We narrowed to 18,311 results for: puro

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CAG-DDdCas9VP192-T2A-EGFP-ires-puro

Plasmid

#69534

Purpose

DHFR destabilised domain (DD) fused to dCas9VP192 (S.pyogenes) on CAG expression vector. DDdCas9VP192 protein is stabilised by Trimethoprim.

Depositor

Timo Otonkoski

Article

Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

Insert

DD-dCas9VP192-T2A-EGFP

Tags

C-term T2A-EGFP and DHFR Destabilised Domain

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti c-MYC-DDK-Puro GFP

Plasmid

#123299

Purpose

Mammalian expression of GFP C-MYC-FLAG

Depositor

Giovanni Tonon

Article

Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

Insert

GFP

Use

Lentiviral

Tags

MYC-FLAG

Expression

Mammalian

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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CROPseq-EFS-HypaSpCas9-P2A-puro

Plasmid

#106965

Purpose

single-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screen

Depositor

Alex Hewitt

Insert

HypaSpCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EFS

Available Since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe-TetCMV-puro-mVenus-PA-Rac1

Plasmid

#22008

Purpose

Mammalian expression of photoactivatable Rac1

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

PA-Rac1 (RAC1 Human, Avena sativa (oat))

Use

Retroviral

Tags

mVenus

Expression

Mammalian

Mutation

LOV(Leu404-Leu546), Rac1 starts at I4 and contain…

Available Since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only
pLVXPuro-mGFP-Sec23B

Plasmid

#66598

Purpose

Expresses Sec23B as a fusion with mGFP

Depositor

David Stephens

Article

Hughes et al J Cell Sci. 2010 Dec 1;123(Pt 23):4032-8

Insert

Sec23B (SEC23B Human)

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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MelanoTag-PURO (pLJC5-GPR143-mScarlet-3xHA)

Plasmid

#160369

Purpose

Stable expression of GPR143-mScarlet-3xHA fusion protein for the isolation of melanosomes.

Depositor

David Sabatini

Article

Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

Insert

GPR143 (GPR143 Human)

Use

Lentiviral

Tags

mScarlet-3xHA

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pTet-O-ASCL1-T2A-PuroR

Plasmid

#162345

Purpose

Lentiviral expression of ASCL1 under the control of the TetON promoter

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

ASCL1 (ASCL1 Human)

Use

Lentiviral

Tags

T2A-PuroR

Expression

Mammalian

Promoter

Tet-ON

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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METTL3 shRNA 1 tet pLKO puro

Plasmid

#162985

Purpose

Tet-inducible shRNA targeting human METTL3 #1

Depositor

Mark Rubin

Article

Cotter et al Mol Cancer Res. 2021 Jun 4. pii: 1541-77

Insert

methyltransferase like 3 (METTL3 Human)

Use

Lentiviral

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-CDH5-mCitrine-IRES-puro

Plasmid

#85143

Purpose

lentiviral expression of human VE-cadherin, C-terminally tagged with mCitrine

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Insert

CDH5-mCitrine (VE-cadherin, C-terminally tagged with mCitrine (CDH5 Human)

Use

Lentiviral

Tags

mCitrine

Promoter

CAG

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-PBRM1-pgk-puro

Plasmid

#85746

Purpose

tetracyline-inducible lentiviral vector expressing human polybromo-1 (PBRM1) in mammlian cells

Depositor

Emily Dykhuizen

Article

Chowdhury et al PLoS One. 2016 Apr 21;11(4):e0153718. do

Insert

PBRM1 (PBRM1 Human)

Use

Lentiviral

Tags

none

Promoter

CMV

Available Since

March 16, 2017

Availability

Academic Institutions and Nonprofits only

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pGreenPuro-shRNA-Stx3S-C4

Plasmid

#99742

Purpose

3rd generation lentiviral vector expresses shRNA against human Stx3S with a GFP reporter.

Depositor

Thomas Weimbs

Article

Giovannone et al J Biol Chem. 2018 Apr 13;293(15):5478-54

Insert

shRNA against Stx3S

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

H1

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pQHA-USP11 WT puroR

Plasmid

#46749

Purpose

Retroviral vector that expresses wild type HA-tagged wild type human USP11

Depositor

Goedele Maertens, Gordon Peters

Article

Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

Insert

USP11 (USP11 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

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pLV-hCDC6p-Venus-puro

Plasmid

#212666

Purpose

Express Venus under CDC6 promoter

Depositor

Yumi Konagaya, Tobias Meyer

Article

Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

Insert

hCDC6p-Venus

Use

Lentiviral

Promoter

hCDC6p

Available Since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Puro-CAG-JEDI-2P

Plasmid

#179458

Purpose

Genetically encoded voltage indicator (GEVI) JEDI-2P expressed under strong mammalian promoter (CAG)

Depositor

Francois St-Pierre

Article

Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

Insert

JEDI-2P

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pWPI-IRES-Puro-Ak-TMPRSS2

Plasmid

#154986

Purpose

Lentiviral vector expressing Human TMPRSS2

Depositor

Sonja Best

Insert

TMPRSS2 (TMPRSS2 Human)

Use

Lentiviral

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pBABE-puro-NLS-LSSmKate2

Plasmid

#34586

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

NLS-LSS-mKate2

Use

Retroviral

Expression

Mammalian

Mutation

K69Y/P131T/S148G/M167D/T183S/M196V compared to mK…

Available Since

Feb. 21, 2012

Availability

Academic Institutions and Nonprofits only

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lenti-tmpknot-epegRNA-puro backbone

Plasmid

#214089

Purpose

Lentiviral backbone plasmid to insert epegRNAs (mpknot) of interest. The cloning site is BsmbI.

Depositor

Ting Zhou

Article

Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

hU6, EF1a

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-MPAct-mRuby3-IRES-Puro

Plasmid

#155231

Purpose

lentiviral MPAct construct

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

Lentiviral expression of MPAct-mRuby sensor

Use

Lentiviral

Tags

mRuby3

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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PLKO-mcherry-luc-puro-shSCR

Plasmid

#29780

Depositor

Carl Novina

Article

Levy et al Mol Cell. 2010 Dec 10. 40(5):841-9.

Insert

shSCR

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA4TO-K560-E236A-24xGCN4_v1-IRES-Puro

Plasmid

#60909

Purpose

A minimal K560 kinesin rigor motor tagged with the SunTag for single molecule imaging

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

K560-E236A

Expression

Mammalian

Available Since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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