a minimal CMV promoter

    Depositor

    Grant Challen

    Article

    McDonald et al Biol Open. 2016 May 11. pii: bio.019067.

    Insert

    DNMT3A CD aa 598-912 (DNMT3A Human)
    

    Use

    CRISPR

    Tags

    Flag epitope tag

    Expression

    Mammalian

    Mutation

    E756A

    Promoter

    CMV

    Available since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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