We narrowed to 23,974 results for: nes

1. pCas9-CAT

   Plasmid

   #80323

   Purpose

   Encodes Cas9 and chloramphenicol acetyltransferase (CAT)

   Depositor

   Sebastian Lourido

   Article

   Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

   Inserts

   Cas9
   Chloramphenicol acetyltransferase
   

   Use

   CRISPR

   Tags

   3X FLAG and NLS

   Expression

   Mutation

   Promoter

   TgTUB1

   Available since

   Sept. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA GAL4 CREB 1-341 M1

   Plasmid

   #22973

   Depositor

   Marc Montminy

   Article

   Conkright et al Mol Cell. 2003 Apr . 11(4):1101-8.

   Insert

   CREB (Creb1 GAL4-fusion, Rat)
   

   Use

   Tags

   GAL4 DNA-binding domain

   Expression

   Mammalian

   Mutation

   Changed Serine 133 to Alanine of CREB

   Promoter

   Available since

   Feb. 4, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGG-PIP56

   Plasmid

   #196794

   Purpose

   Contains PR1 promoter

   Depositor

   Marc Somssich

   Article

   Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

   Insert

   PATHOGENESIS-RELATED GENE 1 (PR1 Mustard Weed)
   

   Use

   Greengate (gg) entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAc-y1sgRNA-Cas9

   Plasmid

   #49331

   Purpose

   Expresses sgRNA targeting yellow gene and Cas9-Puro in Drosophila S2 cells

   Depositor

   Ji-Long Liu

   Article

   Bassett et al Biol Open. 2013 Dec 10. pii: bio.2013712

   Inserts

   Cas9
   dU6-sgRNA
   y1 sgRNA (y Fly)
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Insect

   Mutation

   Human codon optimised

   Promoter

   Actin-5c and Drosophila U6

   Available since

   Dec. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMT-Pav-GFP

   Plasmid

   #24286

   Depositor

   Ron Vale

   Article

   Vale et al J Cell Biol. 2009 Sep 7. 186(5):727-38.

   Insert

   Pavarotti (pav Fly)
   

   Use

   Tags

   GFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 29, 2010

   Availability

   Academic Institutions and Nonprofits only

6. GFP-DroshaS300/302A

   Plasmid

   #62530

   Purpose

   Serines 300 and 302 of Drosha are mutated to alanines

   Depositor

   Bharat Ramratnam

   Article

   Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

   Insert

   human Drosha with serines 300 and 302 changed alanines (DROSHA Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   changed serines 300 and 302 to alanines

   Promoter

   Available since

   March 20, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pTUB1-Spy.dCas9-CAT-U6-sgRNA(decoy)

   Plasmid

   #171089

   Purpose

   CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus pyogenes in Toxoplasma gondii. Suitable for generating stable cell lines.

   Depositor

   Sebastian Lourido

   Article

   Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1

   Inserts

   Decoy sgRNA
   dCas9
   Chloramphenicol acetyltransferase
   

   Use

   CRISPR; Toxoplasma gondii expression

   Tags

   3X FLAG and Nuclear Localization Signal

   Expression

   Mutation

   D10A, H840A

   Promoter

   TUB1 (Toxoplasma gondii) and U6 (Toxoplasma gondi…

   Available since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLEX307 GFP-Kif26b-C

   Plasmid

   #118483

   Purpose

   lentiviral expression of GFP-Kif26b-C in mammalian cells

   Depositor

   Henry Ho

   Article

   Karuna et al Genes (Basel). 2018 Apr 5;9(4). pii: gen

   Insert

   mouse Kif26b-C (Kif26b Mouse)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Encodes GFP fused to amino acid 1735 to 2112 of m…

   Promoter

   EF1A

   Available since

   March 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. FXYD2-luc

   Plasmid

   #31056

   Depositor

   Gerhart Ryffel

   Article

   Adalat et al J Am Soc Nephrol. 2009 May . 20(5):1123-

   Insert

   FXYD2 (FXYD2 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   FXYD2 promoter from -2382 to -1

   Promoter

   Available since

   Aug. 23, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. Lenti‐Cas9‐2A‐Blast

    Plasmid

    #73310

    Purpose

    Lentiviral vector expressing human codon-optimized S. pyogenes FLAG-tagged Cas9 and blasticidin resistance from EFS promoter

    Depositor

    Jason Moffat

    Article

    Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. EFS-SpdCas9-Dnmt3A/3L-WT

    Plasmid

    #222497

    Purpose

    Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to WT Dnmt3A/3L followed by P2A-mCherry

    Depositor

    Isaac Hilton

    Article

    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

    Insert

    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L engineered fusion
    

    Use

    Lentiviral

    Tags

    FLAG Tag

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9

    Promoter

    EFS

    Available since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. px459-AMBRA1 #3

    Plasmid

    #172606

    Purpose

    Expresses gRNA #3 against AMBRA1 and Cas9 from S. pyogenes with 2A-Puro

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    gRNA against AMBRA1 (AMBRA1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-3xFLAG-RSL1D1(WT)

    Plasmid

    #122301

    Purpose

    expresses RSL1D1 protein with a flag tag

    Depositor

    Gerardo Ferbeyre

    Article

    Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

    Insert

    RSL1D1 (RSL1D1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. px458-AMBRA1 #3

    Plasmid

    #172607

    Purpose

    Expresses gRNA #3 against AMBRA1 and Cas9 from S. pyogenes with 2A-EGFP

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    gRNA against AMBRA1 (AMBRA1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBABE-RSLID1(WT)

    Plasmid

    #122296

    Purpose

    expresses RSL1D1 protein in mammalian cells (puro resistance)

    Depositor

    Gerardo Ferbeyre

    Article

    Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

    Insert

    RSL1D1 (RSL1D1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    vector's LTR

    Available since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHEE2E-TRI

    Plasmid

    #71288

    Purpose

    Egg cell-specific promoter-controlled expression 3×FLAG-NLS-zCas9-NLS and two sgRNAs targeting three genes: ETC2, TRY, and CPC

    Depositor

    Qi-Jun Chen

    Article

    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

    Inserts

    sgRNA targeting TRY and CPC genes
    sgRNA targeting ETC2 gene
    zCas9
    

    Use

    CRISPR; Plant binary vector

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Zea mays codon-optimized Cas9

    Promoter

    EC1.2 enhancer fused to EC1.1 promoter and U6-26p…

    Available since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-FLAG-RPS14(WT)

    Plasmid

    #122297

    Purpose

    expresses RPS14 protein with a flag tag

    Depositor

    Gerardo Ferbeyre

    Article

    Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

    Insert

    RPS14 (RPS14 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. EFS-SpdCas9-Dnmt3A/3L-catΔ

    Plasmid

    #222501

    Purpose

    Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant catΔ (C706A and R832E) followed by P2A-mCherry

    Depositor

    Isaac Hilton

    Article

    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

    Insert

    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant catΔ (C706A and R832E) engineered fusion
    

    Use

    Lentiviral

    Tags

    FLAG Tag

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9; C706A and R832…

    Promoter

    EFS

    Available since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. TRE3GV-SpdCas9-Dnmt3A/3L-WT

    Plasmid

    #222502

    Purpose

    Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to WT Dnmt3A/3L and hPGK promoter driven mCherry

    Depositor

    Isaac Hilton

    Article

    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

    Inserts

    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L engineered fusion
    mCherry
    

    Use

    Lentiviral

    Tags

    FLAG Tag

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9

    Promoter

    TRE3GV and hPGK

    Available since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-Myc-CyclinD1(WT)

    Plasmid

    #122300

    Purpose

    expresses Cyclin D1 protein with a Myc tag

    Depositor

    Gerardo Ferbeyre

    Article

    Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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